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• W3018365404 abstract "Alternative polyadenylation (APA) occurs in the process of mRNA maturation by adding a poly(A) tail at different locations, resulting increased diversity of mRNA isoforms and contributing to the complexity of gene regulatory network. Benefit from the development of high-throughput sequencing technologies, we could now delineate APA profiles of transcriptomes at an unprecedented pace. Especially the single cell RNA sequencing (scRNA-seq) technologies provide us opportunities to interrogate biological details of diverse and rare cell types. Despite increasing evidence showing that APA is involved in the cell type-specific regulation and function, efficient and specific laboratory methods for capturing poly(A) sites at single cell resolution are underdeveloped to date. In this review, we summarize existing experimental and computational methods for the identification of APA dynamics from diverse single cell types. A future perspective is also provided." @default.
• W3018365404 created "2020-05-01" @default.
• W3018365404 creator A5011758500 @default.
• W3018365404 creator A5032396845 @default.
• W3018365404 creator A5082836860 @default.
• W3018365404 date "2020-01-01" @default.
• W3018365404 modified "2023-10-14" @default.
• W3018365404 title "Discovery of alternative polyadenylation dynamics from single cell types" @default.
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• W3018365404 doi "https://doi.org/10.1016/j.csbj.2020.04.009" @default.
• W3018365404 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7200215" @default.
• W3018365404 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32382395" @default.
• W3018365404 hasPublicationYear "2020" @default.
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