FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3015529423> ?p ?o ?g. }

• W3015529423 abstract "Abstract The PCR amplification of majority of the ssRNA of both genomic and non-genomic mRNA is accomplished by RT-PCR. The mRNA is subjected to cDNA synthesis using reverse transcriptase and either Oligo(dT)18, or random or gene specific reverse primers based priming strategies. The choice of primer largely depends on the nature of 3 prime terminus of mRNA and length of cDNA synthesized. In general, oligo(dT)18 is the preferred choice for mRNAs having poly(A) tail at 3 prime terminus. In general, tobamoviruses lack any poly(A) tail at their 3 prime untranslated region (UTR) which forms a tRNA like structure and upstream pseudoknot domain except tow viruses viz., Hibiscus latent Fort Pierce virus (HLFPV) and Hibiscus latent Singapore virus (HLSV) which accommodate internal poly(A) sequences of 46 and 87 nucleotides long, respectively in their 3 prime UTR. However, determination of full nucleotide sequence of Pepper mild mottle virus (PMMoV) using an oligo(dT)18 primed cDNA as template indicated the libertinism of oligo(dT)18 in priming cDNA synthesis of RNA template which are known to lack poly(A) tail. at the end or internally at its 3 prime end. Moreover, coat protein (CP) gene of PMMoV and bean common mosaic virus (BCMV) (Potyvirus with a poly(A) tract at its 3 prime end) was amplified using cDNA primed with random primer as well as oligo(dT)18 was successfully amplified but with significant variation in the intensity of the amplification band in case of PMMoV but not in BCMV. This clearly indicated the presence of PMMoV mRNA with polyadenylated 3 prime tail in total RNA isolated from PMMoV infected capsicum leaves with abundance of non-polyadenylated PMMoV genomic RNA (gRNA). Hence, we hypothesize that the generation of polyadenylated RNA population during the infection cycle of PMMoV in pepper may be possible reason for libertinism of oligo(dT)18 in priming cDNA synthesis of RNA template isolated from PMMoV infected leaves followed by amplification of entire PMMoV genome through RT-PCR. This is first study indicating the presence of polyadenylated or polyadenylated rich regions in PMMoV gRNA acquired during the infection cycle in pepper." @default.
• W3015529423 created "2020-04-17" @default.
• W3015529423 creator A5016877462 @default.
• W3015529423 creator A5038073928 @default.
• W3015529423 creator A5053288344 @default.
• W3015529423 creator A5057793316 @default.
• W3015529423 date "2020-04-13" @default.
• W3015529423 modified "2023-10-16" @default.
• W3015529423 title "Presence of polyadenylated 3’ tail in RNA ofPepper mild mottle virusallows the Oligo(dT)18in Priming cDNA Synthesis of its genomic RNA template" @default.
• W3015529423 cites W1480192010 @default.
• W3015529423 cites W1503351543 @default.
• W3015529423 cites W1519900379 @default.
• W3015529423 cites W1537179103 @default.
• W3015529423 cites W197305525 @default.
• W3015529423 cites W1974434328 @default.
• W3015529423 cites W2008934800 @default.
• W3015529423 cites W2019494484 @default.
• W3015529423 cites W2031839739 @default.
• W3015529423 cites W2039915263 @default.
• W3015529423 cites W2042659295 @default.
• W3015529423 cites W2044534227 @default.
• W3015529423 cites W2047227959 @default.
• W3015529423 cites W2060470132 @default.
• W3015529423 cites W2060916307 @default.
• W3015529423 cites W2063443647 @default.
• W3015529423 cites W2063514224 @default.
• W3015529423 cites W2070586753 @default.
• W3015529423 cites W2118421952 @default.
• W3015529423 cites W2118764989 @default.
• W3015529423 cites W2122011206 @default.
• W3015529423 cites W2130086998 @default.
• W3015529423 cites W2145665475 @default.
• W3015529423 cites W2155380737 @default.
• W3015529423 cites W2334623780 @default.
