FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3019833029> ?p ?o ?g. }

• W3019833029 endingPage "S198" @default.
• W3019833029 startingPage "S198" @default.
• W3019833029 abstract "Purpose: Synovium-derived mesenchymal stem cells (MSCs) have a high proliferative and chondrogenic potential in vitro and the intra-articular transplantation of MSCs have been reported to be useful for repair of articular cartilage and meniscus. Although synovium-derived MSCs have been explored to become a new treatment for the regeneration cartilage, synovium-resident MSCs have not been identified in native joints because of a lack of specific markers. Synovial hyperplasia is common in joint injuries and arthritis, but very little is known about the identity of the synovial cells that proliferate following injury and arthritis. A previous study using a articular cartilage injury mouse model revealed that some synovial cells were quiescent and slow-cycling like adult stem cells in other tissues, showed proliferation after injury and were identified as MSCs. However, the location and kinetics of proliferating synovial cells in an arthritis model are unknown. In this study, we examined the location and cell kinetics of proliferating synovial cells in a rat knee arthritis model. Methods: We created an arthritis model by intra-articular injection of low-dose monoiodoacetic acid (MIA, 0.2mg/30μl) to knees of 8 weeks old male Wistar rats (day 0). We labeled proliferating(dividing) cells with two different artificial nucleoside analogs with intra-articular injection, chlorodeoxyuridine (CldU, 0.3mg/30μl) at day 1,2 and iododeoxyuridine (IdU, 0.3mg/30μl) at day 3,4 respectively (The experimental scheme was showed at left side in upper row of Figure 1). At day 5 and 14, rats were euthanized by CO2 gas. The whole knee joints were removed and embedded in paraffin after fixation in 4% paraformaldehyde for 1 week and decalcification in 20% EDTA for 3 weeks. 5 micrometer thick sections were made and stained with hematoxylin and eosin (HE) for histological assessment. Immunofluorescence was also performed for assessment of labeling cells with rat anti-BrdU antibody for CldU and mouse anti-BrdU antibody for IdU which were adopted in other studies using these nucleoside analogs. Reverse administration model of CldU and IdU was also created. Rats were injected IdU at day 1,2 and CldU at day 3,4 before MIA injection at day 0 (The scheme was showed at right side in upper row of Figure 1). At day 5, immunofluorescent assessment was performed. To support these labeling methods, the experiment about half-life of CldU and IdU was investigated. Rats received CldU or IdU with intra-articular injection to knees 1 day before administration of MIA and immunofluorescent assessment was perfomerd at day 5 (showed in lower row of Figure 1). Results: First of all, the experiment about half-life of CldU and IdU showed that immunofluorescence did not detect both nucleoside analogs in the whole knee joint at day 5 (data not shown). It suggests that CldU and IdU are not effective and incorporated to cells at 1 day after injection. At day 5, synovial hyperplasia was observed through cell proliferation (HE stained sections were showed in Figure 2). CldU-positive cells (which were considered to have proliferated and incorporated CldU from day 1 to day 3) were in all range of synovium and some of them also in infrapatellar fat pad, but most IdU-positive cells (which were considered to have proliferated from day 3 to 5) were in superficial layer (Immunofluorescence sections were showed at the upper row in Figure 3). At day14, synovial hyperplasia weakened and the number of CldU- and IdU-positive cells decreased and the distribution of them was not distinct (data not shown). Reverse administration model showed a reverse result in that IdU-positive cells were detected from superficial layer to deeper layer of synovium and most CldU-positive cells were in superficial layer (showed at the lower row of Figure 3). Conclusions: In this model, synovial cells proliferated from superficial layer and widened toward deeper layer chronologically. It suggests that the origin of cells to sustain synovial hyperplasia is the superficial layer of synovium, not sub-lining tissue and migrating cells from extra-joint." @default.
• W3019833029 created "2020-05-01" @default.
• W3019833029 creator A5010801196 @default.
• W3019833029 creator A5034273023 @default.
• W3019833029 creator A5036019652 @default.
• W3019833029 creator A5039180718 @default.
• W3019833029 creator A5051157247 @default.
• W3019833029 creator A5059471349 @default.
• W3019833029 creator A5076671209 @default.
• W3019833029 creator A5078723577 @default.
• W3019833029 creator A5085591173 @default.
• W3019833029 date "2020-04-01" @default.
• W3019833029 modified "2023-09-27" @default.
