Matches in SemOpenAlex for { <https://semopenalex.org/work/W3013178722> ?p ?o ?g. }
- W3013178722 abstract "Asparaginyl endopeptidases (AEPs) catalyze the key backbone cyclization step during the biosynthesis of plant-derived cyclic peptides. Here, we report the identification of two AEPs from Momordica cochinchinensis and biochemically characterize MCoAEP2 that catalyzes the maturation of trypsin inhibitor cyclotides. Recombinantly produced MCoAEP2 catalyzes the backbone cyclization of a linear cyclotide precursor (MCoTI-II-NAL) with a kcat/Km of 620 mM-1 s-1, making it one of the fastest cyclases reported to date. We show that MCoAEP2 can mediate both the N-terminal excision and C-terminal cyclization of cyclotide precursors in vitro. The rate of cyclization/hydrolysis is primarily influenced by varying pH, which could potentially control the succession of AEP-mediated processing events in vivo. Furthermore, MCoAEP2 efficiently catalyzes the backbone cyclization of an engineered MCoTI-II analog with anti-angiogenic activity. MCoAEP2 provides enhanced synthetic access to structures previously inaccessible by direct chemistry approaches and enables the wider application of trypsin inhibitor cyclotides in biotechnology applications." @default.
- W3013178722 created "2020-04-03" @default.
- W3013178722 creator A5003562114 @default.
- W3013178722 creator A5005935031 @default.
- W3013178722 creator A5008575244 @default.
- W3013178722 creator A5014444837 @default.
- W3013178722 creator A5014704784 @default.
- W3013178722 creator A5021506134 @default.
- W3013178722 creator A5028427729 @default.
- W3013178722 creator A5073722103 @default.
- W3013178722 creator A5079308758 @default.
- W3013178722 creator A5087029469 @default.
- W3013178722 date "2020-03-27" @default.
- W3013178722 modified "2023-10-15" @default.
- W3013178722 title "A bifunctional asparaginyl endopeptidase efficiently catalyzes both cleavage and cyclization of cyclic trypsin inhibitors" @default.
- W3013178722 cites W1555403344 @default.
- W3013178722 cites W1557806686 @default.
- W3013178722 cites W1699671485 @default.
- W3013178722 cites W1817886125 @default.
- W3013178722 cites W1964349266 @default.
- W3013178722 cites W1964939637 @default.
- W3013178722 cites W1974384503 @default.
- W3013178722 cites W1995390287 @default.
- W3013178722 cites W1999613945 @default.
- W3013178722 cites W2011301103 @default.
- W3013178722 cites W2025405581 @default.
- W3013178722 cites W2029582401 @default.
- W3013178722 cites W2048797519 @default.
- W3013178722 cites W2049989028 @default.
- W3013178722 cites W2051343450 @default.
- W3013178722 cites W2054798478 @default.
- W3013178722 cites W2054945796 @default.
- W3013178722 cites W2055043387 @default.
- W3013178722 cites W2064453331 @default.
- W3013178722 cites W2065283382 @default.
- W3013178722 cites W2085622333 @default.
- W3013178722 cites W2093071815 @default.
- W3013178722 cites W2094142441 @default.
- W3013178722 cites W2105624689 @default.
- W3013178722 cites W2106190285 @default.
- W3013178722 cites W2108175440 @default.
- W3013178722 cites W2126419817 @default.
- W3013178722 cites W2131271579 @default.
- W3013178722 cites W2133301540 @default.
- W3013178722 cites W2142623863 @default.
- W3013178722 cites W2145983591 @default.
- W3013178722 cites W2147993766 @default.
- W3013178722 cites W2162854463 @default.
- W3013178722 cites W2169707043 @default.
- W3013178722 cites W2171257497 @default.
- W3013178722 cites W2191442969 @default.
- W3013178722 cites W2267190811 @default.
- W3013178722 cites W2330799739 @default.
- W3013178722 cites W2397734123 @default.
- W3013178722 cites W2404280981 @default.
- W3013178722 cites W2488071591 @default.
- W3013178722 cites W2511955955 @default.
- W3013178722 cites W2522742593 @default.
- W3013178722 cites W2530816040 @default.
- W3013178722 cites W2587985366 @default.
- W3013178722 cites W2592527354 @default.
- W3013178722 cites W2594877435 @default.
- W3013178722 cites W2599154314 @default.
- W3013178722 cites W2614400304 @default.
- W3013178722 cites W2761775375 @default.
- W3013178722 cites W2779298143 @default.
- W3013178722 cites W2786057985 @default.
- W3013178722 cites W2787400035 @default.
- W3013178722 cites W2788197842 @default.
- W3013178722 cites W2795470773 @default.
- W3013178722 cites W2802911639 @default.
- W3013178722 cites W2808752558 @default.
- W3013178722 cites W2891585482 @default.
- W3013178722 cites W2900787783 @default.
- W3013178722 cites W2931364513 @default.
- W3013178722 cites W2945590934 @default.
- W3013178722 cites W2946803285 @default.
- W3013178722 cites W2963311769 @default.
- W3013178722 cites W3217567697 @default.
- W3013178722 doi "https://doi.org/10.1038/s41467-020-15418-2" @default.
- W3013178722 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7101308" @default.
- W3013178722 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32221295" @default.
- W3013178722 hasPublicationYear "2020" @default.
- W3013178722 type Work @default.
- W3013178722 sameAs 3013178722 @default.
- W3013178722 citedByCount "57" @default.
- W3013178722 countsByYear W30131787222020 @default.
- W3013178722 countsByYear W30131787222021 @default.
- W3013178722 countsByYear W30131787222022 @default.
- W3013178722 countsByYear W30131787222023 @default.
- W3013178722 crossrefType "journal-article" @default.
- W3013178722 hasAuthorship W3013178722A5003562114 @default.
- W3013178722 hasAuthorship W3013178722A5005935031 @default.
- W3013178722 hasAuthorship W3013178722A5008575244 @default.
- W3013178722 hasAuthorship W3013178722A5014444837 @default.
- W3013178722 hasAuthorship W3013178722A5014704784 @default.
- W3013178722 hasAuthorship W3013178722A5021506134 @default.
- W3013178722 hasAuthorship W3013178722A5028427729 @default.
- W3013178722 hasAuthorship W3013178722A5073722103 @default.
- W3013178722 hasAuthorship W3013178722A5079308758 @default.