• W3015529423 cites W2963907827 @default.
• W3015529423 cites W312574127 @default.
• W3015529423 doi "https://doi.org/10.1101/2020.04.13.039180" @default.
• W3015529423 hasPublicationYear "2020" @default.
• W3015529423 type Work @default.
• W3015529423 sameAs 3015529423 @default.
• W3015529423 citedByCount "0" @default.
• W3015529423 crossrefType "posted-content" @default.
• W3015529423 hasAuthorship W3015529423A5016877462 @default.
• W3015529423 hasAuthorship W3015529423A5038073928 @default.
• W3015529423 hasAuthorship W3015529423A5053288344 @default.
• W3015529423 hasAuthorship W3015529423A5057793316 @default.
• W3015529423 hasBestOaLocation W30155294231 @default.
• W3015529423 hasConcept C104317684 @default.
• W3015529423 hasConcept C105130999 @default.
• W3015529423 hasConcept C142575336 @default.
• W3015529423 hasConcept C153911025 @default.
• W3015529423 hasConcept C156719811 @default.
• W3015529423 hasConcept C159047783 @default.
• W3015529423 hasConcept C178790620 @default.
• W3015529423 hasConcept C185592680 @default.
• W3015529423 hasConcept C187882448 @default.
• W3015529423 hasConcept C19924922 @default.
• W3015529423 hasConcept C2777563447 @default.
• W3015529423 hasConcept C2779535198 @default.
• W3015529423 hasConcept C41258723 @default.
• W3015529423 hasConcept C50724178 @default.
• W3015529423 hasConcept C54355233 @default.
• W3015529423 hasConcept C67705224 @default.
• W3015529423 hasConcept C86803240 @default.
• W3015529423 hasConcept C89604277 @default.
• W3015529423 hasConceptScore W3015529423C104317684 @default.
• W3015529423 hasConceptScore W3015529423C105130999 @default.
• W3015529423 hasConceptScore W3015529423C142575336 @default.
• W3015529423 hasConceptScore W3015529423C153911025 @default.
• W3015529423 hasConceptScore W3015529423C156719811 @default.
• W3015529423 hasConceptScore W3015529423C159047783 @default.
• W3015529423 hasConceptScore W3015529423C178790620 @default.
• W3015529423 hasConceptScore W3015529423C185592680 @default.
• W3015529423 hasConceptScore W3015529423C187882448 @default.
• W3015529423 hasConceptScore W3015529423C19924922 @default.
• W3015529423 hasConceptScore W3015529423C2777563447 @default.
• W3015529423 hasConceptScore W3015529423C2779535198 @default.
• W3015529423 hasConceptScore W3015529423C41258723 @default.
• W3015529423 hasConceptScore W3015529423C50724178 @default.
• W3015529423 hasConceptScore W3015529423C54355233 @default.
• W3015529423 hasConceptScore W3015529423C67705224 @default.
• W3015529423 hasConceptScore W3015529423C86803240 @default.
• W3015529423 hasConceptScore W3015529423C89604277 @default.
• W3015529423 hasLocation W30155294231 @default.
• W3015529423 hasOpenAccess W3015529423 @default.
• W3015529423 hasPrimaryLocation W30155294231 @default.
• W3015529423 hasRelatedWork W10777826 @default.
• W3015529423 hasRelatedWork W1585835 @default.
• W3015529423 hasRelatedWork W16658660 @default.
• W3015529423 hasRelatedWork W2676309 @default.
• W3015529423 hasRelatedWork W32425459 @default.
• W3015529423 hasRelatedWork W36295273 @default.
• W3015529423 hasRelatedWork W40032362 @default.
• W3015529423 hasRelatedWork W4504755 @default.
• W3015529423 hasRelatedWork W623765 @default.
• W3015529423 hasRelatedWork W7247056 @default.
• W3015529423 isParatext "false" @default.
• W3015529423 isRetracted "false" @default.
• W3015529423 magId "3015529423" @default.
• W3015529423 workType "article" @default.