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• W3014421123 abstract "Abstract Background Smad proteins are essential cellular mediators within the transforming growth factor-β (TGF-β) superfamily. They directly transmit incoming signals from the cell surface receptors to the nucleus. In spite of their functional importance, almost nothing is known about Smad proteins in parasitic nematodes including Haemonchus contortus , an important blood-sucking nematode of small ruminants. Methods Based on genomic and transcriptome data for H. contortus and using bioinformatics methods, a Smad homologue (called Hco - daf-8 ) was inferred from H . contortus and the structural characteristics of this gene and its encoded protein Hco -DAF-8 established. Using real-time PCR and immunofluorescence assays, temporal transcriptional and spatial expression profiles of Hco - daf-8 were studied. Gene rescue in Caenorhabditis elegans was then applied to assess the function of Hco-daf-8 and a specific inhibitor of human Smad3 (called SIS3) was employed to evaluate the roles of Hco -DAF-8 in H. contortus development. Results The features of Hco- DAF-8 (502 amino acids), including conserved R-Smad domains and residues of the L3-loop that determine pathway specificity, are consistent with a TGF-β type I receptor-activated R-Smad. The Hco-daf-8 gene was transcribed in all developmental stages of H. contortus studied, with a higher level of transcription in the fourth-stage larval (L4) females and the highest level in adult males. Hco -DAF-8 was expressed in the platymyarian muscular cells, intestine and reproductive system of adult stages. Gene rescue experiments showed that Hco-daf-8 was able to partially rescue gene function in a daf-8 deficient mutant strain of C. elegans , leading to a resumption of normal development. In H. contortus , SIS3 was shown to affect H. contortus development from the exsheathed third-stage larvae (L3s) to L4s in vitro . Conclusions These findings suggest that Hco -DAF-8, encoded by the gene Hco-daf-8 , is an important cellular mediator of H. contortus development via the TGF-β signalling pathway. They provide a basis for future explorations of Hco -DAF-8 and associated pathways in H. contortus and other important parasitic nematodes." @default.
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• W3014421123 date "2020-04-03" @default.
• W3014421123 modified "2023-09-26" @default.
• W3014421123 title "Identification and characterization of an R-Smad homologue (Hco-DAF-8) from Haemonchus contortus" @default.
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• W3014421123 doi "https://doi.org/10.1186/s13071-020-04034-0" @default.
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