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- W3013124927 endingPage "5881" @default.
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- W3013124927 abstract "Comprehensive determination of primary sequence and identification of post-translational modifications (PTMs) are key elements in protein structural analysis. Various mass spectrometry (MS) based fragmentation techniques are powerful approaches for mapping both the amino acid sequence and PTMs; one of these techniques is matrix-assisted laser desorption/ionization (MALDI), combined with in-source decay (ISD) fragmentation and Fourier-transform ion cyclotron resonance (FT-ICR) MS. MALDI-ISD MS protein analysis involves only minimal sample preparation and does not require spectral deconvolution. The resulting MALDI-ISD MS data is complementary to electrospray ionization-based MS/MS sequencing readouts, providing knowledge on the types of fragment ions is available. In this study, we evaluate the isotopic distributions of z′ ions in protein top-down MALDI-ISD FT-ICR mass spectra and show why these distributions can deviate from theoretical profiles as a result of co-occurring and isomeric z and y-NH3 ions. Two synthetic peptides, containing either normal or deuterated alanine residues, were used to confirm the presence and unravel the identity of isomeric z and y-NH3 fragment ions (“twins”). Furthermore, two reducing MALDI matrices, namely 1,5-diaminonaphthalene and N-phenyl-p-phenylenediamine were applied that yield ISD mass spectra with different fragment ion distributions. This study demonstrates that the relative abundance of isomeric z and y-NH3 ions requires consideration for accurate and confident assignments of z′ ions in MALDI-ISD FT-ICR mass spectra." @default.
- W3013124927 created "2020-04-03" @default.
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- W3013124927 creator A5072482063 @default.
- W3013124927 date "2020-03-26" @default.
- W3013124927 modified "2023-09-26" @default.
- W3013124927 title "Evaluation of Sibling and Twin Fragment Ions Improves the Structural Characterization of Proteins by Top-Down MALDI In-Source Decay Mass Spectrometry" @default.
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- W3013124927 doi "https://doi.org/10.1021/acs.analchem.9b05683" @default.
- W3013124927 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7178258" @default.
- W3013124927 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32212639" @default.
- W3013124927 hasPublicationYear "2020" @default.
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