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- W3016738511 abstract "Petroselinic acid (18:1Δ6), a monounsaturated cis Δ-6 fatty acid, has many prospective applications in functional foods and for the nutraceutical and pharmaceutical industries. Up to 80% of petroselinic acid has been found in the oil from fruits of coriander (Coriandrum sativum L.), which make it an ideal source for investigating the biosynthesis of petroselinic acid. A coriander acyl–acyl carrier protein desaturase was identified to be involved in its biosynthesis more than two decades ago, but since then little further progress in this area has been reported. In this study, the fatty acid profiles of coriander fruits at six developmental stages were analyzed. Fruit samples from three developmental stages with rapid accumulation of petroselinic acid were used for RNA sequencing using the Illumina Hiseq4000 platform. The transcriptome analysis presented 93 323 nonredundant unigenes and 8545 differentially expressed genes. Functional annotation and combined gene expression data revealed candidate genes potentially involved in petroselinic acid biosynthesis and its incorporation into triacylglycerols. Tissue-specific examination of q-PCR validation further suggested that ACPD1/3, KAS I-1, FATB-1/3, and DGAT2 may be highly involved. Bioinformatic analysis of CsFATB and CsDGAT2 identified their putative key amino acids or functional motifs. These results provide a molecular foundation for petroselinic acid biosynthesis in coriander fruit and facilitate its genetic engineering in other hosts." @default.
- W3016738511 created "2020-04-24" @default.
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- W3016738511 date "2020-04-22" @default.
- W3016738511 modified "2023-10-16" @default.
- W3016738511 title "Transcriptome Analysis Reveals Candidate Genes for Petroselinic Acid Biosynthesis in Fruits of <i>Coriandrum sativum</i> L." @default.
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- W3016738511 doi "https://doi.org/10.1021/acs.jafc.0c01487" @default.
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