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- W3019848708 abstract "Glycans play an important role in various physiological and pathological processes. Metabolic labeling with bioorthogonal chemistry is a distinguished tool for detecting and tracking glycans in cells and in vivo. However, most of the currently available bioorthogonal turn-on probes based on organic fluorophores still suffer from some inevitable deficiencies, including shallow tissue penetration and spontaneous fluorescence. Herein, we designed and reported a bioorthogonal turn-on nanoprobe UCNP-T, which could realize the specific labeling and visualization of glycans on living cell membranes. UCNP-T was constructed based on a multi-spectral upconversion nanophosphor (UCNP) as the luminescence resonance energy transfer (LRET) donor and an organic molecule, tetrazine, as the acceptor. Using the as-prepared UCNP-T, we could specifically label the cell-surface glycans and monitor their level in living mice in real time through the ratio of upconversion luminescence (UCL) emissions of 540 nm to 650 nm (UCL540/UCL650), providing sensing with highly intrinsic reliability by self-calibration. Thus, the nanoprobe would provide a reliable tool for elucidating the role of glycosylation in cells and in vivo." @default.
- W3019848708 created "2020-05-01" @default.
- W3019848708 creator A5035701638 @default.
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- W3019848708 date "2020-01-01" @default.
- W3019848708 modified "2023-10-17" @default.
- W3019848708 title "<i>In vivo</i> selective imaging of metabolic glycosylation with a tetrazine-modified upconversion nanoprobe" @default.
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- W3019848708 doi "https://doi.org/10.1039/d0ra01832e" @default.
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