Matches in SemOpenAlex for { <https://semopenalex.org/work/W3016433696> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W3016433696 endingPage "47" @default.
- W3016433696 startingPage "41" @default.
- W3016433696 abstract "We present a novel method for concentration and purification of DNA from biological samples. The method is based on isotachophoretic separation of DNA strands in a separation bed made of a disposable nonwoven fabric strip. Application of oxalate as the leading ion prevented corrosion of the carbon anode and also the leading ion was continually removed from the system due to its decomposition into CO2 at the anode. The fractions were marked by three colored markers of electrophoretic mobility closely surrounding the mobility of DNA. The fraction collection was realized by a centrifugal drain of cut out strip segments. The method was evaluated using two purified salmon sperm DNA fragments of lengths 200 bp and 2000 bp. The results confirmed the high DNA concentrating effect of the method (34-fold increase of the original DNA concentration). The composition of running solutions and voltage program were optimized in order to finish the analysis within 30 min. The optimized method was used to extract, concentrate and purify DNA from a crude yeast cell lysate. The maximum DNA enrichment factor decreased to 12 due to the stretching of DNA zones caused by low-molecular contaminants present in the original lysate. The average recovery determined for yeast DNA was 71 ± 11% (n = 3). The connected elimination of the proteins from DNA zones resulted in the purification factor value of 582 for DNA vs proteins. This demonstrates that the presented method is capable to concentrate DNA from the bulk volume and to further purify it from crude cell lysates using a simple instrumentation and low-cost disposable separation bed." @default.
- W3016433696 created "2020-04-24" @default.
- W3016433696 creator A5006627605 @default.
- W3016433696 creator A5019097665 @default.
- W3016433696 creator A5045900796 @default.
- W3016433696 date "2020-06-01" @default.
- W3016433696 modified "2023-10-18" @default.
- W3016433696 title "DNA purification and concentration by isotachophoresis in nonwoven fabric strip" @default.
- W3016433696 cites W1504286848 @default.
- W3016433696 cites W1583260256 @default.
- W3016433696 cites W1769550220 @default.
- W3016433696 cites W1992078865 @default.
- W3016433696 cites W2047127607 @default.
- W3016433696 cites W2059587847 @default.
- W3016433696 cites W2068007161 @default.
- W3016433696 cites W2085296413 @default.
- W3016433696 cites W2089157765 @default.
- W3016433696 cites W2113558688 @default.
- W3016433696 cites W2133374580 @default.
- W3016433696 cites W2142468717 @default.
- W3016433696 cites W2605453253 @default.
- W3016433696 cites W2752713907 @default.
- W3016433696 cites W2755717863 @default.
- W3016433696 cites W2760996111 @default.
- W3016433696 cites W2791555814 @default.
- W3016433696 cites W2882996908 @default.
- W3016433696 cites W2891854413 @default.
- W3016433696 cites W2943981201 @default.
- W3016433696 doi "https://doi.org/10.1016/j.aca.2020.04.029" @default.
- W3016433696 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32408953" @default.
- W3016433696 hasPublicationYear "2020" @default.
- W3016433696 type Work @default.
- W3016433696 sameAs 3016433696 @default.
- W3016433696 citedByCount "3" @default.
- W3016433696 countsByYear W30164336962022 @default.
- W3016433696 countsByYear W30164336962023 @default.
- W3016433696 crossrefType "journal-article" @default.
- W3016433696 hasAuthorship W3016433696A5006627605 @default.
- W3016433696 hasAuthorship W3016433696A5019097665 @default.
- W3016433696 hasAuthorship W3016433696A5045900796 @default.
- W3016433696 hasConcept C147789679 @default.
- W3016433696 hasConcept C17525397 @default.
- W3016433696 hasConcept C178790620 @default.
- W3016433696 hasConcept C185592680 @default.
- W3016433696 hasConcept C186293402 @default.
- W3016433696 hasConcept C2776416690 @default.
- W3016433696 hasConcept C40250595 @default.
- W3016433696 hasConcept C43617362 @default.
- W3016433696 hasConcept C4725764 @default.
- W3016433696 hasConcept C519885992 @default.
- W3016433696 hasConcept C552990157 @default.
- W3016433696 hasConcept C55493867 @default.
- W3016433696 hasConcept C57409179 @default.
- W3016433696 hasConcept C68801617 @default.
- W3016433696 hasConcept C89395315 @default.
- W3016433696 hasConceptScore W3016433696C147789679 @default.
- W3016433696 hasConceptScore W3016433696C17525397 @default.
- W3016433696 hasConceptScore W3016433696C178790620 @default.
- W3016433696 hasConceptScore W3016433696C185592680 @default.
- W3016433696 hasConceptScore W3016433696C186293402 @default.
- W3016433696 hasConceptScore W3016433696C2776416690 @default.
- W3016433696 hasConceptScore W3016433696C40250595 @default.
- W3016433696 hasConceptScore W3016433696C43617362 @default.
- W3016433696 hasConceptScore W3016433696C4725764 @default.
- W3016433696 hasConceptScore W3016433696C519885992 @default.
- W3016433696 hasConceptScore W3016433696C552990157 @default.
- W3016433696 hasConceptScore W3016433696C55493867 @default.
- W3016433696 hasConceptScore W3016433696C57409179 @default.
- W3016433696 hasConceptScore W3016433696C68801617 @default.
- W3016433696 hasConceptScore W3016433696C89395315 @default.
- W3016433696 hasFunder F4320311333 @default.
- W3016433696 hasFunder F4320321006 @default.
- W3016433696 hasFunder F4320322482 @default.
- W3016433696 hasLocation W30164336961 @default.
- W3016433696 hasOpenAccess W3016433696 @default.
- W3016433696 hasPrimaryLocation W30164336961 @default.
- W3016433696 hasRelatedWork W2001081022 @default.
- W3016433696 hasRelatedWork W2042774977 @default.
- W3016433696 hasRelatedWork W2043221075 @default.
- W3016433696 hasRelatedWork W2052810045 @default.
- W3016433696 hasRelatedWork W2054535766 @default.
- W3016433696 hasRelatedWork W2064324638 @default.
- W3016433696 hasRelatedWork W2079879048 @default.
- W3016433696 hasRelatedWork W2086792757 @default.
- W3016433696 hasRelatedWork W2908557677 @default.
- W3016433696 hasRelatedWork W3016433696 @default.
- W3016433696 hasVolume "1117" @default.
- W3016433696 isParatext "false" @default.
- W3016433696 isRetracted "false" @default.
- W3016433696 magId "3016433696" @default.
- W3016433696 workType "article" @default.