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• W3019249497 abstract "• A new DNAzyme “turn on” visual method to quantify PCR products is presented. • The linker enables the single-double strands transition and G4 controlled release. • Visual analysis is conducted through the G4-DNAzyme colorimetric reaction. • The strategy is simple and signal amplification can be realized within a few minutes. • The universal primers can be applied in the detection of other target substances. Here, we constructed a fast, universal, “turn-on” biosensor for the highly sensitive visual detection of Salmonella based on luminescent DNAzyme and a universal blocking linker Super Polymerase Chain Reaction (S-PCR). The primer in this biosensor was specially designed. The G-quadruplex sequence is attached to the 5’ end of the primer by a blocking linker and is blocked in the stem part. The S-PCR amplification releases the G-quadruplex, which is incubated with hemin to form DNAzyme to exert peroxide-like activity. The visible colored products are generated by the addition of 3,3′,5,5′-Tetramethylbenzidine. Detection sensitivity is high and, even when the concentration of the Salmonella genome in a sample is as low as 1.5 copies/µL, this color change can be seen with the naked eyes. Moreover, this method is simple and fast, as the S-PCR can be completed in less than 10 min using the newly built equipment and without the need for large instruments." @default.
• W3019249497 created "2020-05-01" @default.
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• W3019249497 date "2020-09-01" @default.
• W3019249497 modified "2023-10-16" @default.
• W3019249497 title "Luminescent DNAzyme and universal blocking linker Super Polymerase Chain Reaction visual biosensor for the detection of Salmonella" @default.
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• W3019249497 doi "https://doi.org/10.1016/j.foodchem.2020.126859" @default.
• W3019249497 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32344343" @default.
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