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• W3019553544 endingPage "102874" @default.
• W3019553544 startingPage "102874" @default.
• W3019553544 abstract "The zebrafish Weberian apparatus is an emerging model for human conductive hearing system. Their Weberian apparatus comprises minute bones and ligamentary links, and conducts sound pressure transmission from the gas bladder to inner ear through four pairs of Weberian ossicles along the vertebral column. We herein present a methodological study using MicroCT to image the Weberian apparatus for biomechanical and morphological analysis. The aim of this work is to evaluate computational models generated from multiple MicroCT scans with different parameters, to identify the most feasible scan combination for practical (minimized scan time) yet accurate (relative to highest resolution) biomechanical simulations. We segmented and created 3D models from CT scan image stacks at 4.64 μm, 5.05 μm, 9.30 μm and 13.08 μm voxel resolutions, respectively. Then, we used geometric morphometrics analysis to quantify inter-model shape differences, as well as a series of finite element modal and harmonic analyses to simulate auditory signal vibrations. Relative to the highest resolution and most accurate model, the Model 9.30 is closest in overall geometry and biomechanical behavior of all lower resolution models. The differences in resolution and quality of the CT substantially affect the segmentation and reconstruction process of the three-dimensional model of the ossicles, and the subsequent analyses. We conclude that scan voxel resolution is a key factor influencing outcomes of biomechanical simulations of delicate and minute structures, especially when studying the harmonic response of minute ossicles connected by ligaments using finite element modeling. Furthermore, contrast variations in CT images as determined by x-ray power and scan speed, also affect fidelity in 3D models and simulation outcomes." @default.
• W3019553544 created "2020-05-01" @default.
• W3019553544 creator A5050365463 @default.
• W3019553544 creator A5080125836 @default.
• W3019553544 date "2020-08-01" @default.
• W3019553544 modified "2023-10-18" @default.
• W3019553544 title "Evaluating fidelity of CT based 3D models for Zebrafish conductive hearing system" @default.
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• W3019553544 doi "https://doi.org/10.1016/j.micron.2020.102874" @default.
• W3019553544 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32388237" @default.
• W3019553544 hasPublicationYear "2020" @default.
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