FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3021124747> ?p ?o ?g. }

• W3021124747 endingPage "1179" @default.
• W3021124747 startingPage "1169" @default.
• W3021124747 abstract "Aims: Patients with metastatic colorectal carcinoma (mCRC) carrying activating mutations of the KRAS gene do not benefit from treatment with anti-EGF receptor monoclonal antibodies. Therefore, KRAS mutation testing of mCRC patients is mandatory in the clinical setting to aid in the choice of appropriate therapy. Materials & methods: We developed a cost-effective approach for the determination of KRAS mutations in codons 12 and 13 in clinical practice based on a sensitive PCR/sequencing technique and the commercially available real-time PCR-based Therascreen ® kit (DxS Ltd). Results & conclusion: The PCR/sequencing test was able to detect 10% mutant DNA in a background of wild-type DNA. By using this assay, we determined the mutational status of KRAS in 527 out of 540 (97.6%) formalin-fixed paraffin-embedded tissues from mCRC patients. PCR/sequencing was not conclusive in 13 cases, in which low-intensity peaks suggestive of potential mutations were identified. The DxS assay, which showed a sensitivity of 1%, identified mutations in 11 out of 13 inconclusive cases. Interestingly, five of these 11 cases showed high levels of DNA fragmentation. No significant difference was found in the ability of PCR/sequencing and DxS to identify KRAS mutations within 160 cases with more than 30% tumor cells. However, in 24 samples with less than 30% tumor cells, DxS showed an higher sensitivity. In conclusion, our findings suggest that PCR/sequencing can be used for mutational analysis of the majority of tumor samples that have more than 30% tumor cell content, whereas more sensitive and expensive tests should be reserved for inconclusive cases and for samples with a low amount of tumor cells." @default.
• W3021124747 created "2020-05-13" @default.
• W3021124747 creator A5003014678 @default.
• W3021124747 creator A5005851993 @default.
• W3021124747 creator A5023150524 @default.
• W3021124747 creator A5036367394 @default.
• W3021124747 creator A5038198711 @default.
• W3021124747 creator A5056034569 @default.
• W3021124747 creator A5065811679 @default.
• W3021124747 creator A5081955195 @default.
• W3021124747 creator A5089302271 @default.
• W3021124747 creator A5091765414 @default.
• W3021124747 date "2010-08-01" @default.
• W3021124747 modified "2023-10-11" @default.
• W3021124747 title "Detection of <i>KRAS</i> mutations in colorectal carcinoma patients with an integrated PCR/sequencing and real-time PCR approach" @default.
• W3021124747 cites W1653677725 @default.
• W3021124747 cites W1993739082 @default.
• W3021124747 cites W1997860952 @default.
• W3021124747 cites W1998152086 @default.
• W3021124747 cites W199842401 @default.
• W3021124747 cites W2001258296 @default.
• W3021124747 cites W2003278673 @default.
• W3021124747 cites W2005104372 @default.
• W3021124747 cites W2022304552 @default.
• W3021124747 cites W2038624343 @default.
• W3021124747 cites W2050597958 @default.
• W3021124747 cites W2083695927 @default.
• W3021124747 cites W2088293068 @default.
• W3021124747 cites W2095201384 @default.
• W3021124747 cites W2100421179 @default.
• W3021124747 cites W2106789440 @default.
• W3021124747 cites W2128059540 @default.
• W3021124747 cites W2143564662 @default.
• W3021124747 cites W2144425748 @default.
• W3021124747 cites W2155311724 @default.
• W3021124747 cites W2161516303 @default.
• W3021124747 cites W2163195607 @default.
• W3021124747 cites W2167539263 @default.
• W3021124747 cites W3148852979 @default.
• W3021124747 cites W4245112058 @default.
• W3021124747 doi "https://doi.org/10.2217/pgs.10.86" @default.
• W3021124747 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20712532" @default.
• W3021124747 hasPublicationYear "2010" @default.
• W3021124747 type Work @default.
• W3021124747 sameAs 3021124747 @default.
• W3021124747 citedByCount "40" @default.
• W3021124747 countsByYear W30211247472012 @default.
• W3021124747 countsByYear W30211247472013 @default.
• W3021124747 countsByYear W30211247472014 @default.
• W3021124747 countsByYear W30211247472015 @default.
• W3021124747 countsByYear W30211247472016 @default.
• W3021124747 countsByYear W30211247472017 @default.
• W3021124747 countsByYear W30211247472021 @default.
• W3021124747 countsByYear W30211247472023 @default.
• W3021124747 crossrefType "journal-article" @default.
• W3021124747 hasAuthorship W3021124747A5003014678 @default.
• W3021124747 hasAuthorship W3021124747A5005851993 @default.
• W3021124747 hasAuthorship W3021124747A5023150524 @default.
• W3021124747 hasAuthorship W3021124747A5036367394 @default.
• W3021124747 hasAuthorship W3021124747A5038198711 @default.
• W3021124747 hasAuthorship W3021124747A5056034569 @default.
• W3021124747 hasAuthorship W3021124747A5065811679 @default.
• W3021124747 hasAuthorship W3021124747A5081955195 @default.
• W3021124747 hasAuthorship W3021124747A5089302271 @default.
• W3021124747 hasAuthorship W3021124747A5091765414 @default.
• W3021124747 hasConcept C104317684 @default.
• W3021124747 hasConcept C121608353 @default.
• W3021124747 hasConcept C143065580 @default.
• W3021124747 hasConcept C153911025 @default.
• W3021124747 hasConcept C2781187634 @default.
• W3021124747 hasConcept C48023723 @default.
• W3021124747 hasConcept C49105822 @default.
• W3021124747 hasConcept C501734568 @default.
• W3021124747 hasConcept C502942594 @default.
• W3021124747 hasConcept C51679486 @default.
• W3021124747 hasConcept C526805850 @default.
• W3021124747 hasConcept C54355233 @default.
• W3021124747 hasConcept C86803240 @default.
• W3021124747 hasConceptScore W3021124747C104317684 @default.
• W3021124747 hasConceptScore W3021124747C121608353 @default.
• W3021124747 hasConceptScore W3021124747C143065580 @default.
• W3021124747 hasConceptScore W3021124747C153911025 @default.
• W3021124747 hasConceptScore W3021124747C2781187634 @default.
• W3021124747 hasConceptScore W3021124747C48023723 @default.
• W3021124747 hasConceptScore W3021124747C49105822 @default.
• W3021124747 hasConceptScore W3021124747C501734568 @default.
• W3021124747 hasConceptScore W3021124747C502942594 @default.
• W3021124747 hasConceptScore W3021124747C51679486 @default.
• W3021124747 hasConceptScore W3021124747C526805850 @default.
• W3021124747 hasConceptScore W3021124747C54355233 @default.
• W3021124747 hasConceptScore W3021124747C86803240 @default.
• W3021124747 hasIssue "8" @default.
• W3021124747 hasLocation W30211247471 @default.
• W3021124747 hasLocation W30211247472 @default.
• W3021124747 hasOpenAccess W3021124747 @default.
• W3021124747 hasPrimaryLocation W30211247471 @default.
• W3021124747 hasRelatedWork W2008053561 @default.
• W3021124747 hasRelatedWork W2590035663 @default.

• next