FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3015853029> ?p ?o ?g. }

• W3015853029 endingPage "7248" @default.
• W3015853029 startingPage "7235" @default.
• W3015853029 abstract "The mitochondrion of malaria parasites contains several clinically validated drug targets. Within Plasmodium spp., the causative agents of malaria, the mitochondrial DNA (mtDNA) is only 6 kb long, being the smallest mitochondrial genome among all eukaryotes. The mtDNA encodes only three proteins of the mitochondrial electron transport chain and ∼27 small, fragmented rRNA genes having lengths of 22-195 nucleotides. The rRNA fragments are thought to form a mitochondrial ribosome (mitoribosome), together with ribosomal proteins imported from the cytosol. The mitoribosome of Plasmodium falciparum is essential for maintenance of the mitochondrial membrane potential and parasite viability. However, the role of the mitoribosome in sustaining the metabolic status of the parasite mitochondrion remains unclear. The small ribosomal subunit in P. falciparum has 14 annotated mitoribosomal proteins, and employing a CRISPR/Cas9-based conditional knockdown tool, here we verified the location and tested the essentiality of three candidates (PfmtRPS12, PfmtRPS17, and PfmtRPS18). Using immuno-EM, we provide evidence that the P. falciparum mitoribosome is closely associated with the mitochondrial inner membrane. Upon knockdown of the mitoribosome, parasites became hypersensitive to inhibitors targeting mitochondrial Complex III (bc1), dihydroorotate dehydrogenase (DHOD), and the F1F0-ATP synthase complex. Furthermore, the mitoribosome knockdown blocked the pyrimidine biosynthesis pathway and reduced the cellular pool of pyrimidine nucleotides. These results suggest that disruption of the P. falciparum mitoribosome compromises the metabolic capacity of the mitochondrion, rendering the parasite hypersensitive to a panel of inhibitors that target mitochondrial functions." @default.
• W3015853029 created "2020-04-17" @default.
• W3015853029 creator A5012463920 @default.
• W3015853029 creator A5023306273 @default.
• W3015853029 creator A5024961418 @default.
• W3015853029 creator A5027004504 @default.
• W3015853029 creator A5032273314 @default.
• W3015853029 creator A5036379634 @default.
• W3015853029 creator A5061317559 @default.
• W3015853029 creator A5068725892 @default.
• W3015853029 creator A5090802023 @default.
• W3015853029 date "2020-05-01" @default.
• W3015853029 modified "2023-10-18" @default.
• W3015853029 title "Genetic ablation of the mitoribosome in the malaria parasite Plasmodium falciparum sensitizes it to antimalarials that target mitochondrial functions" @default.
• W3015853029 cites W1527916516 @default.
• W3015853029 cites W1829280477 @default.
• W3015853029 cites W1964333133 @default.
• W3015853029 cites W1968408636 @default.
• W3015853029 cites W1970465318 @default.
• W3015853029 cites W1976224242 @default.
• W3015853029 cites W1976977157 @default.
• W3015853029 cites W1977123265 @default.
• W3015853029 cites W1996993889 @default.
• W3015853029 cites W2006035995 @default.
• W3015853029 cites W2007561244 @default.
• W3015853029 cites W2007852671 @default.
• W3015853029 cites W2010332067 @default.
• W3015853029 cites W2017123927 @default.
• W3015853029 cites W2019851585 @default.
• W3015853029 cites W2021861406 @default.
• W3015853029 cites W2025605230 @default.
• W3015853029 cites W2040246647 @default.
• W3015853029 cites W2040581144 @default.
• W3015853029 cites W2043631207 @default.
• W3015853029 cites W2046194878 @default.
• W3015853029 cites W2049009393 @default.
• W3015853029 cites W2058059796 @default.
• W3015853029 cites W2067134743 @default.
• W3015853029 cites W2068427957 @default.
• W3015853029 cites W2083157472 @default.
• W3015853029 cites W2089337157 @default.
• W3015853029 cites W2095745314 @default.
• W3015853029 cites W2103283939 @default.
• W3015853029 cites W2110343132 @default.
• W3015853029 cites W2112050041 @default.
• W3015853029 cites W2113439382 @default.
• W3015853029 cites W2114818342 @default.
• W3015853029 cites W2134089325 @default.
• W3015853029 cites W2138390185 @default.
• W3015853029 cites W2142055951 @default.
• W3015853029 cites W2150790075 @default.
• W3015853029 cites W2152250071 @default.
• W3015853029 cites W2203807603 @default.
• W3015853029 cites W2320132985 @default.
• W3015853029 cites W2388525899 @default.
• W3015853029 cites W2417481612 @default.
• W3015853029 cites W2517492442 @default.
• W3015853029 cites W2518292292 @default.
• W3015853029 cites W2569673597 @default.
• W3015853029 cites W2585859826 @default.
• W3015853029 cites W2736273628 @default.
• W3015853029 cites W2752661497 @default.
• W3015853029 cites W2755662361 @default.
• W3015853029 cites W2755804840 @default.
• W3015853029 cites W2766424687 @default.
• W3015853029 cites W2773291920 @default.
• W3015853029 cites W2795624999 @default.
• W3015853029 cites W2800834635 @default.
• W3015853029 cites W2807507381 @default.
• W3015853029 cites W2887089079 @default.
• W3015853029 cites W2891617613 @default.
• W3015853029 cites W2938821856 @default.
• W3015853029 cites W2939572941 @default.
• W3015853029 cites W2964165737 @default.
• W3015853029 doi "https://doi.org/10.1074/jbc.ra120.012646" @default.
• W3015853029 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7247301" @default.
• W3015853029 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33516347" @default.
• W3015853029 hasPublicationYear "2020" @default.
• W3015853029 type Work @default.
• W3015853029 sameAs 3015853029 @default.
• W3015853029 citedByCount "19" @default.
• W3015853029 countsByYear W30158530292020 @default.
• W3015853029 countsByYear W30158530292021 @default.
• W3015853029 countsByYear W30158530292022 @default.
• W3015853029 countsByYear W30158530292023 @default.
• W3015853029 crossrefType "journal-article" @default.
• W3015853029 hasAuthorship W3015853029A5012463920 @default.
• W3015853029 hasAuthorship W3015853029A5023306273 @default.
• W3015853029 hasAuthorship W3015853029A5024961418 @default.
• W3015853029 hasAuthorship W3015853029A5027004504 @default.
• W3015853029 hasAuthorship W3015853029A5032273314 @default.
• W3015853029 hasAuthorship W3015853029A5036379634 @default.
• W3015853029 hasAuthorship W3015853029A5061317559 @default.
• W3015853029 hasAuthorship W3015853029A5068725892 @default.
• W3015853029 hasAuthorship W3015853029A5090802023 @default.
• W3015853029 hasBestOaLocation W30158530291 @default.
• W3015853029 hasConcept C104317684 @default.
• W3015853029 hasConcept C203014093 @default.

• next