Matches in SemOpenAlex for { <https://semopenalex.org/work/W3019287920> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W3019287920 endingPage "16230" @default.
- W3019287920 startingPage "16221" @default.
- W3019287920 abstract "The main aim of the present study was the synthesis of an oligonucleotide-based material with high chemical stability, repeatability and specificity to complementary oligonucleotides. The oligonucleotides were attached to a silica gel surface modified with amino acids during one-step synthesis. The amount of the oligonucleotides immobilized on the support surface had an impact on adsorption effectiveness, due to steric interference. The adsorption capacity corresponds to 4.7 μg of complementary oligonucleotide per 1 mg of material, which reflects 50% of immobilized oligonucleotides. The presented results contain comprehensive studies on hybridization and release of fully complementary, partially complementary, non-complementary and antisense oligonucleotides from the newly synthesized adsorbent. The salt concentration and time period were the most influential parameters in the case of adsorption, while high temperature and low salt content were indispensable for effective desorption. Selectivity studies revealed that the adsorption percentage increases with the decreasing number of base mismatches. Consequently, the desorption of low complementarity oligonucleotides was always greater in comparison with the fully complementary sequence. Furthermore, it was shown that oligonucleotide-based materials may be successfully used for the extraction of antisense oligonucleotides and their metabolites from serum samples with recoveries ranging between 65 and 73%." @default.
- W3019287920 created "2020-05-01" @default.
- W3019287920 creator A5021949137 @default.
- W3019287920 creator A5036089720 @default.
- W3019287920 creator A5055860468 @default.
- W3019287920 creator A5056759324 @default.
- W3019287920 creator A5079164571 @default.
- W3019287920 date "2020-01-01" @default.
- W3019287920 modified "2023-09-25" @default.
- W3019287920 title "Attachment of hybridizable oligonucleotides to a silica support and its application for selective extraction of unmodified and antisense oligonucleotides from serum samples" @default.
- W3019287920 cites W1965915039 @default.
- W3019287920 cites W1975188059 @default.
- W3019287920 cites W1976381274 @default.
- W3019287920 cites W1995748995 @default.
- W3019287920 cites W1996706226 @default.
- W3019287920 cites W2061805552 @default.
- W3019287920 cites W206372758 @default.
- W3019287920 cites W2078820160 @default.
- W3019287920 cites W2101669631 @default.
- W3019287920 cites W2153632158 @default.
- W3019287920 cites W2333379319 @default.
- W3019287920 cites W2507983288 @default.
- W3019287920 cites W2525906082 @default.
- W3019287920 cites W2789580484 @default.
- W3019287920 cites W2895155188 @default.
- W3019287920 cites W2919875302 @default.
- W3019287920 cites W2946112378 @default.
- W3019287920 cites W2964042927 @default.
- W3019287920 cites W2995050875 @default.
- W3019287920 doi "https://doi.org/10.1039/d0ra01620a" @default.
- W3019287920 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35498856" @default.
- W3019287920 hasPublicationYear "2020" @default.
- W3019287920 type Work @default.
- W3019287920 sameAs 3019287920 @default.
- W3019287920 citedByCount "6" @default.
- W3019287920 countsByYear W30192879202021 @default.
- W3019287920 countsByYear W30192879202022 @default.
- W3019287920 countsByYear W30192879202023 @default.
- W3019287920 crossrefType "journal-article" @default.
- W3019287920 hasAuthorship W3019287920A5021949137 @default.
- W3019287920 hasAuthorship W3019287920A5036089720 @default.
- W3019287920 hasAuthorship W3019287920A5055860468 @default.
- W3019287920 hasAuthorship W3019287920A5056759324 @default.
- W3019287920 hasAuthorship W3019287920A5079164571 @default.
- W3019287920 hasBestOaLocation W30192879201 @default.
- W3019287920 hasConcept C118792377 @default.
- W3019287920 hasConcept C129312508 @default.
- W3019287920 hasConcept C150394285 @default.
- W3019287920 hasConcept C161790260 @default.
- W3019287920 hasConcept C162711632 @default.
- W3019287920 hasConcept C178790620 @default.
- W3019287920 hasConcept C185592680 @default.
- W3019287920 hasConcept C21951064 @default.
- W3019287920 hasConcept C43617362 @default.
- W3019287920 hasConcept C552990157 @default.
- W3019287920 hasConcept C55493867 @default.
- W3019287920 hasConceptScore W3019287920C118792377 @default.
- W3019287920 hasConceptScore W3019287920C129312508 @default.
- W3019287920 hasConceptScore W3019287920C150394285 @default.
- W3019287920 hasConceptScore W3019287920C161790260 @default.
- W3019287920 hasConceptScore W3019287920C162711632 @default.
- W3019287920 hasConceptScore W3019287920C178790620 @default.
- W3019287920 hasConceptScore W3019287920C185592680 @default.
- W3019287920 hasConceptScore W3019287920C21951064 @default.
- W3019287920 hasConceptScore W3019287920C43617362 @default.
- W3019287920 hasConceptScore W3019287920C552990157 @default.
- W3019287920 hasConceptScore W3019287920C55493867 @default.
- W3019287920 hasFunder F4320322511 @default.
- W3019287920 hasIssue "28" @default.
- W3019287920 hasLocation W30192879201 @default.
- W3019287920 hasLocation W30192879202 @default.
- W3019287920 hasLocation W30192879203 @default.
- W3019287920 hasOpenAccess W3019287920 @default.
- W3019287920 hasPrimaryLocation W30192879201 @default.
- W3019287920 hasRelatedWork W1996358191 @default.
- W3019287920 hasRelatedWork W2047714983 @default.
- W3019287920 hasRelatedWork W2080948544 @default.
- W3019287920 hasRelatedWork W2100688936 @default.
- W3019287920 hasRelatedWork W2367655658 @default.
- W3019287920 hasRelatedWork W2378008466 @default.
- W3019287920 hasRelatedWork W2380551708 @default.
- W3019287920 hasRelatedWork W2384918352 @default.
- W3019287920 hasRelatedWork W2957460539 @default.
- W3019287920 hasRelatedWork W3208502522 @default.
- W3019287920 hasVolume "10" @default.
- W3019287920 isParatext "false" @default.
- W3019287920 isRetracted "false" @default.
- W3019287920 magId "3019287920" @default.
- W3019287920 workType "article" @default.