Matches in SemOpenAlex for { <https://semopenalex.org/work/W3014257462> ?p ?o ?g. }
- W3014257462 abstract "ABSTRACT Hepatitis B virus (HBV) is an enveloped DNA virus that contains a partially double-stranded relaxed circular (rc) DNA. Upon infection, rcDNA is delivered to the nucleus where it is repaired to covalently closed circular (ccc) DNA that serves as the transcription template for all viral RNAs. Our understanding of HBV particle entry dynamics and host pathways regulating intracellular virus trafficking and cccDNA formation is limited. The discovery of sodium taurocholate co-transporting peptide (NTCP) as the primary receptor allows studies on these early steps in viral life cycle. We employed a synchronized infection protocol to quantify HBV entry kinetics. HBV attachment to cells at 4°C is independent of NTCP, however, subsequent particle uptake is NTCP-dependent and reaches saturation at 12h post-infection. HBV uptake is clathrin- and dynamin dependent with actin and tubulin playing a role in the first 6h of infection. Cellular fractionation studies demonstrate HBV DNA in the nucleus within 6h of infection and cccDNA was first detected at 24h post-infection. Our studies show the majority (83%) of cell bound particles enter HepG2-NTCP cells, however, only a minority (<1%) of intracellular rcDNA was converted to cccDNA, highlighting this as a rate-limiting in establishing infection in vitro . This knowledge highlights the deficiencies in our in vitro cell culture systems and will inform the design and evaluation of physiologically relevant models that support efficient HBV replication." @default.
- W3014257462 created "2020-04-10" @default.
- W3014257462 creator A5012231574 @default.
- W3014257462 creator A5028104487 @default.
- W3014257462 creator A5038191788 @default.
- W3014257462 creator A5054796837 @default.
- W3014257462 creator A5056903505 @default.
- W3014257462 creator A5083147198 @default.
- W3014257462 creator A5084389391 @default.
- W3014257462 creator A5091595561 @default.
- W3014257462 date "2020-04-05" @default.
- W3014257462 modified "2023-10-16" @default.
- W3014257462 title "Synchronized infection identifies early rate-limiting steps in the hepatitis B virus life cycle" @default.
- W3014257462 cites W1822042631 @default.
- W3014257462 cites W1838874657 @default.
- W3014257462 cites W1972992218 @default.
- W3014257462 cites W1979009813 @default.
- W3014257462 cites W1983614101 @default.
- W3014257462 cites W1994361603 @default.
- W3014257462 cites W1998671948 @default.
- W3014257462 cites W2002969023 @default.
- W3014257462 cites W2020593605 @default.
- W3014257462 cites W2035655655 @default.
- W3014257462 cites W2073273867 @default.
- W3014257462 cites W2088160623 @default.
- W3014257462 cites W2095077868 @default.
- W3014257462 cites W2107983386 @default.
- W3014257462 cites W2110457185 @default.
- W3014257462 cites W2112032807 @default.
- W3014257462 cites W2113332526 @default.
- W3014257462 cites W2113446109 @default.
- W3014257462 cites W2116812306 @default.
- W3014257462 cites W2117198458 @default.
- W3014257462 cites W2119632652 @default.
- W3014257462 cites W2120341015 @default.
- W3014257462 cites W2120426460 @default.
- W3014257462 cites W2125545448 @default.
- W3014257462 cites W2131134210 @default.
- W3014257462 cites W2138674743 @default.
- W3014257462 cites W2150946670 @default.
- W3014257462 cites W2154120165 @default.
- W3014257462 cites W2154156417 @default.
- W3014257462 cites W2166359678 @default.
- W3014257462 cites W2175117246 @default.
- W3014257462 cites W2177919321 @default.
- W3014257462 cites W2181765919 @default.
- W3014257462 cites W2338985227 @default.
- W3014257462 cites W2347004699 @default.
- W3014257462 cites W2410976255 @default.
- W3014257462 cites W2425890268 @default.
- W3014257462 cites W2471247442 @default.
- W3014257462 cites W2541628542 @default.
- W3014257462 cites W2562446577 @default.
- W3014257462 cites W2565679138 @default.
- W3014257462 cites W2573066704 @default.
- W3014257462 cites W2583077266 @default.
- W3014257462 cites W2588372153 @default.
- W3014257462 cites W2737551710 @default.
- W3014257462 cites W2770860859 @default.
- W3014257462 cites W2772272641 @default.
- W3014257462 cites W2778928474 @default.
- W3014257462 cites W2783694527 @default.
- W3014257462 cites W2794807741 @default.
- W3014257462 cites W2809177606 @default.
- W3014257462 cites W2888073022 @default.
- W3014257462 cites W2888601619 @default.
- W3014257462 cites W2900983194 @default.
- W3014257462 cites W2933300836 @default.
- W3014257462 cites W2938013723 @default.
- W3014257462 cites W2957492237 @default.
- W3014257462 cites W2966033892 @default.
- W3014257462 cites W3005563014 @default.
- W3014257462 cites W3010163084 @default.
- W3014257462 cites W3013536157 @default.
- W3014257462 cites W576512939 @default.
- W3014257462 doi "https://doi.org/10.1101/2020.04.05.026237" @default.
- W3014257462 hasPublicationYear "2020" @default.
- W3014257462 type Work @default.
- W3014257462 sameAs 3014257462 @default.
- W3014257462 citedByCount "1" @default.
- W3014257462 countsByYear W30142574622020 @default.
- W3014257462 crossrefType "posted-content" @default.
- W3014257462 hasAuthorship W3014257462A5012231574 @default.
- W3014257462 hasAuthorship W3014257462A5028104487 @default.
- W3014257462 hasAuthorship W3014257462A5038191788 @default.
- W3014257462 hasAuthorship W3014257462A5054796837 @default.
- W3014257462 hasAuthorship W3014257462A5056903505 @default.
- W3014257462 hasAuthorship W3014257462A5083147198 @default.
- W3014257462 hasAuthorship W3014257462A5084389391 @default.
- W3014257462 hasAuthorship W3014257462A5091595561 @default.
- W3014257462 hasBestOaLocation W30142574621 @default.
- W3014257462 hasConcept C140704245 @default.
- W3014257462 hasConcept C159047783 @default.
- W3014257462 hasConcept C189490182 @default.
- W3014257462 hasConcept C194575299 @default.
- W3014257462 hasConcept C2522874641 @default.
- W3014257462 hasConcept C25897203 @default.
- W3014257462 hasConcept C2777410769 @default.
- W3014257462 hasConcept C2780593183 @default.
- W3014257462 hasConcept C54355233 @default.