FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3014498889> ?p ?o ?g. }

• W3014498889 endingPage "391" @default.
• W3014498889 startingPage "391" @default.
• W3014498889 abstract "A highly virulent porcine epidemic diarrhea virus (PEDV) appeared in China and spread rapidly to neighbor countries, which have led to great economic losses to the pig industry. In the present study, we isolated a PEDV using Vero cells and serially propagated 100 passages. PEDV SDSX16 was characterized in vitro and in vivo. The viral titers increased to 107.6 TCID50/mL (100th) by serial passages. The spike (S) gene and the whole gene of the SDSX16 virus was fully sequenced to assess the genetic stability and relatedness to previously identified PEDV. Along with successive passage in vitro, there were 18 nucleotides (nt) deletion occurred in the spike (S) gene resulting in a deletion of six amino acids when the SDSX16 strain was passaged to the 64th generation, and this deletion was stable until the P100. However, the ORF1a/b, M, N, E, and ORF3 genes had only a few point mutations in amino acids and no deletions. According to growth kinetics experiments, the SDSX16 deletion strain significantly enhanced its replication in Vero cells since it was passaged to the 64th generation. The animal studies showed that PEDV SDSX16-P10 caused more severe diarrhea and vomiting, fecal shedding, and acute atrophic enteritis than SDSX16-P75, indicating that SDSX16-P10 is enteropathogenic in the natural host, and the pathogenicity of SDSX16 decreased with successive passage in vitro. However, SDSX16-P10 was found to cause lower levels of cytokine expression than SDSX16-P75 using real-time PCR and flow cytometry, such as IL1β, IL6, IFN-β, TNF-α, indicating that SDSX16-P10 might inhibit the expression of cytokines. Our data indicated that successive passage in vitro resulted in virulent attenuation in vivo of the PEDV variant strain SDSX16." @default.
• W3014498889 created "2020-04-10" @default.
• W3014498889 creator A5000797820 @default.
• W3014498889 creator A5005426960 @default.
• W3014498889 creator A5012379008 @default.
• W3014498889 creator A5016161951 @default.
• W3014498889 creator A5019367920 @default.
• W3014498889 creator A5022640967 @default.
• W3014498889 creator A5025652870 @default.
• W3014498889 creator A5035743221 @default.
• W3014498889 creator A5049903336 @default.
• W3014498889 creator A5058787206 @default.
• W3014498889 creator A5080247586 @default.
• W3014498889 creator A5085102532 @default.
• W3014498889 creator A5087018671 @default.
• W3014498889 date "2020-04-01" @default.
• W3014498889 modified "2023-10-10" @default.
• W3014498889 title "Successive Passage In Vitro Led to Lower Virulence and Higher Titer of A Variant Porcine Epidemic Diarrhea Virus" @default.
• W3014498889 cites W1978950020 @default.
• W3014498889 cites W1979426398 @default.
• W3014498889 cites W1986361699 @default.
• W3014498889 cites W1991879923 @default.
• W3014498889 cites W1992379328 @default.
• W3014498889 cites W1992810738 @default.
• W3014498889 cites W1998827160 @default.
• W3014498889 cites W2001925977 @default.
• W3014498889 cites W2012504769 @default.
• W3014498889 cites W2035494209 @default.
• W3014498889 cites W2042433863 @default.
• W3014498889 cites W2070315095 @default.
• W3014498889 cites W2075464488 @default.
• W3014498889 cites W2086955313 @default.
• W3014498889 cites W2089712266 @default.
• W3014498889 cites W2094023848 @default.
• W3014498889 cites W2096372976 @default.
• W3014498889 cites W2097957152 @default.
• W3014498889 cites W2098675796 @default.
• W3014498889 cites W2099450221 @default.
• W3014498889 cites W2099480987 @default.
• W3014498889 cites W2100239778 @default.
• W3014498889 cites W2102974062 @default.
• W3014498889 cites W2106713698 @default.
• W3014498889 cites W2107513655 @default.
• W3014498889 cites W2132784079 @default.
• W3014498889 cites W2135248979 @default.
• W3014498889 cites W2148270110 @default.
• W3014498889 cites W2156151768 @default.
• W3014498889 cites W2166880388 @default.
• W3014498889 cites W2169843095 @default.
• W3014498889 cites W2172178713 @default.
• W3014498889 cites W2177196194 @default.
• W3014498889 cites W2233577420 @default.
• W3014498889 cites W2256756668 @default.
• W3014498889 cites W2342205747 @default.
• W3014498889 cites W2400382315 @default.
• W3014498889 cites W2513256790 @default.
• W3014498889 cites W2557370976 @default.
• W3014498889 cites W2579751664 @default.
• W3014498889 cites W2735084243 @default.
• W3014498889 cites W2894950287 @default.
• W3014498889 cites W2911632907 @default.
• W3014498889 cites W2915017383 @default.
• W3014498889 cites W2916300783 @default.
• W3014498889 cites W2957510770 @default.
• W3014498889 cites W2992675192 @default.
• W3014498889 cites W4248552215 @default.
• W3014498889 cites W879986050 @default.
• W3014498889 doi "https://doi.org/10.3390/v12040391" @default.
• W3014498889 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7232491" @default.
• W3014498889 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32244640" @default.
• W3014498889 hasPublicationYear "2020" @default.
• W3014498889 type Work @default.
• W3014498889 sameAs 3014498889 @default.
• W3014498889 citedByCount "5" @default.
• W3014498889 countsByYear W30144988892020 @default.
• W3014498889 countsByYear W30144988892021 @default.
• W3014498889 countsByYear W30144988892022 @default.
• W3014498889 crossrefType "journal-article" @default.
• W3014498889 hasAuthorship W3014498889A5000797820 @default.
• W3014498889 hasAuthorship W3014498889A5005426960 @default.
• W3014498889 hasAuthorship W3014498889A5012379008 @default.
• W3014498889 hasAuthorship W3014498889A5016161951 @default.
• W3014498889 hasAuthorship W3014498889A5019367920 @default.
• W3014498889 hasAuthorship W3014498889A5022640967 @default.
• W3014498889 hasAuthorship W3014498889A5025652870 @default.
• W3014498889 hasAuthorship W3014498889A5035743221 @default.
• W3014498889 hasAuthorship W3014498889A5049903336 @default.
• W3014498889 hasAuthorship W3014498889A5058787206 @default.
• W3014498889 hasAuthorship W3014498889A5080247586 @default.
• W3014498889 hasAuthorship W3014498889A5085102532 @default.
• W3014498889 hasAuthorship W3014498889A5087018671 @default.
• W3014498889 hasBestOaLocation W30144988891 @default.
• W3014498889 hasConcept C104317684 @default.
• W3014498889 hasConcept C126322002 @default.
• W3014498889 hasConcept C132351222 @default.
• W3014498889 hasConcept C159047783 @default.
• W3014498889 hasConcept C202751555 @default.
• W3014498889 hasConcept C2522874641 @default.

• next