Matches in SemOpenAlex for { <https://semopenalex.org/work/W301270488> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W301270488 endingPage "1750" @default.
- W301270488 startingPage "1744" @default.
- W301270488 abstract "Abstract Interactions between vascular endothelial cells and blood platelets have been investigated using a model microcirculation consisting of microcarrier beads colonized with human umbilical vein endothelial cells (HUVECs) and perfused with washed platelet suspensions. To simulate the effects of endothelial desquamation and exposure of subendothelium, fibrillar collagen in suspension was coinjected with the platelets. In this model, neither the passage of platelets alone nor collagen alone stimulated prostacyclin (PGI2) production by the HUVECs. Platelets activated by coinjection with collagen released thromboxane A2 (TXA2), and this was associated with the simultaneous production of PGI2 by the HUVECs. By means of double-isotope experiments with [3H]arachidonic acid (AA) incorporated into platelets and [14C]-AA into HUVECs, it was shown that all the PGI2 generated was derived from platelet AA and/or endoperoxides. This interpretation was strengthened by the finding that PGI2 production was not prevented by treatment of HUVECs with indomethacin followed by perfusion with collagen-stimulated platelets. AA metabolites in double-isotope label experiments were further characterized by reverse-phase chromatography, and it was shown that both cyclooxygenase and lipoxygenase products of the HUVECs were derived from platelet membrane lipid. Thrombin regularly produced transient PGI2 release, but showed rapid tachyphylaxis. Platelet-derived compounds including ADP, ATP, and platelet-activating factor (PAF) did not produce PGI2 release by HUVECs in this system. Thus, the transfer of AA and metabolites from collagen- stimulated platelets is likely to be the mechanism for PGI2 production in the context of minor degrees of endothelial desquamation." @default.
- W301270488 created "2016-06-24" @default.
- W301270488 creator A5003327249 @default.
- W301270488 creator A5017679097 @default.
- W301270488 creator A5023534456 @default.
- W301270488 creator A5029696484 @default.
- W301270488 date "1986-06-01" @default.
- W301270488 modified "2023-09-29" @default.
- W301270488 title "Substrate for endothelial prostacyclin production in the presence of platelets exposed to collagen is derived from the platelets rather than the endothelium" @default.
- W301270488 cites W1502563924 @default.
- W301270488 cites W1517088677 @default.
- W301270488 cites W1602579358 @default.
- W301270488 cites W1638102173 @default.
- W301270488 cites W1964537647 @default.
- W301270488 cites W1967932034 @default.
- W301270488 cites W1969559107 @default.
- W301270488 cites W1979833158 @default.
- W301270488 cites W1980581864 @default.
- W301270488 cites W1981159906 @default.
- W301270488 cites W1995716473 @default.
- W301270488 cites W1996578461 @default.
- W301270488 cites W2021367503 @default.
- W301270488 cites W2024373128 @default.
- W301270488 cites W2026079918 @default.
- W301270488 cites W2030768867 @default.
- W301270488 cites W2046365798 @default.
- W301270488 cites W2053806685 @default.
- W301270488 cites W2054583053 @default.
- W301270488 cites W2056818332 @default.
- W301270488 cites W2066615167 @default.
- W301270488 cites W2067635854 @default.
- W301270488 cites W2069847630 @default.
- W301270488 cites W2078745461 @default.
- W301270488 cites W2086826087 @default.
- W301270488 cites W2092971835 @default.
- W301270488 cites W2119518368 @default.
- W301270488 cites W2132184473 @default.
- W301270488 cites W2155256116 @default.
- W301270488 cites W2157603347 @default.
- W301270488 cites W2239854841 @default.
- W301270488 cites W2415914825 @default.
- W301270488 doi "https://doi.org/10.1182/blood.v67.6.1744.1744" @default.
- W301270488 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3085750" @default.
- W301270488 hasPublicationYear "1986" @default.
- W301270488 type Work @default.
- W301270488 sameAs 301270488 @default.
- W301270488 citedByCount "25" @default.
- W301270488 crossrefType "journal-article" @default.
- W301270488 hasAuthorship W301270488A5003327249 @default.
- W301270488 hasAuthorship W301270488A5017679097 @default.
- W301270488 hasAuthorship W301270488A5023534456 @default.
- W301270488 hasAuthorship W301270488A5029696484 @default.
- W301270488 hasBestOaLocation W3012704881 @default.
- W301270488 hasConcept C185592680 @default.
- W301270488 hasConcept C202751555 @default.
- W301270488 hasConcept C203014093 @default.
- W301270488 hasConcept C2776785769 @default.
- W301270488 hasConcept C2777292125 @default.
- W301270488 hasConcept C2777399203 @default.
- W301270488 hasConcept C2777411675 @default.
- W301270488 hasConcept C2781024287 @default.
- W301270488 hasConcept C2781128415 @default.
- W301270488 hasConcept C2993802102 @default.
- W301270488 hasConcept C55493867 @default.
- W301270488 hasConcept C86803240 @default.
- W301270488 hasConcept C89560881 @default.
- W301270488 hasConceptScore W301270488C185592680 @default.
- W301270488 hasConceptScore W301270488C202751555 @default.
- W301270488 hasConceptScore W301270488C203014093 @default.
- W301270488 hasConceptScore W301270488C2776785769 @default.
- W301270488 hasConceptScore W301270488C2777292125 @default.
- W301270488 hasConceptScore W301270488C2777399203 @default.
- W301270488 hasConceptScore W301270488C2777411675 @default.
- W301270488 hasConceptScore W301270488C2781024287 @default.
- W301270488 hasConceptScore W301270488C2781128415 @default.
- W301270488 hasConceptScore W301270488C2993802102 @default.
- W301270488 hasConceptScore W301270488C55493867 @default.
- W301270488 hasConceptScore W301270488C86803240 @default.
- W301270488 hasConceptScore W301270488C89560881 @default.
- W301270488 hasIssue "6" @default.
- W301270488 hasLocation W3012704881 @default.
- W301270488 hasOpenAccess W301270488 @default.
- W301270488 hasPrimaryLocation W3012704881 @default.
- W301270488 hasRelatedWork W1979953547 @default.
- W301270488 hasRelatedWork W2000748457 @default.
- W301270488 hasRelatedWork W2011944663 @default.
- W301270488 hasRelatedWork W2073909820 @default.
- W301270488 hasRelatedWork W2396218635 @default.
- W301270488 hasRelatedWork W2417996629 @default.
- W301270488 hasRelatedWork W2884989141 @default.
- W301270488 hasRelatedWork W293113631 @default.
- W301270488 hasRelatedWork W3139571160 @default.
- W301270488 hasRelatedWork W4240181524 @default.
- W301270488 hasVolume "67" @default.
- W301270488 isParatext "false" @default.
- W301270488 isRetracted "false" @default.
- W301270488 magId "301270488" @default.
- W301270488 workType "article" @default.