Matches in SemOpenAlex for { <https://semopenalex.org/work/W302000954> ?p ?o ?g. }
- W302000954 endingPage "3508" @default.
- W302000954 startingPage "3500" @default.
- W302000954 abstract "Abstract Residues within the first and fourth helices of human granulocyte- macrophage colony-stimulating factor (hGM-CSF) were analyzed for their role in biologic activity and interaction with the alpha- and beta- chains of the hGM-CSF receptor. Within the first helix substitution of the surface residues Glu14, Asn17, Gln20, Arg23, Arg24, and Asn27 or the buried residues Ala18, Leu25, and Leu28 did not significantly impair bioactivity or receptor binding. Substitutions at the buried residues Ala22 and Leu26 had intermediate bioactivity. However, substitutions of the surface residue Glu21 or the buried residue Ile19 reduced the relative bioactivity of the analogues to as little as 0.45% and 0.3%, respectively. Substitution of the charged surface residues of the fourth helix showed that substitution at Glu104, Lys107, and Lys111 had no significant effect on bioactivity, but substitution at Glu108 and Asp112 reduced the potency of the analogues to 34% and 7%, respectively. Receptor binding studies showed that, whereas Glu21 is the critical residue for binding to the hGM-CSF-receptor beta-chain, Asp112 is likely to be involved in binding to the GM-CSF-receptor alpha- chain. These results establish the relative contribution of residues in the first and fourth helices for GM-CSF bioactivity and receptor binding, and support a model where the fourth helix of GM-CSF interacts with the alpha-chain, and the first helix with the beta-chain of the GM- CSF receptor." @default.
- W302000954 created "2016-06-24" @default.
- W302000954 creator A5007565589 @default.
- W302000954 creator A5009240396 @default.
- W302000954 creator A5028262165 @default.
- W302000954 creator A5045060641 @default.
- W302000954 creator A5050442803 @default.
- W302000954 creator A5059823858 @default.
- W302000954 creator A5067370508 @default.
- W302000954 creator A5071049215 @default.
- W302000954 date "1994-06-15" @default.
- W302000954 modified "2023-09-27" @default.
- W302000954 title "Identification of residues in the first and fourth helices of human granulocyte-macrophage colony-stimulating factor involved in biologic activity and in binding to the alpha- and beta-chains of its receptor" @default.
- W302000954 cites W1502629197 @default.
- W302000954 cites W1513939725 @default.
- W302000954 cites W1547742399 @default.
- W302000954 cites W155286700 @default.
- W302000954 cites W1557721783 @default.
- W302000954 cites W1560065907 @default.
- W302000954 cites W1596747313 @default.
- W302000954 cites W16233902 @default.
- W302000954 cites W167658880 @default.
- W302000954 cites W188151362 @default.
- W302000954 cites W1963622614 @default.
- W302000954 cites W1976161079 @default.
- W302000954 cites W1977157092 @default.
- W302000954 cites W1980561488 @default.
- W302000954 cites W2025450446 @default.
- W302000954 cites W2025864476 @default.
- W302000954 cites W2036631709 @default.
- W302000954 cites W2040968837 @default.
- W302000954 cites W2043813981 @default.
- W302000954 cites W2047747212 @default.
- W302000954 cites W2052293758 @default.
- W302000954 cites W2058486772 @default.
- W302000954 cites W2067377498 @default.
- W302000954 cites W2076224430 @default.
- W302000954 cites W2080513999 @default.
- W302000954 cites W2096843631 @default.
- W302000954 cites W2098648394 @default.
- W302000954 cites W2107780898 @default.
- W302000954 cites W2135862623 @default.
- W302000954 cites W2139441497 @default.
- W302000954 cites W2158528246 @default.
- W302000954 cites W2211545673 @default.
- W302000954 cites W2309349704 @default.
- W302000954 cites W2335211451 @default.
- W302000954 cites W2342420586 @default.
- W302000954 cites W2410918592 @default.
- W302000954 cites W63256448 @default.
- W302000954 cites W984887640 @default.
- W302000954 doi "https://doi.org/10.1182/blood.v83.12.3500.3500" @default.
- W302000954 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8204877" @default.
- W302000954 hasPublicationYear "1994" @default.
- W302000954 type Work @default.
- W302000954 sameAs 302000954 @default.
- W302000954 citedByCount "63" @default.
- W302000954 countsByYear W3020009542012 @default.
- W302000954 countsByYear W3020009542013 @default.
- W302000954 countsByYear W3020009542014 @default.
- W302000954 countsByYear W3020009542015 @default.
- W302000954 countsByYear W3020009542016 @default.
- W302000954 countsByYear W3020009542018 @default.
- W302000954 countsByYear W3020009542020 @default.
- W302000954 countsByYear W3020009542023 @default.
- W302000954 crossrefType "journal-article" @default.
- W302000954 hasAuthorship W302000954A5007565589 @default.
- W302000954 hasAuthorship W302000954A5009240396 @default.
- W302000954 hasAuthorship W302000954A5028262165 @default.
- W302000954 hasAuthorship W302000954A5045060641 @default.
- W302000954 hasAuthorship W302000954A5050442803 @default.
- W302000954 hasAuthorship W302000954A5059823858 @default.
- W302000954 hasAuthorship W302000954A5067370508 @default.
- W302000954 hasAuthorship W302000954A5071049215 @default.
- W302000954 hasBestOaLocation W3020009541 @default.
- W302000954 hasConcept C107824862 @default.
- W302000954 hasConcept C152204938 @default.
- W302000954 hasConcept C170493617 @default.
- W302000954 hasConcept C184538305 @default.
- W302000954 hasConcept C185592680 @default.
- W302000954 hasConcept C18903297 @default.
- W302000954 hasConcept C197462201 @default.
- W302000954 hasConcept C202751555 @default.
- W302000954 hasConcept C2778530040 @default.
- W302000954 hasConcept C2779244956 @default.
- W302000954 hasConcept C2779965526 @default.
- W302000954 hasConcept C2781338088 @default.
- W302000954 hasConcept C47701112 @default.
- W302000954 hasConcept C50442881 @default.
- W302000954 hasConcept C55493867 @default.
- W302000954 hasConcept C64921476 @default.
- W302000954 hasConcept C71240020 @default.
- W302000954 hasConcept C86803240 @default.
- W302000954 hasConceptScore W302000954C107824862 @default.
- W302000954 hasConceptScore W302000954C152204938 @default.
- W302000954 hasConceptScore W302000954C170493617 @default.
- W302000954 hasConceptScore W302000954C184538305 @default.
- W302000954 hasConceptScore W302000954C185592680 @default.