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- W3013782880 abstract "Squalene synthase (SQS) catalyzes the conversion of two farnesyl pyrophosphates to squalene, an important intermediate in between isoprene and valuable triterpenoids. In this study, we have constructed a novel biosynthesis pathway for squalene in Bacillus subtilis and performed metabolic engineering aiming at facilitating further exploitation and production of squalene-derived triterpenoids. Therefore, systematic studies and analysis were performed including selection of multiple SQS candidates from various organisms, comparison of expression vectors, optimization of cultivation temperatures, and examination of rate-limiting factors within the synthetic pathway. We were, for the first time, able to obtain squalene synthesis in B. subtilis. Furthermore, we achieved a 29-fold increase of squalene yield (0.26–7.5 mg/L) by expressing SQS from Bacillus megaterium and eliminating bottlenecks within the upstream methylerythritol-phosphate pathway. Moreover, our findings showed that also ispA could positively affect the production of squalene." @default.
- W3013782880 created "2020-04-03" @default.
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- W3013782880 date "2020-03-25" @default.
- W3013782880 modified "2023-10-14" @default.
- W3013782880 title "Production of Squalene in <i>Bacillus subtilis</i> by Squalene Synthase Screening and Metabolic Engineering" @default.
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- W3013782880 doi "https://doi.org/10.1021/acs.jafc.0c00375" @default.
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