Matches in SemOpenAlex for { <https://semopenalex.org/work/W3016860157> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W3016860157 endingPage "1" @default.
- W3016860157 startingPage "1" @default.
- W3016860157 abstract "The mechanism of action of mitochondrial targeted anti-cancer drugs relies on their ability to disrupt the energy producing systems of cancer cell mitochondria. Momordica charantia (MC), an acclaimed anticancer plant in folk medicine, is here investigated for the probable mechanism by which it kills cancer cells. The ethyl acetate (D3), n-hexane (D4) and dichloromethane (D5) fractions were obtained from the Crude Water-Soluble extract (CWSE) of MC via solvent partitioning and then administered on MDA-MB 436 and A549 cells at 100 and 125 μg/mL for 24 h. Cytofluorometric techniques, using TMRM and H2DCF-DA as fluorescent probes were employed to assess the effect of MC on mitochondrial membrane potential (MMP) and reactive oxygen species (ROS) generation respectively, while ATP levels were measured using the luciferin-luciferase reaction. Mean respiring cell numbers obtained from Dot graphs and Histograms were further analyzed, yielding significant (P<0.05) MMP depressions only at 125μg/mL in both MDA-MB 436 (43.08±2.33) and A549 (24.84±2.51) cells treated with D3 in comparison with untreated cells (175.8±6.13 and 131.7±11.21 respectively). In the same vein, only MDA-MB 436 cells treated with D3 and D5 at 100 and 125 μg/mL significantly elevated ROS levels in comparison with untreated (100.0±2.16) cells; the highest generation being 332.1±3.20, seen in D3-treated cells at 125 μg/mL. Furthermore, all fractions (D3-D5) tested on A549 cells at 100μg/mL and 125μg/mL generated significantly higher levels of ROS compared with the untreated (42±4.50) group. The highest observed levels of generated ROS were seen at 125μg/mL in the D5-treated cells (536±4.00). For intracellular ATP generation, significant (P<0.05) depletion was observed in both MDA-MB 436 cells [only at 125μg/mL of D3 (80.63±4.70) compared with the control group (106.7±5.69)] and A549 cells [D3 at 125 μg/mL (43.68±0.13) < D5 at 125 μg/mL (52.56±4.16) < D3 at 100 μg/mL (59.84±5.39) compared with the control group (98.57±0.66)]. On the whole, this study suggests that the tested fractions of MC induced a mitochondrial-dependent cell death in selected cancer cell lines. Support or Funding Information Organization for Women in Science for the Developing World (OWSD) and Swedish International Development Cooperation Agency (SIDA)" @default.
- W3016860157 created "2020-04-24" @default.
- W3016860157 creator A5001202059 @default.
- W3016860157 creator A5023277894 @default.
- W3016860157 creator A5049881179 @default.
- W3016860157 creator A5056294598 @default.
- W3016860157 creator A5057465266 @default.
- W3016860157 creator A5088553252 @default.
- W3016860157 date "2020-04-01" @default.
- W3016860157 modified "2023-09-26" @default.
- W3016860157 title "Treatment with Momordica charantia Fractions Disrupts Energy Metabolism and Elevated Reactive Oxygen Species’ Generation in Human Lung and Breast Cancer Cell Lines" @default.
- W3016860157 doi "https://doi.org/10.1096/fasebj.2020.34.s1.00276" @default.
- W3016860157 hasPublicationYear "2020" @default.
- W3016860157 type Work @default.
- W3016860157 sameAs 3016860157 @default.
- W3016860157 citedByCount "0" @default.
- W3016860157 crossrefType "journal-article" @default.
- W3016860157 hasAuthorship W3016860157A5001202059 @default.
- W3016860157 hasAuthorship W3016860157A5023277894 @default.
- W3016860157 hasAuthorship W3016860157A5049881179 @default.
- W3016860157 hasAuthorship W3016860157A5056294598 @default.
- W3016860157 hasAuthorship W3016860157A5057465266 @default.
- W3016860157 hasAuthorship W3016860157A5088553252 @default.
- W3016860157 hasBestOaLocation W30168601571 @default.
- W3016860157 hasConcept C121608353 @default.
- W3016860157 hasConcept C126322002 @default.
- W3016860157 hasConcept C1491633281 @default.
- W3016860157 hasConcept C153911025 @default.
- W3016860157 hasConcept C185592680 @default.
- W3016860157 hasConcept C190283241 @default.
- W3016860157 hasConcept C2777366897 @default.
- W3016860157 hasConcept C2778175917 @default.
- W3016860157 hasConcept C2778686829 @default.
- W3016860157 hasConcept C28859421 @default.
- W3016860157 hasConcept C48349386 @default.
- W3016860157 hasConcept C54355233 @default.
- W3016860157 hasConcept C55493867 @default.
- W3016860157 hasConcept C556039675 @default.
- W3016860157 hasConcept C71924100 @default.
- W3016860157 hasConcept C81729549 @default.
- W3016860157 hasConcept C81885089 @default.
- W3016860157 hasConcept C86803240 @default.
- W3016860157 hasConcept C96232424 @default.
- W3016860157 hasConcept C98274493 @default.
- W3016860157 hasConceptScore W3016860157C121608353 @default.
- W3016860157 hasConceptScore W3016860157C126322002 @default.
- W3016860157 hasConceptScore W3016860157C1491633281 @default.
- W3016860157 hasConceptScore W3016860157C153911025 @default.
- W3016860157 hasConceptScore W3016860157C185592680 @default.
- W3016860157 hasConceptScore W3016860157C190283241 @default.
- W3016860157 hasConceptScore W3016860157C2777366897 @default.
- W3016860157 hasConceptScore W3016860157C2778175917 @default.
- W3016860157 hasConceptScore W3016860157C2778686829 @default.
- W3016860157 hasConceptScore W3016860157C28859421 @default.
- W3016860157 hasConceptScore W3016860157C48349386 @default.
- W3016860157 hasConceptScore W3016860157C54355233 @default.
- W3016860157 hasConceptScore W3016860157C55493867 @default.
- W3016860157 hasConceptScore W3016860157C556039675 @default.
- W3016860157 hasConceptScore W3016860157C71924100 @default.
- W3016860157 hasConceptScore W3016860157C81729549 @default.
- W3016860157 hasConceptScore W3016860157C81885089 @default.
- W3016860157 hasConceptScore W3016860157C86803240 @default.
- W3016860157 hasConceptScore W3016860157C96232424 @default.
- W3016860157 hasConceptScore W3016860157C98274493 @default.
- W3016860157 hasIssue "S1" @default.
- W3016860157 hasLocation W30168601571 @default.
- W3016860157 hasOpenAccess W3016860157 @default.
- W3016860157 hasPrimaryLocation W30168601571 @default.
- W3016860157 hasRelatedWork W1984329481 @default.
- W3016860157 hasRelatedWork W2022267427 @default.
- W3016860157 hasRelatedWork W2344572926 @default.
- W3016860157 hasRelatedWork W2368940306 @default.
- W3016860157 hasRelatedWork W2384557518 @default.
- W3016860157 hasRelatedWork W2607808765 @default.
- W3016860157 hasRelatedWork W2751542945 @default.
- W3016860157 hasRelatedWork W2765087004 @default.
- W3016860157 hasRelatedWork W2791984512 @default.
- W3016860157 hasRelatedWork W4242986706 @default.
- W3016860157 hasVolume "34" @default.
- W3016860157 isParatext "false" @default.
- W3016860157 isRetracted "false" @default.
- W3016860157 magId "3016860157" @default.
- W3016860157 workType "article" @default.