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- W3013695655 abstract "The ability to detect cancer biomarkers at the single-moleculelevel promises the ultimate sensitivity in clinical diagnosis.Fluorescence-based single-molecule analysis, however, islimited by matrix interferences, and therefore is typically notsuitable for real world analytical applications. This opticalbackground can be elegantly avoided by usingphoton-upconversion nanoparticles (UCNPs) that emitshorter-wavelength light under near-infrared (NIR) excitation(anti-Stokes emission). Conjugates of UCNPs with antibody canbe used as a background-free luminescent tracer inupconversion-linked immunosensing. We have developed an opticalapproach for visualizing individual UCNPs by conventionalepiluminescence microscopy and applied it for the sensitivedetection of the cancer biomarker prostate specific antigen(PSA). Individual sandwich immunocomplexes consisting of (1)immobilized anti-PSA antibody, (2) PSA, and (3) anti-PSAantibody-UCNP conjugate were counted under an upconversionwide-field microscope equipped with a 980 nm laser excitationsource. The noise-surpassing digital readout provided singleparticle resolution with almost no instrumental background.This allowed to reach a limit of detection (LOD) of 1.2 pg mL−1(42 fM) of PSA in 25 % blood serum which is about ten timesmore sensitive than a classical (analog) readout ofluminescence intensity." @default.
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- W3013695655 date "2018-01-01" @default.
- W3013695655 modified "2023-09-24" @default.
- W3013695655 title "Single-molecule upconversion-linked digital immunosensing ofcancer biomarkers" @default.
- W3013695655 hasPublicationYear "2018" @default.
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