FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3015532035> ?p ?o ?g. }

• W3015532035 abstract "Porphyromonas gingivalis, the main etiologic agent of periodontitis, secretes cysteine proteases named gingipains. HRgpA and RgpB gingipains have Arg-specificity, while Kgp gingipain is Lys-specific. Together they can cleave an array of proteins and importantly contribute to the development of periodontitis. In this study we focused on gingipain-exerted proteolysis at the cell surface of human gingival epithelial cells (telomerase immortalized gingival keratinocytes [TIGK]) in order to better understand the molecular mechanisms behind tissue destruction in periodontitis. Using mass spectrometry, we investigated the whole sheddome/degradome of TIGK cell surface proteins by P. gingivalis strains differing in gingipain expression and by purified gingipains, and performed the first global proteomic analysis of gingipain membrane proteolysis. Incubation of TIGK cells with P. gingivalis resulted in massive degradation of proteins already at low multiplicity of infection, whereas incubating cells with purified gingipains resulted in more discrete patterns, indicative of a combination of complete degradation and shedding of membrane proteins. Most of the identified gingipain substrates were molecules involved in adhesion, suggesting that gingipains may cause tissue damage through cleavage of cell contacts, resulting in cell detachment and rounding, and consequently leading to anoikis. However, HRgpA and RgpB gingipains differ in their mechanism of action. While RgpB rapidly degraded the proteins, HRgpA exhibited a much slower proteolysis indicative of ectodomain shedding, as demonstrated for the transferrin receptor protein 1 (TFRC). These results reveal a molecular underpinning to P. gingivalis-induced tissue destruction and enhance our knowledge of the role of P. gingivalis proteases in the pathobiology of periodontitis. Proteomics data are available via ProteomeXchange with identifier PXD015679." @default.
• W3015532035 created "2020-04-17" @default.
• W3015532035 creator A5002467317 @default.
• W3015532035 creator A5008946134 @default.
• W3015532035 creator A5011791950 @default.
• W3015532035 creator A5021770886 @default.
• W3015532035 creator A5034158864 @default.
• W3015532035 creator A5036463770 @default.
• W3015532035 creator A5046721804 @default.
• W3015532035 creator A5056440307 @default.
• W3015532035 creator A5081481754 @default.
• W3015532035 date "2020-04-28" @default.
• W3015532035 modified "2023-10-16" @default.
• W3015532035 title "Proteolysis of Gingival Keratinocyte Cell Surface Proteins by Gingipains Secreted From Porphyromonas gingivalis – Proteomic Insights Into Mechanisms Behind Tissue Damage in the Diseased Gingiva" @default.
• W3015532035 cites W1514298611 @default.
• W3015532035 cites W1672688094 @default.
• W3015532035 cites W1691524838 @default.
• W3015532035 cites W1827457186 @default.
• W3015532035 cites W1895718854 @default.
• W3015532035 cites W1971558491 @default.
• W3015532035 cites W1974224407 @default.
• W3015532035 cites W1980671328 @default.
• W3015532035 cites W1986656413 @default.
• W3015532035 cites W1990025846 @default.
• W3015532035 cites W1994214832 @default.
• W3015532035 cites W1997504014 @default.
• W3015532035 cites W2002553683 @default.
• W3015532035 cites W2005882400 @default.
• W3015532035 cites W2010368907 @default.
• W3015532035 cites W2013322021 @default.
• W3015532035 cites W2015027028 @default.
• W3015532035 cites W2016353911 @default.
• W3015532035 cites W2021838232 @default.
• W3015532035 cites W2029303574 @default.
• W3015532035 cites W2030853726 @default.
• W3015532035 cites W2031861761 @default.
• W3015532035 cites W2032625696 @default.
• W3015532035 cites W2035602414 @default.
• W3015532035 cites W2036321220 @default.
• W3015532035 cites W2043251838 @default.
• W3015532035 cites W2046195724 @default.
• W3015532035 cites W2047128020 @default.
• W3015532035 cites W2053008139 @default.
• W3015532035 cites W2054366592 @default.
• W3015532035 cites W2056077453 @default.
• W3015532035 cites W2057288160 @default.
• W3015532035 cites W2058633956 @default.
• W3015532035 cites W2062429356 @default.
• W3015532035 cites W2063546126 @default.
• W3015532035 cites W2072471109 @default.
• W3015532035 cites W2072581123 @default.
• W3015532035 cites W2073919816 @default.
• W3015532035 cites W2080752012 @default.
• W3015532035 cites W2084544627 @default.
• W3015532035 cites W2094274661 @default.
• W3015532035 cites W2098058255 @default.
• W3015532035 cites W2098830851 @default.
• W3015532035 cites W2099013880 @default.
• W3015532035 cites W2102117283 @default.
• W3015532035 cites W2107177655 @default.
• W3015532035 cites W2110063674 @default.
• W3015532035 cites W2113100474 @default.
• W3015532035 cites W2124954099 @default.
• W3015532035 cites W2127993259 @default.
• W3015532035 cites W2131427209 @default.
• W3015532035 cites W2133664523 @default.
• W3015532035 cites W2134444754 @default.
• W3015532035 cites W2135800998 @default.
• W3015532035 cites W2138452485 @default.
• W3015532035 cites W2145139438 @default.
• W3015532035 cites W2155169733 @default.
• W3015532035 cites W2156806286 @default.
• W3015532035 cites W2164461702 @default.
• W3015532035 cites W2172100391 @default.
• W3015532035 cites W2275143806 @default.
• W3015532035 cites W2565176913 @default.
• W3015532035 cites W2755744237 @default.
• W3015532035 cites W2870417857 @default.
• W3015532035 cites W2883839079 @default.
• W3015532035 cites W2899760200 @default.
• W3015532035 cites W2918086501 @default.
• W3015532035 cites W4241861083 @default.
• W3015532035 doi "https://doi.org/10.3389/fmicb.2020.00722" @default.
• W3015532035 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7198712" @default.
• W3015532035 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32411104" @default.
• W3015532035 hasPublicationYear "2020" @default.
• W3015532035 type Work @default.
• W3015532035 sameAs 3015532035 @default.
• W3015532035 citedByCount "11" @default.
• W3015532035 countsByYear W30155320352021 @default.
• W3015532035 countsByYear W30155320352022 @default.
• W3015532035 countsByYear W30155320352023 @default.
• W3015532035 crossrefType "journal-article" @default.
• W3015532035 hasAuthorship W3015532035A5002467317 @default.
• W3015532035 hasAuthorship W3015532035A5008946134 @default.
• W3015532035 hasAuthorship W3015532035A5011791950 @default.
• W3015532035 hasAuthorship W3015532035A5021770886 @default.
• W3015532035 hasAuthorship W3015532035A5034158864 @default.
• W3015532035 hasAuthorship W3015532035A5036463770 @default.
• W3015532035 hasAuthorship W3015532035A5046721804 @default.

• next