FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3016134137> ?p ?o ?g. }

• W3016134137 abstract "Abstract Bacteriophage technologies have immense potential as antibiotic therapies and in genetic engineering. Understanding the mechanisms that bacteriophages implement to infect their hosts will allow researchers to manipulate these systems and adapt them to specific bacterial targets. Here, we isolated a bacteriophage capable of infecting the marine alphaproteobacterium Phaeobacter inhibens and dissected its mechanism of infection. Phaeobacter phage MD18, a novel species of bacteriophage isolated in Woods Hole, MA, exhibits potent lytic ability against P. inhibens and appears to be of the Siphoviridae morphotype. Consistent with this finding, the sequence of the MD18 revealed significant similarity to another siphophage, the recently discovered Roseobacter phage DSS3P8. We incubated MD18 with a library of barcoded P. inhibens transposon insertion mutants and identified 22 genes that appear to be required for phage predation of this host. Network analysis of these genes using genomic position, GO term enrichment, and protein associations reveals that these genes are enriched for roles in assembly of a type IV pilus (T4P) and regulators of cellular morphology. Our results suggest that T4P serve as receptors for a novel marine virus that targets P. inhibens . Importance Bacteriophages are useful non-antibiotic therapeutics for bacterial infections as well as threats to industries utilizing bacterial agents. This study identifies Phaeobacter phage MD18, the first documented phage of Phaeobacter inhibens , a bacterium with promising use as a probiotic for aquatic farming industries. Genomic analysis suggests that the P haeobacter phage MD18 has evolved to enhance its replication in P. inhibens by adopting favorable tRNA genes as well as through genomic sequence adaptation to resemble host codon usage. Lastly, a high-throughput analysis of P. inhibens transposon insertion mutants identifies genes that modulate host susceptibility to phage MD18 and implicates the type IV pilus as the likely receptor recognized for adsorption. This study marks the first characterization of the relationship between P. inhibens and an environmentally sampled phage, which informs our understanding of natural threats to the bacterium and may promote the development of novel phage technologies for genetic manipulation of this host." @default.
• W3016134137 created "2020-04-17" @default.
• W3016134137 creator A5000063587 @default.
• W3016134137 creator A5036077732 @default.
• W3016134137 creator A5055058443 @default.
• W3016134137 creator A5060381978 @default.
• W3016134137 creator A5073640390 @default.
• W3016134137 date "2020-04-13" @default.
• W3016134137 modified "2023-10-17" @default.
• W3016134137 title "Discovering the Molecular Determinants of Phaeobacter inhibens susceptibility to Phaeobacter phage MD18" @default.
• W3016134137 cites W1833156023 @default.
• W3016134137 cites W1898780412 @default.
• W3016134137 cites W1973465971 @default.
• W3016134137 cites W1975778959 @default.
• W3016134137 cites W1979471280 @default.
• W3016134137 cites W1985563130 @default.
• W3016134137 cites W1988967448 @default.
• W3016134137 cites W1995193749 @default.
• W3016134137 cites W2004548026 @default.
• W3016134137 cites W2015290513 @default.
• W3016134137 cites W2016838133 @default.
• W3016134137 cites W2018949401 @default.
• W3016134137 cites W2027826836 @default.
• W3016134137 cites W2043600088 @default.
• W3016134137 cites W2076936691 @default.
• W3016134137 cites W2078908111 @default.
• W3016134137 cites W2091030812 @default.
• W3016134137 cites W2104878600 @default.
• W3016134137 cites W2106407107 @default.
• W3016134137 cites W2106673630 @default.
• W3016134137 cites W2111772170 @default.
• W3016134137 cites W2122420189 @default.
• W3016134137 cites W2124704072 @default.
• W3016134137 cites W2138608758 @default.
• W3016134137 cites W2142428670 @default.
• W3016134137 cites W2145866605 @default.
• W3016134137 cites W2165059275 @default.
• W3016134137 cites W2173692600 @default.
• W3016134137 cites W2306546467 @default.
• W3016134137 cites W2393535458 @default.
• W3016134137 cites W2401437979 @default.
• W3016134137 cites W2477514062 @default.
• W3016134137 cites W2504389924 @default.
• W3016134137 cites W2594630120 @default.
• W3016134137 cites W2741628575 @default.
• W3016134137 cites W2767774935 @default.
• W3016134137 cites W2804515387 @default.
• W3016134137 cites W2900569176 @default.
• W3016134137 cites W2941342985 @default.
• W3016134137 cites W2949853177 @default.
• W3016134137 cites W2950020631 @default.
• W3016134137 cites W2969940543 @default.
• W3016134137 doi "https://doi.org/10.1101/2020.04.13.038638" @default.
• W3016134137 hasPublicationYear "2020" @default.
• W3016134137 type Work @default.
• W3016134137 sameAs 3016134137 @default.
• W3016134137 citedByCount "1" @default.
• W3016134137 countsByYear W30161341372020 @default.
• W3016134137 crossrefType "posted-content" @default.
• W3016134137 hasAuthorship W3016134137A5000063587 @default.
• W3016134137 hasAuthorship W3016134137A5036077732 @default.
• W3016134137 hasAuthorship W3016134137A5055058443 @default.
• W3016134137 hasAuthorship W3016134137A5060381978 @default.
• W3016134137 hasAuthorship W3016134137A5073640390 @default.
• W3016134137 hasBestOaLocation W30161341371 @default.
• W3016134137 hasConcept C104317684 @default.
• W3016134137 hasConcept C174832134 @default.
• W3016134137 hasConcept C182615771 @default.
• W3016134137 hasConcept C193252679 @default.
• W3016134137 hasConcept C2522874641 @default.
• W3016134137 hasConcept C2776441376 @default.
• W3016134137 hasConcept C2777693772 @default.
• W3016134137 hasConcept C2778303719 @default.
• W3016134137 hasConcept C44465124 @default.
• W3016134137 hasConcept C54355233 @default.
• W3016134137 hasConcept C547475151 @default.
• W3016134137 hasConcept C60987743 @default.
• W3016134137 hasConcept C70721500 @default.
• W3016134137 hasConcept C86803240 @default.
• W3016134137 hasConcept C89423630 @default.
• W3016134137 hasConceptScore W3016134137C104317684 @default.
• W3016134137 hasConceptScore W3016134137C174832134 @default.
• W3016134137 hasConceptScore W3016134137C182615771 @default.
• W3016134137 hasConceptScore W3016134137C193252679 @default.
• W3016134137 hasConceptScore W3016134137C2522874641 @default.
• W3016134137 hasConceptScore W3016134137C2776441376 @default.
• W3016134137 hasConceptScore W3016134137C2777693772 @default.
• W3016134137 hasConceptScore W3016134137C2778303719 @default.
• W3016134137 hasConceptScore W3016134137C44465124 @default.
• W3016134137 hasConceptScore W3016134137C54355233 @default.
• W3016134137 hasConceptScore W3016134137C547475151 @default.
• W3016134137 hasConceptScore W3016134137C60987743 @default.
• W3016134137 hasConceptScore W3016134137C70721500 @default.
• W3016134137 hasConceptScore W3016134137C86803240 @default.
• W3016134137 hasConceptScore W3016134137C89423630 @default.
• W3016134137 hasLocation W30161341371 @default.
• W3016134137 hasLocation W30161341372 @default.
• W3016134137 hasOpenAccess W3016134137 @default.
• W3016134137 hasPrimaryLocation W30161341371 @default.
• W3016134137 hasRelatedWork W17375570 @default.

• next