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• W3014768421 endingPage "1091" @default.
• W3014768421 startingPage "1082" @default.
• W3014768421 abstract "Loop-mediated isothermal amplification (LAMP) is a sensitive and widely used gene amplification technique. However, high amplification efficiency and amplification products containing multiple inverted repeats make the LAMP reaction extremely vulnerable to false-positive amplification caused by contamination. Herein, a contamination-free LAMP (CUT-LAMP) assisted by the CRISPR/Cas9 cleavage with superior reliability and durability has been reported. The core of CUT-LAMP is the engineering of the forward or backward inner primer in the target-independent region, which makes the LAMP products contain a protospacer adjacent motif (PAM) site for the CRISPR/Cas9 recognition. For the CUT-LAMP reaction, cross-contamination can be efficiently cleaved by the corresponding Cas9/sgRNA, but the target gene can get rid of digestion due to the lack of a PAM site near the recognition region. CUT-LAMP shows impressive contamination resistance but does not significantly increase procedure complexity; thus, it represents a simple and versatile toolkit facilitating the adoption by open- and closed-tube detection format." @default.
• W3014768421 created "2020-04-10" @default.
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• W3014768421 date "2020-04-03" @default.
• W3014768421 modified "2023-10-14" @default.
• W3014768421 title "CUT-LAMP: Contamination-Free Loop-Mediated Isothermal Amplification Based on the CRISPR/Cas9 Cleavage" @default.
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• W3014768421 doi "https://doi.org/10.1021/acssensors.0c00034" @default.
• W3014768421 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32242409" @default.
• W3014768421 hasPublicationYear "2020" @default.
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