Matches in SemOpenAlex for { <https://semopenalex.org/work/W3020939618> ?p ?o ?g. }
Showing items 1 to 65 of
65
with 100 items per page.
- W3020939618 abstract "Publisher SummaryThis chapter examines the activity, specificity and structural chemistry of oligopeptidase MepB. MepB degrades the Pz-peptide substrate Pz-Pro-LeufGly-Pro-D-Arg. The enzyme was also reported to cleave native type I collagen. On the other hand, azocasein and azocoll were not hydrolyzed by MepB nor was FALeu-Gly-Pro-Ala a specific substrate for bacterial collagenolytic enzymes. The optimum pH for degrading the Pz-PLGPR substrate is 7.1. MepB is a single-chain protein of 82 kDa, pi 5.6. The amino acid sequence is closely similar to that of saccharolysin, and to mammalian thimet oligopeptidase, neurolysin, oligopeptidase A and peptidyl-dipeptidase. The most conserved region is found in the putative catalytic domain that contains two amino acid motifs, His-Glu-Leu-Gly-His, where the two His residues are assumed to be zinc ligands and the Glu residue may polarize the water molecule involved in nucleophilic attack on the scissile peptide bond, and Asp-Phe-Val-Glu-Ala-Pro, which contains a second Glu that may participate in zinc binding. An open reading frame of 2,061 bp interrupted by two introns was identified. It is found that despite the occurrence of two potential N-glycosylation sites in MepB, the purified protein appears not to be Af-glycosylated because it does not bind to concanavalin A." @default.
- W3020939618 created "2020-05-13" @default.
- W3020939618 creator A5022022597 @default.
- W3020939618 date "2004-01-01" @default.
- W3020939618 modified "2023-09-28" @default.
- W3020939618 title "Oligopeptidase MepB" @default.
- W3020939618 cites W1488354887 @default.
- W3020939618 cites W1598966730 @default.
- W3020939618 cites W1966444404 @default.
- W3020939618 cites W1968038037 @default.
- W3020939618 cites W1969340813 @default.
- W3020939618 cites W1977651472 @default.
- W3020939618 cites W2033493201 @default.
- W3020939618 cites W2042662964 @default.
- W3020939618 cites W2044797251 @default.
- W3020939618 cites W2048457862 @default.
- W3020939618 cites W2057757711 @default.
- W3020939618 cites W2094481702 @default.
- W3020939618 cites W2401281048 @default.
- W3020939618 doi "https://doi.org/10.1016/b978-0-12-079611-3.50101-4" @default.
- W3020939618 hasPublicationYear "2004" @default.
- W3020939618 type Work @default.
- W3020939618 sameAs 3020939618 @default.
- W3020939618 citedByCount "0" @default.
- W3020939618 crossrefType "book-chapter" @default.
- W3020939618 hasAuthorship W3020939618A5022022597 @default.
- W3020939618 hasConcept C104317684 @default.
- W3020939618 hasConcept C167625842 @default.
- W3020939618 hasConcept C181199279 @default.
- W3020939618 hasConcept C185592680 @default.
- W3020939618 hasConcept C2779281246 @default.
- W3020939618 hasConcept C2780043585 @default.
- W3020939618 hasConcept C41183919 @default.
- W3020939618 hasConcept C515207424 @default.
- W3020939618 hasConcept C55493867 @default.
- W3020939618 hasConcept C71240020 @default.
- W3020939618 hasConcept C80458899 @default.
- W3020939618 hasConceptScore W3020939618C104317684 @default.
- W3020939618 hasConceptScore W3020939618C167625842 @default.
- W3020939618 hasConceptScore W3020939618C181199279 @default.
- W3020939618 hasConceptScore W3020939618C185592680 @default.
- W3020939618 hasConceptScore W3020939618C2779281246 @default.
- W3020939618 hasConceptScore W3020939618C2780043585 @default.
- W3020939618 hasConceptScore W3020939618C41183919 @default.
- W3020939618 hasConceptScore W3020939618C515207424 @default.
- W3020939618 hasConceptScore W3020939618C55493867 @default.
- W3020939618 hasConceptScore W3020939618C71240020 @default.
- W3020939618 hasConceptScore W3020939618C80458899 @default.
- W3020939618 hasLocation W30209396181 @default.
- W3020939618 hasOpenAccess W3020939618 @default.
- W3020939618 hasPrimaryLocation W30209396181 @default.
- W3020939618 hasRelatedWork W1764151989 @default.
- W3020939618 hasRelatedWork W1984243890 @default.
- W3020939618 hasRelatedWork W2035847639 @default.
- W3020939618 hasRelatedWork W2058069508 @default.
- W3020939618 hasRelatedWork W2075636841 @default.
- W3020939618 hasRelatedWork W2090341942 @default.
- W3020939618 hasRelatedWork W2156390491 @default.
- W3020939618 hasRelatedWork W2383441513 @default.
- W3020939618 hasRelatedWork W2737698320 @default.
- W3020939618 hasRelatedWork W3201595080 @default.
- W3020939618 isParatext "false" @default.
- W3020939618 isRetracted "false" @default.
- W3020939618 magId "3020939618" @default.
- W3020939618 workType "book-chapter" @default.