FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3018804618> ?p ?o ?g. }

• W3018804618 abstract "Abstract Global poliovirus surveillance involves virus isolation from stool and environmental samples, intratypic differential (ITD) by PCR and sequencing of the VP1 region to distinguish vaccine (Sabin), vaccine-derived and wild-type polioviruses and ensure an appropriate response. This cell-culture algorithm takes 2-3 weeks on average between sample receipt and sequencing. Direct detection of viral RNA using PCR allows faster detection but has traditionally faced challenges related to poor sensitivity and difficulties in sequencing common samples containing poliovirus and enterovirus mixtures. We present a nested PCR and nanopore sequencing protocol that allows rapid (<3 days) and sensitive direct detection and sequencing of polioviruses in stool and environmental samples. We developed barcoded primers and a real-time analysis platform that generate accurate VP1 consensus sequences from multiplexed samples. The sensitivity and specificity compared with cell-culture were 90.9% (95% Confidence Interval: 75.7-98.1%) and 99.2% (95.5-100.0%) for wild-type 1 poliovirus, 92.5% (79.6-98.4%) and 98.7% (95.4-99.8%) for vaccine and vaccine-derived serotype 2 poliovirus, and 88.3% (81.2-93.5%) and 93.2% (88.6-96.3%) for Sabin 1 and 3 poliovirus alone or in mixtures when tested on 155 stool samples in Pakistan. Variant analysis of sequencing reads also allowed identification of polioviruses and enteroviruses in artificial mixtures and was able to distinguish complex mixtures of polioviruses in environmental samples. The median identity of consensus nanopore sequences with Sanger or Illumina sequences from the same samples was >99.9%. This novel method shows promise as a faster and safer alternative to cell-culture for the detection and real-time sequencing of polioviruses in stool and environmental samples." @default.
• W3018804618 created "2020-05-01" @default.
• W3018804618 creator A5009086499 @default.
• W3018804618 creator A5010655685 @default.
• W3018804618 creator A5012480083 @default.
• W3018804618 creator A5017687464 @default.
• W3018804618 creator A5018458695 @default.
• W3018804618 creator A5019654788 @default.
• W3018804618 creator A5030618085 @default.
• W3018804618 creator A5037647464 @default.
• W3018804618 creator A5039700341 @default.
• W3018804618 creator A5041022330 @default.
• W3018804618 creator A5043177251 @default.
• W3018804618 creator A5051956314 @default.
• W3018804618 creator A5052197488 @default.
• W3018804618 creator A5057259613 @default.
• W3018804618 creator A5064124046 @default.
• W3018804618 creator A5064783824 @default.
• W3018804618 creator A5065851931 @default.
• W3018804618 creator A5069412319 @default.
• W3018804618 creator A5072272515 @default.
• W3018804618 creator A5081145350 @default.
• W3018804618 creator A5082210981 @default.
• W3018804618 creator A5083534383 @default.
• W3018804618 creator A5085208708 @default.
• W3018804618 creator A5086483348 @default.
• W3018804618 creator A5087299636 @default.
• W3018804618 creator A5090055868 @default.
• W3018804618 date "2020-04-28" @default.
• W3018804618 modified "2023-09-26" @default.
• W3018804618 title "Rapid and sensitive direct detection and identification of poliovirus from stool and environmental surveillance samples using nanopore sequencing" @default.
• W3018804618 cites W2021210077 @default.
• W3018804618 cites W2023992636 @default.
• W3018804618 cites W2027597182 @default.
• W3018804618 cites W2037110820 @default.
• W3018804618 cites W2102060315 @default.
• W3018804618 cites W2103497166 @default.
• W3018804618 cites W2104148453 @default.
• W3018804618 cites W2107939515 @default.
• W3018804618 cites W2110417468 @default.
• W3018804618 cites W2117801354 @default.
• W3018804618 cites W2125488301 @default.
• W3018804618 cites W2129812184 @default.
• W3018804618 cites W2144775551 @default.
• W3018804618 cites W2145645825 @default.
• W3018804618 cites W2160378127 @default.
• W3018804618 cites W2170287831 @default.
• W3018804618 cites W2194172909 @default.
• W3018804618 cites W2335682086 @default.
• W3018804618 cites W2513506562 @default.
• W3018804618 cites W2575750030 @default.
• W3018804618 cites W2598379945 @default.
• W3018804618 cites W2779437262 @default.
• W3018804618 cites W2799524357 @default.
• W3018804618 cites W2948253547 @default.
• W3018804618 cites W2982395627 @default.
• W3018804618 cites W4236236547 @default.
• W3018804618 doi "https://doi.org/10.1101/2020.04.27.053421" @default.
• W3018804618 hasPublicationYear "2020" @default.
• W3018804618 type Work @default.
• W3018804618 sameAs 3018804618 @default.
• W3018804618 citedByCount "3" @default.
• W3018804618 countsByYear W30188046182021 @default.
• W3018804618 crossrefType "posted-content" @default.
• W3018804618 hasAuthorship W3018804618A5009086499 @default.
• W3018804618 hasAuthorship W3018804618A5010655685 @default.
• W3018804618 hasAuthorship W3018804618A5012480083 @default.
• W3018804618 hasAuthorship W3018804618A5017687464 @default.
• W3018804618 hasAuthorship W3018804618A5018458695 @default.
• W3018804618 hasAuthorship W3018804618A5019654788 @default.
• W3018804618 hasAuthorship W3018804618A5030618085 @default.
• W3018804618 hasAuthorship W3018804618A5037647464 @default.
• W3018804618 hasAuthorship W3018804618A5039700341 @default.
• W3018804618 hasAuthorship W3018804618A5041022330 @default.
• W3018804618 hasAuthorship W3018804618A5043177251 @default.
• W3018804618 hasAuthorship W3018804618A5051956314 @default.
• W3018804618 hasAuthorship W3018804618A5052197488 @default.
• W3018804618 hasAuthorship W3018804618A5057259613 @default.
• W3018804618 hasAuthorship W3018804618A5064124046 @default.
• W3018804618 hasAuthorship W3018804618A5064783824 @default.
• W3018804618 hasAuthorship W3018804618A5065851931 @default.
• W3018804618 hasAuthorship W3018804618A5069412319 @default.
• W3018804618 hasAuthorship W3018804618A5072272515 @default.
• W3018804618 hasAuthorship W3018804618A5081145350 @default.
• W3018804618 hasAuthorship W3018804618A5082210981 @default.
• W3018804618 hasAuthorship W3018804618A5083534383 @default.
• W3018804618 hasAuthorship W3018804618A5085208708 @default.
• W3018804618 hasAuthorship W3018804618A5086483348 @default.
• W3018804618 hasAuthorship W3018804618A5087299636 @default.
• W3018804618 hasAuthorship W3018804618A5090055868 @default.
• W3018804618 hasBestOaLocation W30188046181 @default.
• W3018804618 hasConcept C10389963 @default.
• W3018804618 hasConcept C104317684 @default.
• W3018804618 hasConcept C126513998 @default.
• W3018804618 hasConcept C159047783 @default.
• W3018804618 hasConcept C199360897 @default.
• W3018804618 hasConcept C2522874641 @default.
• W3018804618 hasConcept C2777888124 @default.
• W3018804618 hasConcept C2779631682 @default.
• W3018804618 hasConcept C2781188995 @default.

• next