FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3016502156> ?p ?o ?g. }

• W3016502156 abstract "Abstract Campylobacter is the leading cause of bacterial foodborne gastroenteritis in many countries. Source attribution studies unequivocally identify the handling or consumption of contaminated poultry meat as the primary risk factor. One potential strategy to control Campylobacter is to select poultry with increased resistance to colonisation. We conducted genomic and transcriptomic analyses of commercial pedigree broilers exposed to Campylobacter to examine persistent colonisation of the caecum as a quantitative trait. 3,000 broilers were genotyped using a 50K single nucleotide polymorphism (SNP) array and imputed to 600K SNPs. Genotypes were analysed for associations with the number of viable Campylobacter in the caeca. Heritability of the trait was modest but significantly greater than zero (h 2 =0.11 ± 0.03). Genome-wide association analyses confirmed quantitative trait loci (QTL) on chromosomes 14 and 16 previously identified using the progeny of crosses of inbred lines differing in resistance, and detected two additional genome-wide significant QTLs on chromosomes 19 and 26. RNA-Seq analysis of the transcriptome of caecal tonsils from birds at the low and high extremes of C. jejuni colonisation phenotype identified differentially transcribed genes, mainly located within the QTL on chromosome 16 and proximal to the major histocompatibility complex (MHC) locus. We also identified strong cis -QTLs located within the MHC suggesting the presence of cis -acting variation in both MHC class I, class II and BG genes. Multiple other cis- acting variants were identified in association with key immune genes ( COPS3, CCL4, CR1L, C4BP, PLGR ) in the other QTLs. Pathway and network analysis implicated cooperative functional pathways and networks in colonisation, including those related to antigen presentation, innate and adaptive immune responses, calcium, and renin-angiotensin signalling. While co-selection for enhanced resistance and other breeding goal traits is feasible, the frequency of resistance-associated alleles was high in the population studied and non-genetic factors significantly influence Campylobacter colonisation in poultry. Author summary Campylobacter infection is estimated to cause 95 million illnesses in people worldwide each year. Human infections mostly involve gastroenteritis, but can have severe complications. The handling or consumption of contaminated poultry meat is a key risk factor for human campylobacteriosis. The bacteria reach high numbers in the intestines of chickens reared for meat (broilers) and are frequently found on carcasses after slaughter. Effective vaccines against Campylobacter are not yet available, and treatments to reduce carcass contamination (e.g. chlorination) are not acceptable in some markets. One alternative is to breed for chickens with improved resistance to Campylobacter colonisation. To test the feasibility of this option in commercial birds, we analysed the genetic make-up of 3,000 pedigree broilers and determined the number of Campylobacter in their gut. There were associations between specific regions of the chicken genome and resistance to Campylobacter . Within some of these regions, expression of certain genes differed between birds at the low and high extremes of Campylobacter colonisation, providing a potential explanation for genetic variation in resistance. Selection of poultry with increased resistance to Campylobacter colonisation may be a complementary strategy to improved biosecurity, management, handling and processing procedures to reduce the burden of Campylobacter on human health." @default.
• W3016502156 created "2020-04-24" @default.
• W3016502156 creator A5007891123 @default.
• W3016502156 creator A5010949104 @default.
• W3016502156 creator A5020529513 @default.
• W3016502156 creator A5022355556 @default.
• W3016502156 creator A5024372488 @default.
• W3016502156 creator A5031551214 @default.
• W3016502156 creator A5035936772 @default.
• W3016502156 creator A5037712270 @default.
• W3016502156 creator A5040689434 @default.
• W3016502156 creator A5047799975 @default.
• W3016502156 creator A5054927983 @default.
• W3016502156 creator A5063016994 @default.
• W3016502156 creator A5063543115 @default.
• W3016502156 creator A5071973746 @default.
• W3016502156 creator A5079832331 @default.
• W3016502156 creator A5080585460 @default.
• W3016502156 creator A5086500057 @default.
