FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3020183885> ?p ?o ?g. }

• W3020183885 abstract "ABSTRACT Levels of the transcription factor ATF6α, a key mediator of the unfolded protein response, that provides cellular protection during the progression endoplasmic reticulum (ER) stress, are markedly reduced in the pancreatic islet of patients with type 2 diabetes and in rodent models of the disease, including ob/ob and high fat-fed mice. Sorcin (gene name SRI ) is a calcium (Ca 2+ ) binding protein involved in maintaining ER Ca 2+ homeostasis. We have previously shown that overexpressing sorcin under the rat insulin promoter in transgenic mice was protective against high fat diet-induced pancreatic beta cell dysfunction, namely preserving intracellular Ca 2+ homeostasis and glucose-stimulated insulin secretion during lipotoxic stress. Additionally, sorcin overexpression was apparently activating ATF6 signalling in MIN6 cells despite lowering ER stress. Here, in order to investigate further the relationship between sorcin and ATF6, we describe changes in sorcin expression during ER and lipotoxic stress and changes in ATF6 signalling after sorcin overexpression or inactivation, both in excitable and non-excitable cells. Sorcin mRNA levels were significantly increased in response to the ER stress-inducing agents thapsigargin and tunicamycin, but not by palmitate. On the contrary, palmitate caused a significant decrease in sorcin expression as assessed by both qRT-PCR and Western blotting despite inducing ER stress. Moreover, palmitate prevented the increase in sorcin expression induced by thapsigargin. In addition, sorcin overexpression significantly increased ATF6 transcriptional activity, whereas sorcin inactivation decreased ATF6 signalling. Finally, sorcin overexpression increased levels of ATF6 immunoreactivity and FRET imaging experiments following ER stress induction by thapsigargin showed a direct sorcin-ATF6 interaction. Altogether, our data suggest that sorcin down-regulation during lipotoxicity may prevent full ATF6 activation and a normal UPR during the progression of obesity and insulin resistance, contributing to beta cell failure and type 2 diabetes." @default.
• W3020183885 created "2020-05-01" @default.
• W3020183885 creator A5000550995 @default.
• W3020183885 creator A5001913005 @default.
• W3020183885 creator A5010365898 @default.
• W3020183885 creator A5028326468 @default.
• W3020183885 creator A5031774412 @default.
• W3020183885 creator A5034011336 @default.
• W3020183885 creator A5035148818 @default.
• W3020183885 creator A5050833631 @default.
• W3020183885 creator A5055796292 @default.
• W3020183885 creator A5062235390 @default.
• W3020183885 creator A5067353830 @default.
• W3020183885 creator A5088435331 @default.
• W3020183885 date "2020-04-24" @default.
• W3020183885 modified "2023-09-27" @default.
• W3020183885 title "Sorcin stimulates Activation Transcription Factor 6α (ATF6) transcriptional activity" @default.
• W3020183885 cites W1546222819 @default.
• W3020183885 cites W1960734071 @default.
• W3020183885 cites W1968766433 @default.
• W3020183885 cites W1970420856 @default.
• W3020183885 cites W1973990965 @default.
• W3020183885 cites W1974992308 @default.
• W3020183885 cites W1977709885 @default.
• W3020183885 cites W1980931112 @default.
• W3020183885 cites W1983549722 @default.
• W3020183885 cites W1984325351 @default.
• W3020183885 cites W1986721511 @default.
• W3020183885 cites W1990057935 @default.
• W3020183885 cites W1996067419 @default.
• W3020183885 cites W1998662444 @default.
• W3020183885 cites W2003437932 @default.
• W3020183885 cites W2006877265 @default.
• W3020183885 cites W2007047797 @default.
• W3020183885 cites W2007078899 @default.
• W3020183885 cites W2012766451 @default.
• W3020183885 cites W2013127588 @default.
• W3020183885 cites W2013272984 @default.
• W3020183885 cites W2019550402 @default.
• W3020183885 cites W2022105849 @default.
• W3020183885 cites W2022548775 @default.
• W3020183885 cites W2033111397 @default.
• W3020183885 cites W2047628493 @default.
• W3020183885 cites W2049978305 @default.
• W3020183885 cites W2054979846 @default.
• W3020183885 cites W2055492712 @default.
• W3020183885 cites W2077757600 @default.
• W3020183885 cites W2078001940 @default.
• W3020183885 cites W2082515526 @default.
• W3020183885 cites W2089936541 @default.
• W3020183885 cites W2102440675 @default.
• W3020183885 cites W2105665965 @default.
• W3020183885 cites W2113708156 @default.
• W3020183885 cites W2116657339 @default.
• W3020183885 cites W2122647692 @default.
• W3020183885 cites W2124368774 @default.
• W3020183885 cites W2126859035 @default.
• W3020183885 cites W2127639686 @default.
• W3020183885 cites W2133644385 @default.
• W3020183885 cites W2133771702 @default.
• W3020183885 cites W2139259442 @default.
• W3020183885 cites W2142170772 @default.
• W3020183885 cites W2144065739 @default.
• W3020183885 cites W2147127785 @default.
• W3020183885 cites W2150549120 @default.
• W3020183885 cites W2151469610 @default.
• W3020183885 cites W2153974854 @default.
• W3020183885 cites W2162122379 @default.
• W3020183885 cites W2169612876 @default.
• W3020183885 cites W2226437153 @default.
• W3020183885 cites W2254382629 @default.
• W3020183885 cites W2255458792 @default.
• W3020183885 cites W2312537679 @default.
• W3020183885 cites W2400495271 @default.
• W3020183885 cites W2560195968 @default.
• W3020183885 cites W2767614677 @default.
• W3020183885 cites W2769834075 @default.
• W3020183885 cites W2902833346 @default.
• W3020183885 cites W2908615024 @default.
• W3020183885 cites W2938891386 @default.
• W3020183885 cites W39499692 @default.
• W3020183885 cites W4232836391 @default.
• W3020183885 doi "https://doi.org/10.1101/2020.04.23.053322" @default.
• W3020183885 hasPublicationYear "2020" @default.
• W3020183885 type Work @default.
• W3020183885 sameAs 3020183885 @default.
• W3020183885 citedByCount "1" @default.
• W3020183885 countsByYear W30201838852021 @default.
• W3020183885 crossrefType "posted-content" @default.
• W3020183885 hasAuthorship W3020183885A5000550995 @default.
• W3020183885 hasAuthorship W3020183885A5001913005 @default.
• W3020183885 hasAuthorship W3020183885A5010365898 @default.
• W3020183885 hasAuthorship W3020183885A5028326468 @default.
• W3020183885 hasAuthorship W3020183885A5031774412 @default.
• W3020183885 hasAuthorship W3020183885A5034011336 @default.
• W3020183885 hasAuthorship W3020183885A5035148818 @default.
• W3020183885 hasAuthorship W3020183885A5050833631 @default.
• W3020183885 hasAuthorship W3020183885A5055796292 @default.
• W3020183885 hasAuthorship W3020183885A5062235390 @default.
• W3020183885 hasAuthorship W3020183885A5067353830 @default.

• next