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- W3021245087 abstract "Abstract When stretched, cells cultured on 2D substrates share a universal softening and fluidization response that arises from poorly understood remodeling of well-conserved cytoskeletal elements. It is known, however, that the structure and distribution of the cytoskeleton is profoundly influenced by the dimensionality of a cell’s environment. Therefore, in this study we aimed to determine whether cells cultured in a 3D matrix share this softening behavior and to link it to cytoskeletal remodeling. To achieve this, we developed a high-throughput approach to measure the dynamic mechanical properties of cells and allow for sub-cellular imaging within physiologically relevant 3D microtissues. We found that fibroblast, smooth muscle and skeletal muscle microtissues strain softened but did not fluidize, and upon loading cessation, they regained their initial mechanical properties. Furthermore, microtissue prestress decreased with the strain amplitude to maintain a constant mean tension. This adaptation under an auxotonic condition resulted in lengthening. A filamentous actin cytoskeleton was required, and responses were mirrored by changes to actin remodeling rates and visual evidence of stretch-induced actin depolymerization. Our new approach for assessing cell mechanics has linked behaviors seen in 2D cultures to a 3D matrix, and connected remodeling of the cytoskeleton to homeostatic mechanical regulation of tissues." @default.
- W3021245087 created "2020-05-13" @default.
- W3021245087 creator A5017283956 @default.
- W3021245087 creator A5019124242 @default.
- W3021245087 creator A5027760538 @default.
- W3021245087 creator A5052205312 @default.
- W3021245087 date "2020-05-06" @default.
- W3021245087 modified "2023-10-17" @default.
- W3021245087 title "Structural and mechanical remodeling of the cytoskeleton maintains tensional homeostasis in 3D microtissues under acute dynamic stretch" @default.
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- W3021245087 doi "https://doi.org/10.1038/s41598-020-64725-7" @default.
- W3021245087 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7203149" @default.
- W3021245087 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32376876" @default.
- W3021245087 hasPublicationYear "2020" @default.
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