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- W3021000349 abstract "Publisher SummaryThere are two ways to achieve the understanding of function of genes: one requires “knocking out” the function of the gene from the cell and the other involves introduction of the gene into the cell. There are a wide variety of methods available to introduce genetic material into cells. These include simple manipulations such as mixing high molecular weight DNA with calcium phosphate, DEAE-dextran, polylysine, or polyornithine. Other methods involve microinjection, electroporation, protoplast fusion, liposomes, reconstituted viral envelopes, and viral vectors. The various efficiencies of RNA transfection in different cell types could be due to differential transfectability, suboptimal lipofectin: RNA ratios, translational efficiency, or stability of messenger RNA (mRNA). The RNA transfection technique can be used to investigate various parameters influencing the translational efficiency of mRNAs by directly transfecting mRNA transcripts generated in vitro from DNA constructs containing specific sequences. RNA-lipofectin procedure has the ability to transfect a wide variety of cell types. This procedure can also be used to study the effect of various sequence elements on mRNA stability." @default.
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- W3021000349 date "1995-01-01" @default.
- W3021000349 modified "2023-09-27" @default.
- W3021000349 title "Cationic Liposome-Mediated RNA Transfection" @default.
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- W3021000349 doi "https://doi.org/10.1016/b978-0-12-765561-1.50041-2" @default.
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