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- W3016989069 abstract "Ran is an essential GTPase protein involved in bidirectional transportation between nucleus and cytoplasm. We engineered temperature sensitive (TS) mutant alleles of Ran accessory proteins in S. cerevisiae to study the genetic interactions. For essential proteins like Ran and its accessory proteins, TS mutants give insight into gene function without knocking out genes. By creating these TS mutant strains, we will be able to study the genetic interactions between Ran point mutants and TS mutant Ran accessory proteins to closely interrogate the biophysics and mechanism of the Ran GTPase cycle and it’s role nucleocytoplamsic transport. Support or Funding Information The 2019 NIH Summer Undergraduate Research Program at UMass Medical School. NIH Grant # R25 HL092610-11" @default.
- W3016989069 created "2020-04-24" @default.
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- W3016989069 date "2020-04-01" @default.
- W3016989069 modified "2023-10-18" @default.
- W3016989069 title "Engineering Temperature Sensitive Alleles in Saccharomyces cerevisiae to Study the Genetic Interactions Between Ran point mutants and its Accessory Proteins" @default.
- W3016989069 doi "https://doi.org/10.1096/fasebj.2020.34.s1.02170" @default.
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