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• W3013641881 endingPage "1097" @default.
• W3013641881 startingPage "1085" @default.
• W3013641881 abstract "Clinicians prescribe hundreds of millions of β-lactam antibiotics to treat the majority of patients presenting with bacterial infections. Patient outcomes are positive unless resistant bacteria, such as Pseudomonas aeruginosa (P. aeruginosa), are present. P. aeruginosa has both intrinsic and acquired antibiotic resistance, making clinical management of infection a real challenge, particularly when these bacteria are sequestered in biofilms. These problems would be alleviated if, upon the initial presentation of bacterial infection symptoms, clinicians were able to administer an antibiotic that kills both susceptible and otherwise resistant bacteria and eradicates biofilms. As the most common class of antibiotics, β-lactams could be used in a new drug if the leading causes of β-lactam antibiotic resistance, permeation barriers from lipopolysaccharide, efflux pumps, and β-lactamase enzymes, were also defeated. Against P. aeruginosa and their biofilms, the potency of β-lactam antibiotics is restored with 600 Da branched polyethylenimine (600 Da BPEI). Checkerboard assays using microtiter plates demonstrate the potentiation of piperacillin, cefepime, Meropenem, and erythromycin antibiotics. Growth curves demonstrate that only a combination of 600 Da BPEI and piperacillin produces growth inhibition against antibiotic resistant P. aeruginosa. Scanning electron microscopy (SEM) was used to confirm that the combination treatment leads to abnormal P. aeruginosa morphology. Data collected with isothermal titration calorimetry and fluorescence spectroscopy demonstrate a mechanism of action in which potentiation at low concentrations of 600 Da BPEI reduces diffusion barriers from lipopolysaccharides without disrupting the outer membrane itself. Coupled with the ability to overcome a reduction in antibiotic activity created by biofilm exopolymers, targeting anionic sites on lipopolysaccharides and biofilm exopolysaccharides with the same compound provides new opportunities to counter the rise of multidrug-resistant infections." @default.
• W3013641881 created "2020-04-03" @default.
• W3013641881 creator A5004357012 @default.
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• W3013641881 date "2020-03-30" @default.
• W3013641881 modified "2023-10-17" @default.
• W3013641881 title "Overcoming Multidrug Resistance and Biofilms of <i>Pseudomonas aeruginosa</i> with a Single Dual-Function Potentiator of β-Lactams" @default.
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• W3013641881 cites W1680383394 @default.
• W3013641881 cites W1796861945 @default.
• W3013641881 cites W1905008412 @default.
• W3013641881 cites W1965006954 @default.
• W3013641881 cites W1966478139 @default.
• W3013641881 cites W1973197368 @default.
• W3013641881 cites W1981366496 @default.
• W3013641881 cites W1990155455 @default.
• W3013641881 cites W2002209858 @default.
• W3013641881 cites W2002616011 @default.
• W3013641881 cites W2003343094 @default.
• W3013641881 cites W2004602806 @default.
• W3013641881 cites W2007084462 @default.
• W3013641881 cites W2011602346 @default.
• W3013641881 cites W2013764931 @default.
• W3013641881 cites W2017848270 @default.
• W3013641881 cites W2039258127 @default.
• W3013641881 cites W2039729207 @default.
• W3013641881 cites W2043955978 @default.
• W3013641881 cites W2046700614 @default.
• W3013641881 cites W2052525877 @default.
• W3013641881 cites W2053628926 @default.
• W3013641881 cites W2056064788 @default.
• W3013641881 cites W2060361581 @default.
• W3013641881 cites W2069087157 @default.
• W3013641881 cites W2080936059 @default.
• W3013641881 cites W2087920996 @default.
• W3013641881 cites W2088808467 @default.
• W3013641881 cites W2098326603 @default.
• W3013641881 cites W2098785211 @default.
• W3013641881 cites W2101812185 @default.
• W3013641881 cites W2106351298 @default.
• W3013641881 cites W2111698236 @default.
• W3013641881 cites W2116814997 @default.
• W3013641881 cites W2120738727 @default.
• W3013641881 cites W2124439712 @default.
• W3013641881 cites W2127206088 @default.
• W3013641881 cites W2136766031 @default.
• W3013641881 cites W2138931089 @default.
• W3013641881 cites W2139441606 @default.
• W3013641881 cites W2141436568 @default.
• W3013641881 cites W2142829150 @default.
• W3013641881 cites W2142919688 @default.
• W3013641881 cites W2143147188 @default.
• W3013641881 cites W2144440398 @default.
• W3013641881 cites W2147358435 @default.
• W3013641881 cites W2149353640 @default.
• W3013641881 cites W2152408217 @default.
• W3013641881 cites W2155244175 @default.
• W3013641881 cites W2158463163 @default.
• W3013641881 cites W2159779488 @default.
• W3013641881 cites W2212629349 @default.
• W3013641881 cites W2293816157 @default.
• W3013641881 cites W2324039175 @default.
• W3013641881 cites W2402202411 @default.
• W3013641881 cites W2407589753 @default.
• W3013641881 cites W2414669751 @default.
• W3013641881 cites W2475270489 @default.
• W3013641881 cites W2518579334 @default.
• W3013641881 cites W2592451861 @default.
• W3013641881 cites W2594019989 @default.
• W3013641881 cites W2604420093 @default.
• W3013641881 cites W2632440743 @default.
• W3013641881 cites W2742847077 @default.
• W3013641881 cites W2754578981 @default.
• W3013641881 cites W2755234983 @default.
• W3013641881 cites W2755377931 @default.
• W3013641881 cites W2756826923 @default.
• W3013641881 cites W2763715289 @default.
• W3013641881 cites W2766155960 @default.
• W3013641881 cites W2793220261 @default.
• W3013641881 cites W2806191867 @default.
• W3013641881 cites W2890383291 @default.
• W3013641881 cites W2890592881 @default.
• W3013641881 cites W2969690891 @default.
• W3013641881 cites W2969837724 @default.
• W3013641881 cites W4241246544 @default.
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• W3013641881 doi "https://doi.org/10.1021/acsinfecdis.9b00486" @default.
• W3013641881 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7233300" @default.
• W3013641881 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32223216" @default.
• W3013641881 hasPublicationYear "2020" @default.
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