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• W3017597105 abstract "ABSTRACT Sarcoplasmic reticulum (SR) Ca 2+ -ATPase (SERCA) and phospholamban (PLB) are essential for intracellular Ca 2+ transport in myocytes. Ca 2+ -dependent activation of SERCA–PLB provides a rheostat function that regulates cytosolic and SR Ca 2+ levels. While experimental and computational studies alone have led to a greater insight into the mechanisms for SERCA–PLB regulation, the structural changes induced by Ca 2+ binding and how those are communicated to couple enzymatic activity with active transport remain poorly understood. Therefore, we have performed atomistic simulations totaling 32.7 μs and cell-based intramolecular fluorescence resonance energy transfer (FRET) experiments to determine structural changes of PLB-bound SERCA in response to Ca 2+ binding. Complementary simulations and experiments showed structural disorder underlies PLB inhibition of SERCA, and Ca 2+ binding is sufficient to shift the protein population toward a structurally ordered state of the complex. This structural transition results in a redistribution of structural states toward a partially closed conformation of SERCA’s cytosolic headpiece. Closure is accompanied by functional interactions between the N-domain β5-β6 loop and the A-domain. Regulation of these key structural elements indicate that Ca 2+ is a critical mediator of allosteric signaling that dictates structural changes and motions that pre-activate SERCA–PLB. These findings provide direct support that dynamically driven protein allostery underlies PLB regulation of SERCA. These functional insights at unprecedented spatiotemporal resolution suggest a general modular architecture mechanism for dynamic regulation of the SERCA–PLB complex. Understanding these mechanisms is of paramount importance to guide therapeutic modulation of SERCA and other evolutionarily related ion-motive ATPases." @default.
• W3017597105 created "2020-05-01" @default.
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• W3017597105 date "2020-04-27" @default.
• W3017597105 modified "2023-10-16" @default.
• W3017597105 title "Dynamics-driven allostery underlies pre-activation of the regulatory Ca2+-ATPase/phospholamban complex" @default.
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• W3017597105 doi "https://doi.org/10.1101/2020.04.26.062299" @default.
• W3017597105 hasPublicationYear "2020" @default.
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