FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3013119678> ?p ?o ?g. }

• W3013119678 abstract "ABSTRACT The oxylipins, 7S,14S-diHDHA and 7S,17S-diHDHA (RvD5), have been found in macrophages exudates and are believed to function as specialized pro-resolving mediators (SPM’s). Their biosynthesis is thought to proceed through sequential oxidations of docosahexaenoic acid (DHA) by lipoxygenase enzymes, specifically by h5-LOX first to 7S-HDHA, followed by h12-LOX to form 7S,14S-diHDHA or h15-LOX-1 to form 7S,17S-diHDHA (RvD5). In this work, we determined that oxidation of 7S-HpDHA to 7S,14S-diHDHA can be performed by either h12-LOX or h15-LOX-1, with similar kinetics. The oxidation at C14 of DHA by h12-LOX was expected, but the non-canonical reaction of h15-LOX-1 to make primarily 7S,14S-diHDHA was unexpected. Computer modeling suggests the alcohol on C7 of 7S-HDHA hydrogen bonds with the backbone carbonyl of I399, forcing the hydrogen abstraction from C12 to oxygenate on C14, and not C17. This result raised questions regarding synthesis of 7S,17S-diHDHA (RvD5). Strikingly, we find h15-LOX-2 oxygenates 7S-HDHA almost exclusively at C17, forming RvD5 with faster kinetics than h15-LOX-1. The presence of h15-LOX-2 in neutrophils and macrophages, suggests it may have a greater role in biosynthesizing SPM’s than previously thought. We also determined that the reactions of h5-LOX with 14S-HpDHA and 17S-HpDHA are kinetically slow compared to DHA, suggesting these may be minor biosynthetic routes in-vivo . Additionally, we show that 7S,14S-diHDHA and RvD5 have anti-aggregation properties with platelets at low micro-molar potencies, which could directly regulate clot resolution." @default.
• W3013119678 created "2020-04-03" @default.
• W3013119678 creator A5001224002 @default.
• W3013119678 creator A5051041892 @default.
• W3013119678 creator A5055041808 @default.
• W3013119678 creator A5068160025 @default.
• W3013119678 creator A5070292545 @default.
• W3013119678 creator A5086679528 @default.
• W3013119678 creator A5088307385 @default.
• W3013119678 creator A5088764798 @default.
• W3013119678 creator A5089351110 @default.
• W3013119678 date "2020-03-25" @default.
• W3013119678 modified "2023-09-23" @default.
• W3013119678 title "Altered specificity of 15-LOX-1 in the biosynthesis of 7S,14S-diHDHA implicates 15-LOX-2 in biosynthesis of resolvin D5" @default.
• W3013119678 cites W112876996 @default.
• W3013119678 cites W1488735754 @default.
• W3013119678 cites W1531198291 @default.
• W3013119678 cites W1604010542 @default.
• W3013119678 cites W1668867309 @default.
• W3013119678 cites W1878686232 @default.
• W3013119678 cites W1959056525 @default.
• W3013119678 cites W1967107808 @default.
• W3013119678 cites W1978704058 @default.
• W3013119678 cites W1985588649 @default.
• W3013119678 cites W1989123782 @default.
• W3013119678 cites W1989419220 @default.
• W3013119678 cites W1997815363 @default.
• W3013119678 cites W1999682234 @default.
• W3013119678 cites W2003296127 @default.
• W3013119678 cites W2007082033 @default.
• W3013119678 cites W2008331938 @default.
• W3013119678 cites W2012165749 @default.
• W3013119678 cites W2017234831 @default.
• W3013119678 cites W2018321409 @default.
• W3013119678 cites W2020121125 @default.
• W3013119678 cites W2020466729 @default.
• W3013119678 cites W2021836275 @default.
• W3013119678 cites W2022439153 @default.
• W3013119678 cites W2028705982 @default.
• W3013119678 cites W2029004344 @default.
• W3013119678 cites W2030126029 @default.
• W3013119678 cites W2030431061 @default.
• W3013119678 cites W2031000812 @default.
• W3013119678 cites W2031155614 @default.
• W3013119678 cites W2045307713 @default.
• W3013119678 cites W2045657732 @default.
• W3013119678 cites W2048310961 @default.
• W3013119678 cites W2048855990 @default.
• W3013119678 cites W2048922130 @default.
• W3013119678 cites W2059445538 @default.
• W3013119678 cites W2064766999 @default.
• W3013119678 cites W2073202677 @default.
• W3013119678 cites W2074203890 @default.
• W3013119678 cites W2074780993 @default.
• W3013119678 cites W2076898547 @default.
• W3013119678 cites W2082649626 @default.
• W3013119678 cites W2082973874 @default.
• W3013119678 cites W2086790252 @default.
• W3013119678 cites W2089162053 @default.
• W3013119678 cites W2091761903 @default.
• W3013119678 cites W2097685656 @default.
• W3013119678 cites W2103010344 @default.
• W3013119678 cites W2107821069 @default.
• W3013119678 cites W2117981187 @default.
• W3013119678 cites W2121737195 @default.
• W3013119678 cites W2121949424 @default.
• W3013119678 cites W2122405246 @default.
• W3013119678 cites W2140616220 @default.
• W3013119678 cites W2148536296 @default.
• W3013119678 cites W2152443396 @default.
• W3013119678 cites W2155474314 @default.
• W3013119678 cites W2156416313 @default.
• W3013119678 cites W2157374284 @default.
• W3013119678 cites W2165473528 @default.
• W3013119678 cites W2275244318 @default.
• W3013119678 cites W2287654917 @default.
• W3013119678 cites W2315873193 @default.
• W3013119678 cites W2316344275 @default.
• W3013119678 cites W2345962422 @default.
• W3013119678 cites W2473287368 @default.
• W3013119678 cites W2491676614 @default.
• W3013119678 cites W2580987090 @default.
• W3013119678 cites W2605253263 @default.
• W3013119678 cites W2616486090 @default.
• W3013119678 cites W2673025512 @default.
• W3013119678 cites W2739568820 @default.
• W3013119678 cites W2791298178 @default.
• W3013119678 cites W2791739231 @default.
• W3013119678 cites W2885613894 @default.
• W3013119678 cites W2886317149 @default.
• W3013119678 cites W2888718738 @default.
• W3013119678 cites W2899683939 @default.
• W3013119678 cites W4237669910 @default.
• W3013119678 cites W4241732879 @default.
• W3013119678 doi "https://doi.org/10.1101/2020.03.25.008573" @default.
• W3013119678 hasPublicationYear "2020" @default.
• W3013119678 type Work @default.
• W3013119678 sameAs 3013119678 @default.
• W3013119678 citedByCount "0" @default.
• W3013119678 crossrefType "posted-content" @default.

• next