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- W3013255710 abstract "Abstract Beta cell failure and apoptosis following islet inflammation have been associated with autoimmune type 1 diabetes pathogenesis. As conveyors of biological active material, extracellular vesicles (EV) act as mediators in communication with immune effectors fostering the idea that EV from inflamed beta cells may contribute to autoimmunity. Evidence accumulates that beta exosomes promote diabetogenic responses, but relative contributions of larger vesicles as well as variations in the composition of the beta cell’s vesiculome due to environmental changes have not been explored yet. Here, we made side-by-side comparisons of the phenotype and function of apoptotic bodies (AB), microvesicles (MV) and small EV (sEV) isolated from an equal amount of MIN6 beta cells exposed to inflammatory, hypoxic or genotoxic stressors. Under normal conditions, large vesicles represent 93% of the volume, but only 2% of the number of the vesicles. Our data reveal a consistently higher release of AB and sEV and to a lesser extent of MV, exclusively under inflammatory conditions commensurate with a 4-fold increase in the total volume of the vesiculome and enhanced export of immune-stimulatory material including the autoantigen insulin, microRNA, and cytokines. Whilst inflammation does not change the concentration of insulin inside the EV, specific Toll-like receptor-binding microRNA sequences preferentially partition into sEV. Exposure to inflammatory stress engenders drastic increases in the expression of monocyte chemoattractant protein 1 in all EV and of interleukin-27 solely in AB suggesting selective sorting towards EV subspecies. Functional in vitro assays in mouse dendritic cells and macrophages reveal further differences in the aptitude of EV to modulate expression of cytokines and maturation markers. These findings highlight the different quantitative and qualitative imprints of environmental changes in subpopulations of beta EV that may contribute to the spread of inflammation and sustained immune cell recruitment at the inception of the (auto-) immune response. Graphical Abstract Inflammation stimulates release of a heterogeneous population of beta EV with differential expression of immunogenic substances involved in immune cell recruitment and activation. Highlights Stress engenders an up to four-fold increase in the volume of the vesiculome and enhanced auto-antigen release Cytokines are selectively sorted into EV subspecies TLR-binding microRNAs are enriched in sEV EV from stressed beta cells promote dendritic and macrophage cell activation" @default.
- W3013255710 created "2020-04-03" @default.
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- W3013255710 date "2020-03-29" @default.
- W3013255710 modified "2023-09-30" @default.
- W3013255710 title "Molecular and functional diversity of distinct subpopulations of extracellular vesicles from stressed pancreatic beta cells: implications for autoimmunity" @default.
- W3013255710 cites W144423133 @default.
- W3013255710 cites W1524269662 @default.
- W3013255710 cites W1742956183 @default.
- W3013255710 cites W1863508629 @default.
- W3013255710 cites W1877686344 @default.
- W3013255710 cites W1965748339 @default.
- W3013255710 cites W1967343005 @default.
- W3013255710 cites W1975227291 @default.
- W3013255710 cites W1977994841 @default.
- W3013255710 cites W1978278335 @default.
- W3013255710 cites W2007499410 @default.
- W3013255710 cites W2012962101 @default.
- W3013255710 cites W2020863352 @default.
- W3013255710 cites W2024108088 @default.
- W3013255710 cites W2029101220 @default.
- W3013255710 cites W2030994794 @default.
- W3013255710 cites W2039420809 @default.
- W3013255710 cites W2040635479 @default.
- W3013255710 cites W2051532609 @default.
- W3013255710 cites W2052853635 @default.
- W3013255710 cites W2055313665 @default.
- W3013255710 cites W2057419005 @default.
- W3013255710 cites W2058404113 @default.
- W3013255710 cites W2062573090 @default.
- W3013255710 cites W2085609942 @default.
- W3013255710 cites W2087032228 @default.
- W3013255710 cites W2092787543 @default.
- W3013255710 cites W2096921043 @default.
- W3013255710 cites W2098760341 @default.
- W3013255710 cites W2099261166 @default.
- W3013255710 cites W2103302057 @default.
- W3013255710 cites W2111298415 @default.
- W3013255710 cites W2111979626 @default.
- W3013255710 cites W2133644385 @default.
- W3013255710 cites W2133771702 @default.
- W3013255710 cites W2139685116 @default.
- W3013255710 cites W2141063928 @default.
- W3013255710 cites W2145869245 @default.
- W3013255710 cites W2154876324 @default.
- W3013255710 cites W2156167884 @default.
- W3013255710 cites W2157786523 @default.
- W3013255710 cites W2165473528 @default.
- W3013255710 cites W2165607564 @default.
- W3013255710 cites W2166469044 @default.
- W3013255710 cites W2169894204 @default.
- W3013255710 cites W2194131658 @default.
- W3013255710 cites W2338777971 @default.
- W3013255710 cites W2416473596 @default.
- W3013255710 cites W2416738232 @default.
- W3013255710 cites W2509057555 @default.
- W3013255710 cites W2554427090 @default.
- W3013255710 cites W2555906390 @default.
- W3013255710 cites W2557771720 @default.
- W3013255710 cites W2561975906 @default.
- W3013255710 cites W2582409952 @default.
- W3013255710 cites W2586979555 @default.
- W3013255710 cites W2593684819 @default.
- W3013255710 cites W2729928936 @default.
- W3013255710 cites W2782479267 @default.
- W3013255710 cites W2786514708 @default.
- W3013255710 cites W2789819037 @default.
- W3013255710 cites W2792549042 @default.
- W3013255710 cites W2807051424 @default.
- W3013255710 cites W2810413556 @default.
- W3013255710 cites W2891767442 @default.
- W3013255710 cites W2895431429 @default.
- W3013255710 cites W2896348655 @default.
- W3013255710 cites W2899337283 @default.
- W3013255710 cites W2900756670 @default.
- W3013255710 cites W2911854480 @default.
- W3013255710 cites W2950757810 @default.
- W3013255710 cites W2950891082 @default.
- W3013255710 cites W2965274533 @default.
- W3013255710 cites W2966119663 @default.
- W3013255710 cites W2970715880 @default.
- W3013255710 cites W2993246221 @default.
- W3013255710 cites W2995149669 @default.
- W3013255710 doi "https://doi.org/10.1101/2020.03.26.003145" @default.
- W3013255710 hasPublicationYear "2020" @default.
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