FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3019087673> ?p ?o ?g. }

• W3019087673 endingPage "1027" @default.
• W3019087673 startingPage "1027" @default.
• W3019087673 abstract "The homologs EpCAM and TROP2, which both interact with claudin-1 and claudin-7, are frequently coexpressed in epithelia including skin. Intestine uniquely expresses high levels of EpCAM but not TROP2. We previously identified EpCAM as a substrate of the membrane-anchored protease matriptase and linked HAI-2, matriptase, EpCAM and claudin-7 in a pathway that is pivotal for intestinal epithelial cells (IEC) homeostasis. Herein, we reveal that TROP2 is also a matriptase substrate. Matriptase cleaved TROP2 when purified recombinant proteins were mixed in vitro. TROP2, like EpCAM, was also cleaved after co-transfection of matriptase in 293T cells. Neither EpCAM nor TROP2 cleavage was promoted by protease-disabled matriptase or matriptase that harbored the ichthyosis-associated G827R mutation. We confirmed that EpCAM and TROP2 are both expressed in skin and detected cleavage of these proteins in human keratinocytes (HaCaT cells) after the physiologic inhibition of matriptase by HAI proteins was relieved by siRNA knockdown. Knockdown of EpCAM or TROP2 individually had only small effects on claudin-1 and claudin-7 levels, whereas elimination of both markedly diminished claudin levels. HAI-1 knockdown promoted EpCAM and TROP2 cleavage accompanied by reductions in claudins, whereas HAI-2 knockdown had little impact. Double knockdown of HAI-1 and HAI-2 induced nearly complete cleavage of EpCAM and TROP2 and drastic reductions of claudins. These effects were eliminated by concurrent matriptase knockdown. Decreases in claudin levels were also diminished by the lysosomal inhibitor chloroquine and cleaved EpCAM/TROP2 fragments accumulated preferentially. We demonstrate that TROP2 and EpCAM exhibit redundancies with regard to regulation of claudin metabolism and that an HAI, matriptase, EpCAM and claudin pathway analogous to what we described in IECs exists in keratinocytes. This study may offer insights into the mechanistic basis for matriptase dysregulation-induced ichthyosis." @default.
• W3019087673 created "2020-05-01" @default.
• W3019087673 creator A5033399543 @default.
• W3019087673 creator A5037651981 @default.
• W3019087673 creator A5051811907 @default.
• W3019087673 creator A5054588591 @default.
• W3019087673 creator A5087149828 @default.
• W3019087673 date "2020-04-21" @default.
• W3019087673 modified "2023-10-14" @default.
• W3019087673 title "Matriptase Cleaves EpCAM and TROP2 in Keratinocytes, Destabilizing Both Proteins and Associated Claudins" @default.
• W3019087673 cites W181487094 @default.
• W3019087673 cites W1969754678 @default.
• W3019087673 cites W1973834233 @default.
• W3019087673 cites W1981234066 @default.
• W3019087673 cites W1985544363 @default.
• W3019087673 cites W1986978780 @default.
• W3019087673 cites W1991346408 @default.
• W3019087673 cites W1991757994 @default.
• W3019087673 cites W1999561220 @default.
• W3019087673 cites W2003621291 @default.
• W3019087673 cites W2010072619 @default.
• W3019087673 cites W2014240063 @default.
• W3019087673 cites W2015734787 @default.
• W3019087673 cites W2016382201 @default.
• W3019087673 cites W2019284666 @default.
• W3019087673 cites W2020179673 @default.
• W3019087673 cites W2021179643 @default.
• W3019087673 cites W2028351310 @default.
• W3019087673 cites W2031904940 @default.
• W3019087673 cites W2032716405 @default.
• W3019087673 cites W2044689258 @default.
• W3019087673 cites W2053325230 @default.
• W3019087673 cites W2070882362 @default.
• W3019087673 cites W2071788821 @default.
• W3019087673 cites W2076401425 @default.
• W3019087673 cites W2093054586 @default.
• W3019087673 cites W2094468216 @default.
• W3019087673 cites W2099061795 @default.
• W3019087673 cites W2103100739 @default.
• W3019087673 cites W2103729492 @default.
• W3019087673 cites W2108038768 @default.
• W3019087673 cites W2114082590 @default.
• W3019087673 cites W2115573646 @default.
• W3019087673 cites W2132024007 @default.
• W3019087673 cites W2152463640 @default.
• W3019087673 cites W2156034391 @default.
• W3019087673 cites W2158264341 @default.
• W3019087673 cites W2158741313 @default.
• W3019087673 cites W2180671180 @default.
• W3019087673 cites W2188271125 @default.
• W3019087673 cites W2573789295 @default.
• W3019087673 cites W2790046231 @default.
• W3019087673 cites W2907543715 @default.
• W3019087673 cites W2974613403 @default.
• W3019087673 cites W2980826521 @default.
• W3019087673 doi "https://doi.org/10.3390/cells9041027" @default.
• W3019087673 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7226414" @default.
• W3019087673 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32326212" @default.
• W3019087673 hasPublicationYear "2020" @default.
• W3019087673 type Work @default.
• W3019087673 sameAs 3019087673 @default.
• W3019087673 citedByCount "21" @default.
• W3019087673 countsByYear W30190876732020 @default.
• W3019087673 countsByYear W30190876732021 @default.
• W3019087673 countsByYear W30190876732022 @default.
• W3019087673 countsByYear W30190876732023 @default.
• W3019087673 crossrefType "journal-article" @default.
• W3019087673 hasAuthorship W3019087673A5033399543 @default.
• W3019087673 hasAuthorship W3019087673A5037651981 @default.
• W3019087673 hasAuthorship W3019087673A5051811907 @default.
• W3019087673 hasAuthorship W3019087673A5054588591 @default.
• W3019087673 hasAuthorship W3019087673A5087149828 @default.
• W3019087673 hasBestOaLocation W30190876731 @default.
• W3019087673 hasConcept C104317684 @default.
• W3019087673 hasConcept C1491633281 @default.
• W3019087673 hasConcept C153911025 @default.
• W3019087673 hasConcept C173396325 @default.
• W3019087673 hasConcept C174510640 @default.
• W3019087673 hasConcept C177779419 @default.
• W3019087673 hasConcept C185592680 @default.
• W3019087673 hasConcept C2776677690 @default.
• W3019087673 hasConcept C502942594 @default.
• W3019087673 hasConcept C51872919 @default.
• W3019087673 hasConcept C54009773 @default.
• W3019087673 hasConcept C55493867 @default.
• W3019087673 hasConcept C86803240 @default.
• W3019087673 hasConcept C95444343 @default.
• W3019087673 hasConceptScore W3019087673C104317684 @default.
• W3019087673 hasConceptScore W3019087673C1491633281 @default.
• W3019087673 hasConceptScore W3019087673C153911025 @default.
• W3019087673 hasConceptScore W3019087673C173396325 @default.
• W3019087673 hasConceptScore W3019087673C174510640 @default.
• W3019087673 hasConceptScore W3019087673C177779419 @default.
• W3019087673 hasConceptScore W3019087673C185592680 @default.
• W3019087673 hasConceptScore W3019087673C2776677690 @default.
• W3019087673 hasConceptScore W3019087673C502942594 @default.
• W3019087673 hasConceptScore W3019087673C51872919 @default.
• W3019087673 hasConceptScore W3019087673C54009773 @default.

• next