Matches in SemOpenAlex for { <https://semopenalex.org/work/W3014844199> ?p ?o ?g. }
- W3014844199 abstract "Abstract In bacterial systems, CRISPR-Cas transcriptional activation (CRISPRa) has the potential to dramatically expand our ability to regulate gene expression, but we lack predictive rules for designing effective gRNA target sites. Here, we identify multiple features of bacterial promoters that impose stringent requirements on CRISPRa target sites. Notably, we observe narrow, 2–4 base windows of effective sites with a periodicity corresponding to one helical turn of DNA, spanning ~40 bases and centered ~80 bases upstream of the TSS. However, we also identify two features suggesting the potential for broad scope: CRISPRa is effective at a broad range of σ 70 -family promoters, and an expanded PAM dCas9 allows the activation of promoters that cannot be activated by S. pyogenes dCas9. These results provide a roadmap for future engineering efforts to further expand and generalize the scope of bacterial CRISPRa." @default.
- W3014844199 created "2020-04-10" @default.
- W3014844199 creator A5009734051 @default.
- W3014844199 creator A5027029742 @default.
- W3014844199 creator A5031317822 @default.
- W3014844199 creator A5046996365 @default.
- W3014844199 creator A5057198078 @default.
- W3014844199 creator A5081699843 @default.
- W3014844199 creator A5084834508 @default.
- W3014844199 date "2020-04-01" @default.
- W3014844199 modified "2023-10-07" @default.
- W3014844199 title "Effective CRISPRa-mediated control of gene expression in bacteria must overcome strict target site requirements" @default.
- W3014844199 cites W1884201982 @default.
- W3014844199 cites W1967676653 @default.
- W3014844199 cites W1971696785 @default.
- W3014844199 cites W1971729222 @default.
- W3014844199 cites W1995514232 @default.
- W3014844199 cites W1996450159 @default.
- W3014844199 cites W2023401038 @default.
- W3014844199 cites W2026767056 @default.
- W3014844199 cites W2038186289 @default.
- W3014844199 cites W2041025550 @default.
- W3014844199 cites W2042922327 @default.
- W3014844199 cites W2051048419 @default.
- W3014844199 cites W2052945757 @default.
- W3014844199 cites W2067135569 @default.
- W3014844199 cites W2080413755 @default.
- W3014844199 cites W2090660721 @default.
- W3014844199 cites W2105599696 @default.
- W3014844199 cites W2107277218 @default.
- W3014844199 cites W2107523606 @default.
- W3014844199 cites W2113643296 @default.
- W3014844199 cites W2118464978 @default.
- W3014844199 cites W2119815359 @default.
- W3014844199 cites W2120932131 @default.
- W3014844199 cites W2127862779 @default.
- W3014844199 cites W2140841500 @default.
- W3014844199 cites W2144486838 @default.
- W3014844199 cites W2147057508 @default.
- W3014844199 cites W2152505144 @default.
- W3014844199 cites W2160051211 @default.
- W3014844199 cites W2163173465 @default.
- W3014844199 cites W2182456467 @default.
- W3014844199 cites W2293809279 @default.
- W3014844199 cites W2400266213 @default.
- W3014844199 cites W2400411104 @default.
- W3014844199 cites W2558268315 @default.
- W3014844199 cites W2560707317 @default.
- W3014844199 cites W2580171608 @default.
- W3014844199 cites W2618047787 @default.
- W3014844199 cites W2793309516 @default.
- W3014844199 cites W2808828797 @default.
- W3014844199 cites W2952170484 @default.
- W3014844199 cites W2969515168 @default.
- W3014844199 doi "https://doi.org/10.1038/s41467-020-15454-y" @default.
- W3014844199 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7113249" @default.
- W3014844199 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32238808" @default.
- W3014844199 hasPublicationYear "2020" @default.
- W3014844199 type Work @default.
- W3014844199 sameAs 3014844199 @default.
- W3014844199 citedByCount "57" @default.
- W3014844199 countsByYear W30148441992020 @default.
- W3014844199 countsByYear W30148441992021 @default.
- W3014844199 countsByYear W30148441992022 @default.
- W3014844199 countsByYear W30148441992023 @default.
- W3014844199 crossrefType "journal-article" @default.
- W3014844199 hasAuthorship W3014844199A5009734051 @default.
- W3014844199 hasAuthorship W3014844199A5027029742 @default.
- W3014844199 hasAuthorship W3014844199A5031317822 @default.
- W3014844199 hasAuthorship W3014844199A5046996365 @default.
- W3014844199 hasAuthorship W3014844199A5057198078 @default.
- W3014844199 hasAuthorship W3014844199A5081699843 @default.
- W3014844199 hasAuthorship W3014844199A5084834508 @default.
- W3014844199 hasBestOaLocation W30148441991 @default.
- W3014844199 hasConcept C101762097 @default.
- W3014844199 hasConcept C104317684 @default.
- W3014844199 hasConcept C132455925 @default.
- W3014844199 hasConcept C150194340 @default.
- W3014844199 hasConcept C199360897 @default.
- W3014844199 hasConcept C2778012447 @default.
- W3014844199 hasConcept C41008148 @default.
- W3014844199 hasConcept C54355233 @default.
- W3014844199 hasConcept C552990157 @default.
- W3014844199 hasConcept C70721500 @default.
- W3014844199 hasConcept C86803240 @default.
- W3014844199 hasConcept C98108389 @default.
- W3014844199 hasConceptScore W3014844199C101762097 @default.
- W3014844199 hasConceptScore W3014844199C104317684 @default.
- W3014844199 hasConceptScore W3014844199C132455925 @default.
- W3014844199 hasConceptScore W3014844199C150194340 @default.
- W3014844199 hasConceptScore W3014844199C199360897 @default.
- W3014844199 hasConceptScore W3014844199C2778012447 @default.
- W3014844199 hasConceptScore W3014844199C41008148 @default.
- W3014844199 hasConceptScore W3014844199C54355233 @default.
- W3014844199 hasConceptScore W3014844199C552990157 @default.
- W3014844199 hasConceptScore W3014844199C70721500 @default.
- W3014844199 hasConceptScore W3014844199C86803240 @default.
- W3014844199 hasConceptScore W3014844199C98108389 @default.
- W3014844199 hasIssue "1" @default.
- W3014844199 hasLocation W30148441991 @default.