Matches in SemOpenAlex for { <https://semopenalex.org/work/W3015275035> ?p ?o ?g. }
- W3015275035 abstract "We present a single-objective light-sheet microscope, also known as an oblique-plane microscope, that uses a bespoke glass-tipped tertiary objective and improves the resolution, field of view, usability, and stability over previous variants. Owing to its high numerical aperture optics, this microscope achieves the highest lateral resolution in light-sheet fluorescence microscopy, and its axial resolution is similar to that of Lattice Light-Sheet Microscopy. Given this performance, we demonstrate high-resolution imaging of clathrin-mediated endocytosis, vimentin, the endoplasmic reticulum, membrane dynamics, and natural killer cell-mediated cell death. Furthermore, we image biological phenomena that would be otherwise challenging or impossible to perform in a traditional light-sheet microscope geometry, including cell migration through a confined space within a microfluidic device, photoactivation of PI3K, and diffusion of cytoplasmic rheological tracers at a volumetric rate of 14 Hz." @default.
- W3015275035 created "2020-04-17" @default.
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- W3015275035 date "2020-04-08" @default.
- W3015275035 modified "2023-09-26" @default.
- W3015275035 title "A single-objective light-sheet microscope with 200 nm-scale resolution" @default.
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