FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3013844562> ?p ?o ?g. }

• W3013844562 abstract "Abstract Background Foodborne pathogens and their biofilms are considered as one of the most serious problems in human health and food industry. Moreover, safety of foods is a main global concern because of the increasing use of chemical food additives. Ensuring food safety enhances interest in discovery of new alternative compounds such as antimicrobial peptides (AMPs), which can be used as bio-preservatives in the food industry. In this study, the most important antimicrobial peptides of camel milk lactoferrin (lactoferrampin and lactoferricin) were recombinantly expressed in the form of chimeric peptide (cLFchimera) in a food-grade L. lactis strain. P170 expression system was used to express secreted cLFchimera using pAMJ1653 expression vector which harbors a safe (non-antibiotic) selectable marker. Results Peptide purification was carried out using Ni-NTA agarose column from culture medium with concentration of 0.13 mg/mL. The results of disk diffusion test revealed that cLFchimera had considerable antimicrobial activity against a number of major foodborne bacteria. Furthermore, this chimeric peptide showed strong and weak inhibitory effect on biofilm formation against P. aeruginosa , S. aureus E. faecalis, and E. coli, respectively. Antioxidant activity and thermal stability of the chimeric peptide was determined. The results showed that cLFchimera had antioxidant activity (IC 50 : 310 μ/mL) and its activity was not affected after 40 min of boiling. Finally, we evaluated the interaction of the peptide with LPS and DNA in bacteria using molecular dynamic simulation as two main intra and extra cellular targets for AMPs, respectively. Our in silico analysis showed that cLFchimera had strong affinity to both of these targets by positive charged residues after 50 ns molecular dynamic simulation. Conclusions Overall, the engineered food-grade L. lactis generated in the present study successfully expressed a secreted chimeric peptide with antimicrobial properties and could be considered as a promising bio-preservative in the food industry." @default.
• W3013844562 created "2020-04-03" @default.
• W3013844562 creator A5026225963 @default.
• W3013844562 creator A5049922981 @default.
• W3013844562 creator A5066432708 @default.
• W3013844562 creator A5078983560 @default.
• W3013844562 date "2020-03-30" @default.
• W3013844562 modified "2023-10-10" @default.
• W3013844562 title "Generation of an engineered food-grade Lactococcus lactis strain for production of an antimicrobial peptide: in vitro and in silico evaluation" @default.
• W3013844562 cites W1031578623 @default.
• W3013844562 cites W1491824558 @default.
• W3013844562 cites W1607931568 @default.
• W3013844562 cites W1967985926 @default.
• W3013844562 cites W1970294462 @default.
• W3013844562 cites W1972497560 @default.
• W3013844562 cites W1975580333 @default.
• W3013844562 cites W1976673085 @default.
• W3013844562 cites W1978498560 @default.
• W3013844562 cites W1981021420 @default.
• W3013844562 cites W1986191025 @default.
• W3013844562 cites W1987327282 @default.
• W3013844562 cites W1989998611 @default.
• W3013844562 cites W1990603865 @default.
• W3013844562 cites W1998601156 @default.
• W3013844562 cites W2000233490 @default.
• W3013844562 cites W2005410655 @default.
• W3013844562 cites W2006469429 @default.
• W3013844562 cites W2012995574 @default.
• W3013844562 cites W2013065067 @default.
• W3013844562 cites W2017305946 @default.
• W3013844562 cites W2018168557 @default.
• W3013844562 cites W2024063439 @default.
• W3013844562 cites W2024916142 @default.
• W3013844562 cites W2025957152 @default.
• W3013844562 cites W2028392511 @default.
• W3013844562 cites W2028402416 @default.
• W3013844562 cites W2040424528 @default.
• W3013844562 cites W2043983227 @default.
• W3013844562 cites W2044172327 @default.
• W3013844562 cites W2047187675 @default.
• W3013844562 cites W2057315622 @default.
• W3013844562 cites W2057366894 @default.
• W3013844562 cites W2061669178 @default.
• W3013844562 cites W2065853716 @default.
• W3013844562 cites W2070512302 @default.
• W3013844562 cites W2074675295 @default.
• W3013844562 cites W2075703331 @default.
• W3013844562 cites W2080208585 @default.
• W3013844562 cites W2093962378 @default.
• W3013844562 cites W2095894402 @default.
• W3013844562 cites W2108812917 @default.
• W3013844562 cites W2109276669 @default.
• W3013844562 cites W2110778081 @default.
• W3013844562 cites W2121822945 @default.
• W3013844562 cites W2127522551 @default.
• W3013844562 cites W2128635872 @default.
• W3013844562 cites W2131909172 @default.
• W3013844562 cites W2144371848 @default.
• W3013844562 cites W2147183704 @default.
• W3013844562 cites W2148559243 @default.
• W3013844562 cites W2149679556 @default.
• W3013844562 cites W2161762936 @default.
• W3013844562 cites W2163567180 @default.
• W3013844562 cites W2164197975 @default.
• W3013844562 cites W2169644164 @default.
• W3013844562 cites W2171227055 @default.
• W3013844562 cites W2171268876 @default.
• W3013844562 cites W2192685225 @default.
• W3013844562 cites W2315988794 @default.
• W3013844562 cites W2434881634 @default.
• W3013844562 cites W2582965223 @default.
• W3013844562 cites W2794600691 @default.
• W3013844562 cites W2796196791 @default.
• W3013844562 cites W2806002224 @default.
• W3013844562 cites W2891364892 @default.
• W3013844562 cites W2903681279 @default.
• W3013844562 cites W2978958376 @default.
• W3013844562 cites W2988636133 @default.
• W3013844562 cites W3000504779 @default.
• W3013844562 cites W3003290212 @default.
• W3013844562 cites W4293247451 @default.
• W3013844562 cites W586444144 @default.
• W3013844562 doi "https://doi.org/10.1186/s12896-020-00612-3" @default.
• W3013844562 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7106598" @default.
• W3013844562 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32228563" @default.
• W3013844562 hasPublicationYear "2020" @default.
• W3013844562 type Work @default.
• W3013844562 sameAs 3013844562 @default.
• W3013844562 citedByCount "14" @default.
• W3013844562 countsByYear W30138445622020 @default.
• W3013844562 countsByYear W30138445622021 @default.
• W3013844562 countsByYear W30138445622022 @default.
• W3013844562 countsByYear W30138445622023 @default.
• W3013844562 crossrefType "journal-article" @default.
• W3013844562 hasAuthorship W3013844562A5026225963 @default.
• W3013844562 hasAuthorship W3013844562A5049922981 @default.
• W3013844562 hasAuthorship W3013844562A5066432708 @default.
• W3013844562 hasAuthorship W3013844562A5078983560 @default.
• W3013844562 hasBestOaLocation W30138445621 @default.
• W3013844562 hasConcept C104317684 @default.

• next