• W3016502156 date "2020-04-14" @default.
• W3016502156 modified "2023-10-11" @default.
• W3016502156 title "Genetic control of Campylobacter colonisation in broiler chickens: genomic and transcriptomic characterisation" @default.
• W3016502156 cites W1491365253 @default.
• W3016502156 cites W1849301422 @default.
• W3016502156 cites W1902302867 @default.
• W3016502156 cites W1952660301 @default.
• W3016502156 cites W1965288671 @default.
• W3016502156 cites W1966728354 @default.
• W3016502156 cites W1992991186 @default.
• W3016502156 cites W1993830627 @default.
• W3016502156 cites W1996221661 @default.
• W3016502156 cites W2015404596 @default.
• W3016502156 cites W2018644256 @default.
• W3016502156 cites W2019983262 @default.
• W3016502156 cites W2031441922 @default.
• W3016502156 cites W2032350671 @default.
• W3016502156 cites W2041706071 @default.
• W3016502156 cites W2049363596 @default.
• W3016502156 cites W2060320549 @default.
• W3016502156 cites W2077120454 @default.
• W3016502156 cites W2089158517 @default.
• W3016502156 cites W2091870152 @default.
• W3016502156 cites W2094367664 @default.
• W3016502156 cites W2100262918 @default.
• W3016502156 cites W2100697281 @default.
• W3016502156 cites W2102245641 @default.
• W3016502156 cites W2103814293 @default.
• W3016502156 cites W2106187422 @default.
• W3016502156 cites W2107338948 @default.
• W3016502156 cites W2107998771 @default.
• W3016502156 cites W2108234281 @default.
• W3016502156 cites W2113908072 @default.
• W3016502156 cites W2122683221 @default.
• W3016502156 cites W2123806956 @default.
• W3016502156 cites W2131271579 @default.
• W3016502156 cites W2131935632 @default.
• W3016502156 cites W2133869967 @default.
• W3016502156 cites W2134772950 @default.
• W3016502156 cites W2135597751 @default.
• W3016502156 cites W2135716845 @default.
• W3016502156 cites W2136415990 @default.
• W3016502156 cites W2137864720 @default.
• W3016502156 cites W2138130000 @default.
• W3016502156 cites W2146018868 @default.
• W3016502156 cites W2146282992 @default.
• W3016502156 cites W2148114069 @default.
• W3016502156 cites W2149594592 @default.
• W3016502156 cites W2150917924 @default.
• W3016502156 cites W2152005348 @default.
• W3016502156 cites W2154921479 @default.
• W3016502156 cites W2155198998 @default.
• W3016502156 cites W2158284941 @default.
• W3016502156 cites W2161245845 @default.
• W3016502156 cites W2161633633 @default.
• W3016502156 cites W2162143298 @default.
• W3016502156 cites W2168122182 @default.
• W3016502156 cites W2169456326 @default.
• W3016502156 cites W2185442575 @default.
• W3016502156 cites W2186140012 @default.
• W3016502156 cites W2191779450 @default.
• W3016502156 cites W2199798003 @default.
• W3016502156 cites W2226959884 @default.
• W3016502156 cites W2271017908 @default.
• W3016502156 cites W2298492059 @default.
• W3016502156 cites W2323326409 @default.
• W3016502156 cites W2342381719 @default.
• W3016502156 cites W2346396209 @default.
• W3016502156 cites W2516882219 @default.
• W3016502156 cites W2539538064 @default.
• W3016502156 cites W2541369470 @default.
• W3016502156 cites W2552622217 @default.
• W3016502156 cites W2586599127 @default.
• W3016502156 cites W2606590632 @default.
• W3016502156 cites W2620721366 @default.
• W3016502156 cites W2747961425 @default.
• W3016502156 cites W2805916328 @default.
• W3016502156 cites W2810015247 @default.
• W3016502156 cites W2810793067 @default.
• W3016502156 cites W2888494779 @default.
• W3016502156 cites W2891778779 @default.

• next