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- W3017360034 abstract "High-pressure fluorometry using a polarity-sensitive membrane probe, Prodan, is a powerful technique that can investigate microscopic environments in phosphatidylcholine (PC) bilayers. The principle of Prodan fluorescencence spectroscopy and the procedure of bilayer imaging, that is, a construction of three-dimensional image plot based on the second derivative of the Prodan fluorescence spectra that change depending on the phase state of the PC bilayers, are explained. By using this bilayer imaging technique, the effects of the following influencing factors on the packing state of the PC molecules in the bilayer are considered, and the technique reveals that a slight difference in these tors remarkably affects the bilayer packing: (1) acyl chain length, (2) vesicle size, and (3) chain asymmetry. Furthermore, the technique also clarifies the formation of the lamellar crystal phase, as it is usually difficult to study the packing of the phase of the PC bilayer at low temperature and/or high pressure." @default.
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- W3017360034 date "2016-09-19" @default.
- W3017360034 modified "2023-10-14" @default.
- W3017360034 title "Bilayer Imaging of Phosphatidylcholines by High-Pressure Fluorometry" @default.
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- W3017360034 doi "https://doi.org/10.1002/9781119075691.ch70" @default.
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