• W3019833029 title "Synovial superficial cells proliferate toward deeper layer of synovium in a rat monoiodoacetic acid induced arthritis model" @default.
• W3019833029 doi "https://doi.org/10.1016/j.joca.2020.02.322" @default.
• W3019833029 hasPublicationYear "2020" @default.
• W3019833029 type Work @default.
• W3019833029 sameAs 3019833029 @default.
• W3019833029 citedByCount "0" @default.
• W3019833029 crossrefType "journal-article" @default.
• W3019833029 hasAuthorship W3019833029A5010801196 @default.
• W3019833029 hasAuthorship W3019833029A5034273023 @default.
• W3019833029 hasAuthorship W3019833029A5036019652 @default.
• W3019833029 hasAuthorship W3019833029A5039180718 @default.
• W3019833029 hasAuthorship W3019833029A5051157247 @default.
• W3019833029 hasAuthorship W3019833029A5059471349 @default.
• W3019833029 hasAuthorship W3019833029A5076671209 @default.
• W3019833029 hasAuthorship W3019833029A5078723577 @default.
• W3019833029 hasAuthorship W3019833029A5085591173 @default.
• W3019833029 hasBestOaLocation W30198330291 @default.
• W3019833029 hasConcept C105702510 @default.
• W3019833029 hasConcept C142724271 @default.
• W3019833029 hasConcept C171056886 @default.
• W3019833029 hasConcept C198826908 @default.
• W3019833029 hasConcept C203014093 @default.
• W3019833029 hasConcept C204787440 @default.
• W3019833029 hasConcept C2776164576 @default.
• W3019833029 hasConcept C2777077863 @default.
• W3019833029 hasConcept C2777562237 @default.
• W3019833029 hasConcept C2777564180 @default.
• W3019833029 hasConcept C2777652487 @default.
• W3019833029 hasConcept C2780550940 @default.
• W3019833029 hasConcept C28328180 @default.
• W3019833029 hasConcept C3020332539 @default.
• W3019833029 hasConcept C35496256 @default.
• W3019833029 hasConcept C71924100 @default.
• W3019833029 hasConcept C86803240 @default.
• W3019833029 hasConcept C95444343 @default.
• W3019833029 hasConceptScore W3019833029C105702510 @default.
• W3019833029 hasConceptScore W3019833029C142724271 @default.
• W3019833029 hasConceptScore W3019833029C171056886 @default.
• W3019833029 hasConceptScore W3019833029C198826908 @default.
• W3019833029 hasConceptScore W3019833029C203014093 @default.
• W3019833029 hasConceptScore W3019833029C204787440 @default.
• W3019833029 hasConceptScore W3019833029C2776164576 @default.
• W3019833029 hasConceptScore W3019833029C2777077863 @default.
• W3019833029 hasConceptScore W3019833029C2777562237 @default.
• W3019833029 hasConceptScore W3019833029C2777564180 @default.
• W3019833029 hasConceptScore W3019833029C2777652487 @default.
• W3019833029 hasConceptScore W3019833029C2780550940 @default.
• W3019833029 hasConceptScore W3019833029C28328180 @default.
• W3019833029 hasConceptScore W3019833029C3020332539 @default.
• W3019833029 hasConceptScore W3019833029C35496256 @default.
• W3019833029 hasConceptScore W3019833029C71924100 @default.
• W3019833029 hasConceptScore W3019833029C86803240 @default.
• W3019833029 hasConceptScore W3019833029C95444343 @default.
• W3019833029 hasLocation W30198330291 @default.
• W3019833029 hasOpenAccess W3019833029 @default.
• W3019833029 hasPrimaryLocation W30198330291 @default.
• W3019833029 hasRelatedWork W1544989653 @default.
• W3019833029 hasRelatedWork W2062253021 @default.
• W3019833029 hasRelatedWork W2141331032 @default.
• W3019833029 hasRelatedWork W2796861094 @default.
• W3019833029 hasRelatedWork W2797103110 @default.
• W3019833029 hasRelatedWork W3045025929 @default.
• W3019833029 hasRelatedWork W3138290288 @default.
• W3019833029 hasRelatedWork W3170575571 @default.
• W3019833029 hasRelatedWork W4365442757 @default.
• W3019833029 hasRelatedWork W65142845 @default.
• W3019833029 hasVolume "28" @default.
• W3019833029 isParatext "false" @default.
• W3019833029 isRetracted "false" @default.
• W3019833029 magId "3019833029" @default.
• W3019833029 workType "article" @default.