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- W3012732770 abstract "The selection and firing of DNA replication origins play key roles in ensuring that eukaryotes accurately replicate their genomes. This process is not well documented in plants due in large measure to difficulties in working with plant systems. We developed a new functional assay to label and map very early replicating loci that must, by definition, include at least a subset of replication origins. Arabidopsis (Arabidopsis thaliana) cells were briefly labeled with 5-ethynyl-2′-deoxy-uridine, and nuclei were subjected to two-parameter flow sorting. We identified more than 5500 loci as initiation regions (IRs), the first regions to replicate in very early S phase. These were classified as strong or weak IRs based on the strength of their replication signals. Strong initiation regions were evenly spaced along chromosomal arms and depleted in centromeres, while weak initiation regions were enriched in centromeric regions. IRs are AT-rich sequences flanked by more GC-rich regions and located predominantly in intergenic regions. Nuclease sensitivity assays indicated that IRs are associated with accessible chromatin. Based on these observations, initiation of plant DNA replication shows some similarity to, but is also distinct from, initiation in other well-studied eukaryotic systems." @default.
- W3012732770 created "2020-03-27" @default.
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- W3012732770 date "2020-03-23" @default.
- W3012732770 modified "2023-10-12" @default.
- W3012732770 title "Arabidopsis DNA Replication Initiates in Intergenic, AT-Rich Open Chromatin" @default.
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- W3012732770 doi "https://doi.org/10.1104/pp.19.01520" @default.
- W3012732770 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7210620" @default.
- W3012732770 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32205451" @default.
- W3012732770 hasPublicationYear "2020" @default.
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