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• W3019315834 abstract "Cellulose nanocrystals (CNCs), having a high specific surface area and versatile surface chemistry, provide considerable potential to interact by various mechanisms with enzymes for nano-immobilization purposes. However, engineering chemically safe CNCs, suitable for edible administrations, presents a significant challenge. A reliable carboxylate form of H-CNCs was formed using H2O2 oxidation of softwood pulp under mild thermal conditions. Negatively charged carboxyl groups (∼0.9 mmol g–1) played a key role in lysozyme immobilization via electrostatic interactions and covalent linkages, as evidenced by Fourier transform infrared and 13C cross-polarization magic angle spinning nuclear magnetic resonance spectroscopies. Adsorption isotherms showed a high loading capacity of H-CNCs (∼240 mg g–1), and fitting the data to the Langmuir model confirmed monolayer coverage of lysozyme on their surface. Using a non-toxic coupling agent, 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride, lysozyme-conjugated H-CNCs were developed with an immobilization yield of ∼65% and relative catalytic activity of ∼60%, similar to lysozyme adsorption on H-CNCs. These H-CNC–lysozyme nanohybrids, rationally processed via safe and green strategies, are specifically exploitable as catalytically active emulsifiers in food and pharmaceutical sectors." @default.
• W3019315834 created "2020-05-01" @default.
• W3019315834 creator A5029940830 @default.
• W3019315834 creator A5046135600 @default.
• W3019315834 date "2020-04-24" @default.
• W3019315834 modified "2023-10-16" @default.
• W3019315834 title "Carboxylated Cellulose Nanocrystals Developed by Cu-Assisted H₂O₂ Oxidation as Green Nanocarriers for Efficient Lysozyme Immobilization" @default.
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• W3019315834 doi "https://doi.org/10.1021/acs.jafc.0c00538" @default.
• W3019315834 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32330021" @default.
• W3019315834 hasPublicationYear "2020" @default.
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