Matches in SemOpenAlex for { <https://semopenalex.org/work/W3012930547> ?p ?o ?g. }
- W3012930547 endingPage "777" @default.
- W3012930547 startingPage "777" @default.
- W3012930547 abstract "Ubiquitination regulates several biological processes, however the role of specific members of the ubiquitinome on intracellular membrane trafficking is not yet fully understood. Here, we search for ubiquitin-related genes implicated in protein membrane trafficking performing a High-Content siRNA Screening including 1187 genes of the human “ubiquitinome” using amyloid precursor protein (APP) as a reporter. We identified the deubiquitinating enzyme PSMD14, a subunit of the 19S regulatory particle of the proteasome, specific for K63-Ub chains in cells, as a novel regulator of Golgi-to-endoplasmic reticulum (ER) retrograde transport. Silencing or pharmacological inhibition of PSMD14 with Capzimin (CZM) caused a robust increase in APP levels at the Golgi apparatus and the swelling of this organelle. We showed that this phenotype is the result of rapid inhibition of Golgi-to-ER retrograde transport, a pathway implicated in the early steps of the autophagosomal formation. Indeed, we observed that inhibition of PSMD14 with CZM acts as a potent blocker of macroautophagy by a mechanism related to the retention of Atg9A and Rab1A at the Golgi apparatus. As pharmacological inhibition of the proteolytic core of the 20S proteasome did not recapitulate these effects, we concluded that PSMD14, and the K63-Ub chains, act as a crucial regulatory factor for macroautophagy by controlling Golgi-to-ER retrograde transport." @default.
- W3012930547 created "2020-03-27" @default.
- W3012930547 creator A5002095641 @default.
- W3012930547 creator A5009574816 @default.
- W3012930547 creator A5018525114 @default.
- W3012930547 creator A5019428194 @default.
- W3012930547 creator A5029452323 @default.
- W3012930547 creator A5030661025 @default.
- W3012930547 creator A5043387701 @default.
- W3012930547 creator A5048483407 @default.
- W3012930547 creator A5050621605 @default.
- W3012930547 creator A5051617978 @default.
- W3012930547 creator A5051652752 @default.
- W3012930547 creator A5059634563 @default.
- W3012930547 creator A5068968280 @default.
- W3012930547 creator A5070050916 @default.
- W3012930547 creator A5074408402 @default.
- W3012930547 creator A5082818837 @default.
- W3012930547 creator A5088451627 @default.
- W3012930547 date "2020-03-23" @default.
- W3012930547 modified "2023-10-18" @default.
- W3012930547 title "The Proteasomal Deubiquitinating Enzyme PSMD14 Regulates Macroautophagy by Controlling Golgi-to-ER Retrograde Transport" @default.
- W3012930547 cites W1591297143 @default.
- W3012930547 cites W1964677366 @default.
- W3012930547 cites W1969963064 @default.
- W3012930547 cites W1971444034 @default.
- W3012930547 cites W1977502249 @default.
- W3012930547 cites W1977767269 @default.
- W3012930547 cites W1985274903 @default.
- W3012930547 cites W1986303112 @default.
- W3012930547 cites W1989422232 @default.
- W3012930547 cites W1991296165 @default.
- W3012930547 cites W1992515094 @default.
- W3012930547 cites W1993712760 @default.
- W3012930547 cites W1998079639 @default.
- W3012930547 cites W2005722151 @default.
- W3012930547 cites W2007277647 @default.
- W3012930547 cites W2015709967 @default.
- W3012930547 cites W2016866607 @default.
- W3012930547 cites W2031978571 @default.
- W3012930547 cites W2032186831 @default.
- W3012930547 cites W2035171221 @default.
- W3012930547 cites W2035379303 @default.
- W3012930547 cites W2040732053 @default.
- W3012930547 cites W2042027043 @default.
- W3012930547 cites W2042158953 @default.
- W3012930547 cites W2043413606 @default.
- W3012930547 cites W2044347239 @default.
- W3012930547 cites W2046474853 @default.
- W3012930547 cites W2050745715 @default.
- W3012930547 cites W2060354742 @default.
- W3012930547 cites W2061296359 @default.
- W3012930547 cites W2063736701 @default.
- W3012930547 cites W2066972887 @default.
- W3012930547 cites W2068213179 @default.
- W3012930547 cites W2068696470 @default.
- W3012930547 cites W2070476685 @default.
- W3012930547 cites W2071374729 @default.
- W3012930547 cites W2074911710 @default.
- W3012930547 cites W2082143856 @default.
- W3012930547 cites W2090926709 @default.
- W3012930547 cites W2098912234 @default.
- W3012930547 cites W2099634214 @default.
- W3012930547 cites W2103059759 @default.
- W3012930547 cites W2103552916 @default.
- W3012930547 cites W2107277218 @default.
- W3012930547 cites W2108244474 @default.
- W3012930547 cites W2113736802 @default.
- W3012930547 cites W2115246685 @default.
- W3012930547 cites W2118225942 @default.
- W3012930547 cites W2124583600 @default.
- W3012930547 cites W2125798501 @default.
- W3012930547 cites W2127071017 @default.
- W3012930547 cites W2127120815 @default.
- W3012930547 cites W2127984419 @default.
- W3012930547 cites W2137575965 @default.
- W3012930547 cites W2141516006 @default.
- W3012930547 cites W2142047537 @default.
- W3012930547 cites W2147917253 @default.
- W3012930547 cites W2148566355 @default.
- W3012930547 cites W2150159833 @default.
- W3012930547 cites W2158574194 @default.
- W3012930547 cites W2162223351 @default.
- W3012930547 cites W2165805293 @default.
- W3012930547 cites W2169825983 @default.
- W3012930547 cites W2169950973 @default.
- W3012930547 cites W2193855830 @default.
- W3012930547 cites W2271324282 @default.
- W3012930547 cites W2343251627 @default.
- W3012930547 cites W2467513012 @default.
- W3012930547 cites W2514327877 @default.
- W3012930547 cites W2529526688 @default.
- W3012930547 cites W2560345632 @default.
- W3012930547 cites W2594482234 @default.
- W3012930547 cites W2599967769 @default.
- W3012930547 cites W2602775024 @default.
- W3012930547 cites W2606621683 @default.
- W3012930547 cites W2613065881 @default.