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• W3013661389 abstract "Cattle fatty liver has caused mass damage in milk production during the past few years. In our study, to identify different miRNAs involved in cell physiological regulation in fatty liver, we performed miRNA deep sequencing on a normal liver cell (S01), fatty liver cell (S02) and processed cell by monoammonium glycyrrhizinate (S03). As a result, a total of 15,277,462, 14,190,360 and 13,771,060 raw reads representing 13,904,074, 12,784,128 and 11,017,604 clean reads per library were obtained separately. Through bioinformatics analysis, a total of 511 known miRNAs were identified when they were aligned with the known animal miRNAs, and 197 novel miRNAs were predicted using mirDeep2 software. A total of 511 miRNAs including 101 known and 51 novel miRNAs were expressed significantly different. Additionally, expression levels of eight randomly selected miRNAs were confirmed using the stem-loop qPCR, and their expression profiles were consistent with the deep sequencing results. For better understanding the functions of miRNAs, a total of 14,231 targets were predicted. These predicted target genes were further analyzed by function annotation and enrichment pathways, the results showed that these targets of the identified miRNAs are involved in a broad range of physiological functions." @default.
• W3013661389 created "2020-04-03" @default.
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• W3013661389 date "2020-03-26" @default.
• W3013661389 modified "2023-10-18" @default.
• W3013661389 title "Identification of differentially expressed miRNAs on normal cell, fatty liver cell and processed cell by monoammonium glycyrrhizinate from cattle (Bos indicus) by deep sequencing approach" @default.
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• W3013661389 doi "https://doi.org/10.1080/10495398.2020.1744617" @default.
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