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• W3021206796 abstract "This chapter describes the chinchilla otitis media (OM) model, which has enhanced the understanding of every aspect of this common childhood disease, including pathogenesis, immunoprophylaxis, and therapy. This model includes various methods used to induce middle ear (ME) inflammation under conditions of acute bacterial and viral infection, chronic inflammation, and eustachian tube dysfunction. The chinchillas used in this model are housed in stainless-steel wire cages except during experiments involving inoculation of respiratory virus, when filterbonneted microisolator cages are used during the period of viral shedding. Prior to the start of an experiment, ears should be examined by tympanometry and otoscopy to verify the absence of middle-ear effusions (MEE) or tympanic membrane pathology. Chinchillas are effectively sedated with 10 mg ketamine hydrochloride injected into the anterior thigh. Complete anesthesia ensues in about 5 minutes and lasts for about 25 minutes. Chinchillas should be observed carefully while anesthetized as ketamine occasionally causes respiratory depression. Access to the chinchilla ME cavity is through the cephalad bulla underlying the dorsal aspect of the skull. The bone is very thin at the apex of the bulla, which allows to insert a needle into the ME cleft in a relatively atraumatic fashion. Chinchillas should be kept warm and observed closely until they have completely recovered from anesthesia. Following recovery, chinchillas return to normal behavior and are given food and water ad lib." @default.
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• W3021206796 date "1999-01-01" @default.
• W3021206796 modified "2023-09-26" @default.
• W3021206796 title "Otitis Media" @default.
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• W3021206796 doi "https://doi.org/10.1016/b978-012775390-4/50184-6" @default.
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