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• W3019832619 abstract "Introduction In recent years, a large number of fixation and preservation liquids have been proposed in the search for alternative methods of body preservation. Two processes occur postmortem: putrefaction with bacterial action on body tissues and autolysis with decomposition of the body by endogenous substances. Thus, we intend to present a new formula that allows the body to be preserved with its morphological and mobility characteristics similar to the fresh cadaver, but ensuring its fixation and conservation over time, maintaining at room temperature. Material and Method We use a donated 69-years-old female body, was embalmed using a new solution based on Baptista et al. (1986) with embalming pump. The formula used was: alcohol 1000 ml; phenol 1000 g; glycerin 1000 ml; sodium nitrate 1000 g; formaldehyde 2000 ml; distilled water 14000 ml. Arterial accesses and complementary injections of formaldehyde were performed in muscles limbs and cranial cavity. The cadaver was maintained at room temperature, ranging from 20.1 to 32.6 °C (68.18–90.68 °F) and the humidity (41–94) in body bag covered with blackout sheet. Samples of tissues (skin and subcutaneous tissue, rectus abdominis muscle, quadrate lobe of liver, transverse colon and brain) were collected after 130, 150, 185, 360 and 512 days of embalming and submitted to histological staining (HE). For the microbiological analysis we used samples after 185 and 360 days and seeded on agar plates (blood, chocolate, and Sabouraud) and placed in liquid medium (TSB) and incubated for 72 hours (35–37 °C). Results This method resulted in soft and flexible cadavers as can be seen at elbow joint (Figs. 1a, 1b) with almost natural colors (Fig. 2). Histological analysis indicated the preservation of tissue and cellular structure in the different samples examined, even after 512 days post-embalming: skin and subcutaneous tissue (Fig. 3); muscle (Fig. 4); liver (Fig. 5) and brain (Fig 6). The cultures were negative for growth of microorganisms in both analysis (after 185 and 360 days). Discussion The cadaver preservation technique presented has low cost and easy execution, being effective in maintaining the morphological characteristics of the anatomical specimen for an extended period of time. Conclusion It presents as an alternative to anatomical teaching and research centers that have to deal with the toxicity of fixation products and the costs of obtaining and maintaining anatomical specimens. Figure 1Open in figure viewerPowerPoint Demonstration of the cadaver elbow joint flexibility after 512 days post embalming; Figure 2Open in figure viewerPowerPoint Aspect of the coloration of the tissues of the abdominal organs, 360 days post embalming. Figure 3Open in figure viewerPowerPoint Histological sections after 512 days of fixation of skin and subcutaneous tissue, muscle, liver and brain, respectively. HE. 200x." @default.
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• W3019832619 date "2020-04-01" @default.
• W3019832619 modified "2023-09-23" @default.
• W3019832619 title "Development of a new technique of embalming and preservation until almost one year at room temperature and dry storage: histological and microbiological analysis" @default.
• W3019832619 doi "https://doi.org/10.1096/fasebj.2020.34.s1.06763" @default.
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