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• W3016544360 abstract "Anisakis pegreffii is known as one of the causes of a fish-borne zoonosis, anisakidosis. Despite its significant public health and food hygiene impacts, little is known of the pathogenesis, genetic background of this parasite, at least partly due to the lack of genome and transcriptome information. In this study, RNA-seq and de novo assembly were conducted to obtain transcriptome profiles of the A. pegreffii third and fourth larvae. The third stage larvae (APL3) were collected from chub mackerel and the fourth stage larvae (APL4) were obtained by in vitro culture. In total, 47,243 and 43,660 unigenes were expressed in APL3 and APL4 transcriptomes. Of them, 18,753 were known and 28,490 were novel for APL3, while 18,996 were known and 24,664 were novel for APL4. The most abundantly expressed genes in APL3 were mitochondrial enzymes (COI, COII, COIII) and polyubiquitins (UBB, UBIQP_XENLA). Collagen-related genes (col-145, col-34, col-138, Bm1_54705, col-40) were the most abundantly expressed in APL4. Mitochondrial enzyme genes (COIII, COI) were also highly expressed in APL4. Among the transcripts, 614 were up-regulated in APL3, while 1,309 were up-regulated in APL4. Several protease and protein biosynthesis-related genes were highly expressed in APL3, all of which are thought to be crucial for invading host tissues. Collagen synthesis-related genes were highly expressed in APL4, reflecting active biosynthesis of collagens occurs during moulting process of APL4. Of these differentially expressed genes, several genes (SI, nas-13, EF-TSMT, SFXN2, dhs-27) were validated to highly transcribed in APL3, while other genes (col-40, F09E10.7, pept-1, col-34, VIT) in APL4. The biological roles of these genes in vivo will be deciphered when the reference genome sequences are available, together with in vitro experiments.Anisakis pegreffii is known as one of the causes of a fish-borne zoonosis, anisakidosis. Despite its significant public health and food hygiene impacts, little is known of the pathogenesis, genetic background of this parasite, at least partly due to the lack of genome and transcriptome information. In this study, RNA-seq and de novo assembly were conducted to obtain transcriptome profiles of the A. pegreffii third and fourth larvae. The third stage larvae (APL3) were collected from chub mackerel and the fourth stage larvae (APL4) were obtained by in vitro culture. In total, 47,243 and 43,660 unigenes were expressed in APL3 and APL4 transcriptomes. Of them, 18,753 were known and 28,490 were novel for APL3, while 18,996 were known and 24,664 were novel for APL4. The most abundantly expressed genes in APL3 were mitochondrial enzymes (COI, COII, COIII) and polyubiquitins (UBB, UBIQP_XENLA). Collagen-related genes (col-145, col-34, col-138, Bm1_54705, col-40) were the most abundantly expressed in APL4. Mitochondrial enzyme genes (COIII, COI) were also highly expressed in APL4. Among the transcripts, 614 were up-regulated in APL3, while 1,309 were up-regulated in APL4. Several protease and protein biosynthesis-related genes were highly expressed in APL3, all of which are thought to be crucial for invading host tissues. Collagen synthesis-related genes were highly expressed in APL4, reflecting active biosynthesis of collagens occurs during moulting process of APL4. Of these differentially expressed genes, several genes (SI, nas-13, EF-TSMT, SFXN2, dhs-27) were validated to highly transcribed in APL3, while other genes (col-40, F09E10.7, pept-1, col-34, VIT) in APL4. The biological roles of these genes in vivo will be deciphered when the reference genome sequences are available, together with in vitro experiments." @default.
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• W3016544360 date "2020-01-01" @default.
• W3016544360 modified "2023-09-27" @default.
• W3016544360 title "De novo transcriptome sequencing and analysis of <i>Anisakis pegreffii</i> (Nematoda: Anisakidae) third-stage and fourth stage larvae" @default.
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• W3016544360 cites W1965162775 @default.
• W3016544360 cites W1975410482 @default.
• W3016544360 cites W1976472052 @default.
• W3016544360 cites W1977383152 @default.
• W3016544360 cites W1982212928 @default.
• W3016544360 cites W1985782564 @default.
• W3016544360 cites W1985866273 @default.
• W3016544360 cites W1999520447 @default.
• W3016544360 cites W1999574084 @default.
• W3016544360 cites W2011657487 @default.
• W3016544360 cites W2014954966 @default.
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• W3016544360 cites W2027413277 @default.
• W3016544360 cites W2028890113 @default.
• W3016544360 cites W2053495553 @default.
• W3016544360 cites W2073421597 @default.
• W3016544360 cites W2076362746 @default.
• W3016544360 cites W2078346621 @default.
• W3016544360 cites W2089157584 @default.
• W3016544360 cites W2095478773 @default.
• W3016544360 cites W2096939566 @default.
• W3016544360 cites W2101439782 @default.
• W3016544360 cites W2101972919 @default.
• W3016544360 cites W2103441770 @default.
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• W3016544360 doi "https://doi.org/10.21307/jofnem-2020-041" @default.
• W3016544360 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7266050" @default.
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