Matches in SemOpenAlex for { <https://semopenalex.org/work/W3016817118> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W3016817118 endingPage "1" @default.
- W3016817118 startingPage "1" @default.
- W3016817118 abstract "Dipteran Na+-dependent cation-chloride cotransporters (CCCs) group into two clades. Drosophila melanogaster Ncc69 is a bona fide Na+-K+-Cl− cotransporter and has a single ortholog in Aedes aegypti (aeNKCC1). Drosophila Ncc83 has not been physiologically characterized and has two orthologs in Ae. aegypti (aeCCC2 and aeCCC3). Previous work showed that oocytes expressing aeCCC2 have higher Na+ conductances than controls. Moreover, in flux assays using Li+ as a Na+ tracer, Li+ transport by aeCCC2 was not dependent upon extracellular Cl− and not inhibited by the loop diuretics furosemide or bumetanide. These are surprising results, since previously characterized CCCs are electroneutral, Cl−-dependent, and sensitive to the loop diuretics. We have further characterized aeCCC2 and performed an initial analysis of Ncc83, using two-electrode voltage clamp. Following a hypotonic pre-incubation, changes in membrane potential upon Na+ replacement were 20 ± 3 mV in Ncc83 and 26 ± 4 mV in aeCCC2, approximately two-fold greater than before swelling. Na+-dependent currents in oocytes expressing aeCCC2 depended on extracellular Na+ with saturable Michaelis-Menton kinetics. The apparent affinity for Na+ was 4.6 ± 0.5 mM (mean, SEM, n=5). To characterize the function of Ncc83 in Drosophila Malpighian tubules (MT), we developed procedures to assess the cation concentrations of secreted fluid in Ramsay secretion assays using cation-exchange chromatography. The physiological cations were readily detectable in 10,000–50,000 fold dilutions of secreted fluid, and Li+ and Rb+ were also detectable when added to the bathing saline as tracers for Na+ and K+. In w118-control flies, fluid secreted by MT measured using these procedures had a [K+] of 104.1 ± 8.8 mM (mean, SEM, n=7) and [Na+] of 61.2 ± 7.8 mM. In preliminary experiments, [K+] was 112.7 ± 1.3 mM (n=4) and [Na+] was 48.2 ± 4.1 mM in fluid secreted by MTs of flies with MT-specific knockdown of Ncc83 using the GAL4-UAS RNAi system. Further work is needed to determine the physiological roles of Ncc83 and aeCCC2. Funding: American Physiological Society Research Career Enhancement Award, NIH F33 GM131599, and Kenyon College. Support or Funding Information Funding: American Physiological Society Research Career Enhancement Award, NIH F33 GM131599, and Kenyon College." @default.
- W3016817118 created "2020-04-24" @default.
- W3016817118 creator A5031198630 @default.
- W3016817118 creator A5041914018 @default.
- W3016817118 creator A5052621942 @default.
- W3016817118 creator A5083441262 @default.
- W3016817118 date "2020-04-01" @default.
- W3016817118 modified "2023-10-18" @default.
- W3016817118 title "Functional analysis of mosquito and Drosophila Na + ‐dependent cation‐chloride cotransporters" @default.
- W3016817118 doi "https://doi.org/10.1096/fasebj.2020.34.s1.02934" @default.
- W3016817118 hasPublicationYear "2020" @default.
- W3016817118 type Work @default.
- W3016817118 sameAs 3016817118 @default.
- W3016817118 citedByCount "0" @default.
- W3016817118 crossrefType "journal-article" @default.
- W3016817118 hasAuthorship W3016817118A5031198630 @default.
- W3016817118 hasAuthorship W3016817118A5041914018 @default.
- W3016817118 hasAuthorship W3016817118A5052621942 @default.
- W3016817118 hasAuthorship W3016817118A5083441262 @default.
- W3016817118 hasBestOaLocation W30168171181 @default.
- W3016817118 hasConcept C100837961 @default.
- W3016817118 hasConcept C104317684 @default.
- W3016817118 hasConcept C121332964 @default.
- W3016817118 hasConcept C12554922 @default.
- W3016817118 hasConcept C148898269 @default.
- W3016817118 hasConcept C178790620 @default.
- W3016817118 hasConcept C181911157 @default.
- W3016817118 hasConcept C185592680 @default.
- W3016817118 hasConcept C188053792 @default.
- W3016817118 hasConcept C2778212192 @default.
- W3016817118 hasConcept C2778695967 @default.
- W3016817118 hasConcept C2780104201 @default.
- W3016817118 hasConcept C28406088 @default.
- W3016817118 hasConcept C41625074 @default.
- W3016817118 hasConcept C537181965 @default.
- W3016817118 hasConcept C55493867 @default.
- W3016817118 hasConcept C57181701 @default.
- W3016817118 hasConcept C62520636 @default.
- W3016817118 hasConcept C74884574 @default.
- W3016817118 hasConcept C86803240 @default.
- W3016817118 hasConcept C99975950 @default.
- W3016817118 hasConceptScore W3016817118C100837961 @default.
- W3016817118 hasConceptScore W3016817118C104317684 @default.
- W3016817118 hasConceptScore W3016817118C121332964 @default.
- W3016817118 hasConceptScore W3016817118C12554922 @default.
- W3016817118 hasConceptScore W3016817118C148898269 @default.
- W3016817118 hasConceptScore W3016817118C178790620 @default.
- W3016817118 hasConceptScore W3016817118C181911157 @default.
- W3016817118 hasConceptScore W3016817118C185592680 @default.
- W3016817118 hasConceptScore W3016817118C188053792 @default.
- W3016817118 hasConceptScore W3016817118C2778212192 @default.
- W3016817118 hasConceptScore W3016817118C2778695967 @default.
- W3016817118 hasConceptScore W3016817118C2780104201 @default.
- W3016817118 hasConceptScore W3016817118C28406088 @default.
- W3016817118 hasConceptScore W3016817118C41625074 @default.
- W3016817118 hasConceptScore W3016817118C537181965 @default.
- W3016817118 hasConceptScore W3016817118C55493867 @default.
- W3016817118 hasConceptScore W3016817118C57181701 @default.
- W3016817118 hasConceptScore W3016817118C62520636 @default.
- W3016817118 hasConceptScore W3016817118C74884574 @default.
- W3016817118 hasConceptScore W3016817118C86803240 @default.
- W3016817118 hasConceptScore W3016817118C99975950 @default.
- W3016817118 hasIssue "S1" @default.
- W3016817118 hasLocation W30168171181 @default.
- W3016817118 hasOpenAccess W3016817118 @default.
- W3016817118 hasPrimaryLocation W30168171181 @default.
- W3016817118 hasRelatedWork W1556717980 @default.
- W3016817118 hasRelatedWork W2060480841 @default.
- W3016817118 hasRelatedWork W2063342945 @default.
- W3016817118 hasRelatedWork W2077409172 @default.
- W3016817118 hasRelatedWork W2078455512 @default.
- W3016817118 hasRelatedWork W2096456941 @default.
- W3016817118 hasRelatedWork W2113568843 @default.
- W3016817118 hasRelatedWork W2123002852 @default.
- W3016817118 hasRelatedWork W2318921039 @default.
- W3016817118 hasRelatedWork W95909497 @default.
- W3016817118 hasVolume "34" @default.
- W3016817118 isParatext "false" @default.
- W3016817118 isRetracted "false" @default.
- W3016817118 magId "3016817118" @default.
- W3016817118 workType "article" @default.