Matches in SemOpenAlex for { <https://semopenalex.org/work/W3018069874> ?p ?o ?g. }
- W3018069874 endingPage "23" @default.
- W3018069874 startingPage "17" @default.
- W3018069874 abstract "Hyperthermophilic xylanases play a critical role in bioconversion from xylan to sugar in the process of biomass utilization. The discovery of new or improvement of existing xylanases based on directed evolution is expected to be an effective approach to meet the increasing demand of thermostable xylanases. In this work, a xylanase B gene (CTN_0623) from Thermotoga neapolitana (Tne) was cloned and xylanase B (herein named TnexlnB) was solubly expressed in E. coli with a high amount using a heat shock vector pHsh. TnexlnB showed the highest endo-β-1,4-xylan hydrolase activity at 75 °C and pH 6.0. Additionally, 1 mM Mg2+, Ba2+ and Ca2+ improved the activity of TnexlnB by 31%, 37%, and 53%, respectively. The optimal temperature reached 85 °C by site-directed mutation at the last three helices of TnexlnB. Km and Vmax towards birchwood xylan were determined for both wide type and the best mutant, as follow: 1.09 mg/mL, 191.76 U/mg and 0.29 mg/mL, 376.42 U/mg, respectively. Further characterization highlighted good thermal stability (>80% of enzymatic activity after 1 h at 90 °C) for the best mutant, which made this enzyme suitable for biomass degradation at high temperature." @default.
- W3018069874 created "2020-05-01" @default.
- W3018069874 creator A5006229768 @default.
- W3018069874 creator A5033530426 @default.
- W3018069874 creator A5051931459 @default.
- W3018069874 creator A5052357648 @default.
- W3018069874 creator A5067770341 @default.
- W3018069874 date "2020-08-01" @default.
- W3018069874 modified "2023-10-18" @default.
- W3018069874 title "Efficient xylan-to-sugar biotransformation using an engineered xylanase in hyperthermic environment" @default.
- W3018069874 cites W1555038940 @default.
- W3018069874 cites W1965033962 @default.
- W3018069874 cites W1968704342 @default.
- W3018069874 cites W1970756887 @default.
- W3018069874 cites W1982784398 @default.
- W3018069874 cites W1985819009 @default.
- W3018069874 cites W2003309849 @default.
- W3018069874 cites W2014012537 @default.
- W3018069874 cites W2033616796 @default.
- W3018069874 cites W2036532473 @default.
- W3018069874 cites W2042181696 @default.
- W3018069874 cites W2047329045 @default.
- W3018069874 cites W2057716319 @default.
- W3018069874 cites W2071013400 @default.
- W3018069874 cites W2071594728 @default.
- W3018069874 cites W2088366138 @default.
- W3018069874 cites W2088921357 @default.
- W3018069874 cites W2092769046 @default.
- W3018069874 cites W2094201044 @default.
- W3018069874 cites W2094931921 @default.
- W3018069874 cites W2122559203 @default.
- W3018069874 cites W2135123110 @default.
- W3018069874 cites W2141913044 @default.
- W3018069874 cites W2152756549 @default.
- W3018069874 cites W2165947856 @default.
- W3018069874 cites W2166726458 @default.
- W3018069874 cites W2168974130 @default.
- W3018069874 cites W2265133336 @default.
- W3018069874 cites W2469325335 @default.
- W3018069874 cites W2491804579 @default.
- W3018069874 cites W2789564237 @default.
- W3018069874 cites W2790183111 @default.
- W3018069874 cites W2793793056 @default.
- W3018069874 cites W2885210981 @default.
- W3018069874 cites W2908867903 @default.
- W3018069874 cites W2924335595 @default.
- W3018069874 cites W2937412586 @default.
- W3018069874 cites W2964848374 @default.
- W3018069874 cites W4253164051 @default.
- W3018069874 doi "https://doi.org/10.1016/j.ijbiomac.2020.04.145" @default.
- W3018069874 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32335116" @default.
- W3018069874 hasPublicationYear "2020" @default.
- W3018069874 type Work @default.
- W3018069874 sameAs 3018069874 @default.
- W3018069874 citedByCount "6" @default.
- W3018069874 countsByYear W30180698742021 @default.
- W3018069874 countsByYear W30180698742022 @default.
- W3018069874 countsByYear W30180698742023 @default.
- W3018069874 crossrefType "journal-article" @default.
- W3018069874 hasAuthorship W3018069874A5006229768 @default.
- W3018069874 hasAuthorship W3018069874A5033530426 @default.
- W3018069874 hasAuthorship W3018069874A5051931459 @default.
- W3018069874 hasAuthorship W3018069874A5052357648 @default.
- W3018069874 hasAuthorship W3018069874A5067770341 @default.
- W3018069874 hasConcept C100544194 @default.
- W3018069874 hasConcept C104317684 @default.
- W3018069874 hasConcept C115540264 @default.
- W3018069874 hasConcept C116174643 @default.
- W3018069874 hasConcept C143065580 @default.
- W3018069874 hasConcept C181199279 @default.
- W3018069874 hasConcept C185592680 @default.
- W3018069874 hasConcept C196032511 @default.
- W3018069874 hasConcept C2775859485 @default.
- W3018069874 hasConcept C2776519085 @default.
- W3018069874 hasConcept C2777002520 @default.
- W3018069874 hasConcept C2777108408 @default.
- W3018069874 hasConcept C2780074830 @default.
- W3018069874 hasConcept C2781216036 @default.
- W3018069874 hasConcept C27881333 @default.
- W3018069874 hasConcept C31903555 @default.
- W3018069874 hasConcept C547475151 @default.
- W3018069874 hasConcept C55493867 @default.
- W3018069874 hasConcept C6557445 @default.
- W3018069874 hasConcept C86803240 @default.
- W3018069874 hasConceptScore W3018069874C100544194 @default.
- W3018069874 hasConceptScore W3018069874C104317684 @default.
- W3018069874 hasConceptScore W3018069874C115540264 @default.
- W3018069874 hasConceptScore W3018069874C116174643 @default.
- W3018069874 hasConceptScore W3018069874C143065580 @default.
- W3018069874 hasConceptScore W3018069874C181199279 @default.
- W3018069874 hasConceptScore W3018069874C185592680 @default.
- W3018069874 hasConceptScore W3018069874C196032511 @default.
- W3018069874 hasConceptScore W3018069874C2775859485 @default.
- W3018069874 hasConceptScore W3018069874C2776519085 @default.
- W3018069874 hasConceptScore W3018069874C2777002520 @default.
- W3018069874 hasConceptScore W3018069874C2777108408 @default.
- W3018069874 hasConceptScore W3018069874C2780074830 @default.
- W3018069874 hasConceptScore W3018069874C2781216036 @default.