Matches in SemOpenAlex for { <https://semopenalex.org/work/W3021327677> ?p ?o ?g. }
- W3021327677 endingPage "443" @default.
- W3021327677 startingPage "433" @default.
- W3021327677 abstract "Respiratory mucosa defects result in airway obstruction and infection, requiring subsequent functional recovery of the respiratory epithelium. Because site-specific extracellular matrix (ECM) facilitates restoration of organ function by promoting cellular migration and engraftment, previous studies considered decellularized trachea an ideal ECM; however, incomplete cell removal from cartilage and mucosal-architecture destruction are frequently reported. Here, we developed a decellularization protocol and applied it to the respiratory mucosa of separated porcine tracheas. The trachea was divided into groups according to decellularization protocol: native mucosa, freezing–thawing (FT), FT followed by the use of Perasafe-based chemical agents before mucosal separation (wFTP), after mucosal separation (mFTP), and followed by DNase decellularization (mFTD). Decellularization efficacy was evaluated by DNA quantification and hematoxylin and eosin staining, and ECM content of the scaffold was evaluated by histologic analysis and glycosaminoglycan and collagen assays. Biocompatibility was assessed by cell-viability assay and in vivo transplantation. The mFTP mucosa showed low antigenicity and maintained the ECM to form a proper microstructure. Additionally, tonsil-derived stem cells remained viable when cultured with or seeded onto mFTP mucosa, and the in vivo host response showed a constructive pattern following implantation of the mFTP scaffolds. These results demonstrated that xenogenic acellular respiratory mucosa matrix displayed suitable biocompatibility as a scaffold material for respiratory mucosa engineering." @default.
- W3021327677 created "2020-05-13" @default.
- W3021327677 creator A5032728166 @default.
- W3021327677 creator A5039645150 @default.
- W3021327677 creator A5058540984 @default.
- W3021327677 creator A5070906331 @default.
- W3021327677 creator A5085509535 @default.
- W3021327677 date "2020-05-10" @default.
- W3021327677 modified "2023-09-30" @default.
- W3021327677 title "Development of Acellular Respiratory Mucosal Matrix Using Porcine Tracheal Mucosa" @default.
- W3021327677 cites W1845847174 @default.
- W3021327677 cites W1848787449 @default.
- W3021327677 cites W1969371115 @default.
- W3021327677 cites W1971189453 @default.
- W3021327677 cites W1987005299 @default.
- W3021327677 cites W1999558248 @default.
- W3021327677 cites W2032951807 @default.
- W3021327677 cites W2043557032 @default.
- W3021327677 cites W2051858469 @default.
- W3021327677 cites W2059486645 @default.
- W3021327677 cites W2065387405 @default.
- W3021327677 cites W2068305554 @default.
- W3021327677 cites W2068782007 @default.
- W3021327677 cites W2079867843 @default.
- W3021327677 cites W2079973249 @default.
- W3021327677 cites W2080638167 @default.
- W3021327677 cites W2116397695 @default.
- W3021327677 cites W2125602705 @default.
- W3021327677 cites W2141305039 @default.
- W3021327677 cites W2158722130 @default.
- W3021327677 cites W2189806069 @default.
- W3021327677 cites W2271800222 @default.
- W3021327677 cites W2324492433 @default.
- W3021327677 cites W2405152377 @default.
- W3021327677 cites W2467524730 @default.
- W3021327677 cites W2542479171 @default.
- W3021327677 cites W2587160918 @default.
- W3021327677 cites W2612232971 @default.
- W3021327677 cites W2620361336 @default.
- W3021327677 cites W2737850503 @default.
- W3021327677 cites W4237138030 @default.
- W3021327677 cites W2157820328 @default.
- W3021327677 doi "https://doi.org/10.1007/s13770-020-00260-w" @default.
- W3021327677 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7392986" @default.
- W3021327677 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32390116" @default.
- W3021327677 hasPublicationYear "2020" @default.
- W3021327677 type Work @default.
- W3021327677 sameAs 3021327677 @default.
- W3021327677 citedByCount "3" @default.
- W3021327677 countsByYear W30213276772021 @default.
- W3021327677 countsByYear W30213276772023 @default.
- W3021327677 crossrefType "journal-article" @default.
- W3021327677 hasAuthorship W3021327677A5032728166 @default.
- W3021327677 hasAuthorship W3021327677A5039645150 @default.
- W3021327677 hasAuthorship W3021327677A5058540984 @default.
- W3021327677 hasAuthorship W3021327677A5070906331 @default.
- W3021327677 hasAuthorship W3021327677A5085509535 @default.
- W3021327677 hasBestOaLocation W30213276772 @default.
- W3021327677 hasConcept C105702510 @default.
- W3021327677 hasConcept C136229726 @default.
- W3021327677 hasConcept C141071460 @default.
- W3021327677 hasConcept C142724271 @default.
- W3021327677 hasConcept C185592680 @default.
- W3021327677 hasConcept C189165786 @default.
- W3021327677 hasConcept C190672674 @default.
- W3021327677 hasConcept C203014093 @default.
- W3021327677 hasConcept C2780269544 @default.
- W3021327677 hasConcept C2780550940 @default.
- W3021327677 hasConcept C2910059436 @default.
- W3021327677 hasConcept C2911091166 @default.
- W3021327677 hasConcept C49892992 @default.
- W3021327677 hasConcept C529295009 @default.
- W3021327677 hasConcept C71924100 @default.
- W3021327677 hasConcept C86803240 @default.
- W3021327677 hasConcept C95444343 @default.
- W3021327677 hasConceptScore W3021327677C105702510 @default.
- W3021327677 hasConceptScore W3021327677C136229726 @default.
- W3021327677 hasConceptScore W3021327677C141071460 @default.
- W3021327677 hasConceptScore W3021327677C142724271 @default.
- W3021327677 hasConceptScore W3021327677C185592680 @default.
- W3021327677 hasConceptScore W3021327677C189165786 @default.
- W3021327677 hasConceptScore W3021327677C190672674 @default.
- W3021327677 hasConceptScore W3021327677C203014093 @default.
- W3021327677 hasConceptScore W3021327677C2780269544 @default.
- W3021327677 hasConceptScore W3021327677C2780550940 @default.
- W3021327677 hasConceptScore W3021327677C2910059436 @default.
- W3021327677 hasConceptScore W3021327677C2911091166 @default.
- W3021327677 hasConceptScore W3021327677C49892992 @default.
- W3021327677 hasConceptScore W3021327677C529295009 @default.
- W3021327677 hasConceptScore W3021327677C71924100 @default.
- W3021327677 hasConceptScore W3021327677C86803240 @default.
- W3021327677 hasConceptScore W3021327677C95444343 @default.
- W3021327677 hasFunder F4320321365 @default.
- W3021327677 hasFunder F4320322349 @default.
- W3021327677 hasIssue "4" @default.
- W3021327677 hasLocation W30213276771 @default.
- W3021327677 hasLocation W30213276772 @default.
- W3021327677 hasOpenAccess W3021327677 @default.